PMID- 32037657
OWN - NLM
STAT- MEDLINE
DCOM- 20210121
LR  - 20210121
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 34
IP  - 3
DP  - 2020 Mar
TI  - miR-382-5p promotes porcine reproductive and respiratory syndrome virus (PRRSV) 
      replication by negatively regulating the induction of type I interferon.
PG  - 4497-4511
LID - 10.1096/fj.201902031RRR [doi]
AB  - Previous studies have indicated that inhibition of type I interferon production 
      may be an important reason for porcine reproductive and respiratory syndrome 
      virus (PRRSV) to achieve immune escape, revealing the mechanism of inhibiting the 
      production of type I interferon will help design novel strategies for controlling 
      PRRS. Here, we found that PRRSV infection upregulated the expression of 
      miR-382-5p, which in turn inhibited polyI:C-induced the production of type I 
      interferon by targeting heat shock protein 60 (HSP60), thus facilitating PRRSV 
      replication in MARC-145 cells. Furthermore, we found that HSP60 could interact 
      with mitochondrial antiviral signaling protein (MAVS), an important signal 
      transduction protein for inducing production of type I interferon, and promote 
      polyI:C-mediated the production of type I interferon in a MAVS-dependent manner. 
      Finally, we also found that HSP60 could inhibit PRRSV replication in a 
      MAVS-dependent manner, which indicated that HSP60 was a novel antiviral protein 
      against PRRSV replication. In conclusion, the study demonstrated that miR-382-5p 
      was upregulated during PRRSV infection and may promote PRRSV replication by 
      negatively regulating the production of type I interferon, which also indicated 
      that miR-382-5p and HSP60 might be the potential therapeutic targets for 
      anti-PRRSV.
CI  - (c) 2020 Federation of American Societies for Experimental Biology.
FAU - Chang, Xiaobo
AU  - Chang X
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou, China.
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, China.
FAU - Shi, Xibao
AU  - Shi X
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou, China.
AD  - College of Life Sciences, Henan Normal University, Xinxiang, China.
FAU - Zhang, Xiaozhuan
AU  - Zhang X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, China.
FAU - Chen, Jing
AU  - Chen J
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou, China.
FAU - Fan, Xiaomin
AU  - Fan X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, China.
FAU - Yang, Yuanhao
AU  - Yang Y
AD  - College of Life Sciences, Henan Normal University, Xinxiang, China.
FAU - Wang, Li
AU  - Wang L
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, China.
FAU - Deng, Ruiguang
AU  - Deng R
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou, China.
FAU - Zhou, Enmin
AU  - Zhou E
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, China.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou, China.
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, China.
AD  - College of Animal Science and Veterinary Medicine, Henan Agricultural University, 
      Zhengzhou, China.
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200210
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Chaperonin 60)
RN  - 0 (Interferon Type I)
RN  - 0 (MicroRNAs)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chaperonin 60/genetics/*metabolism
MH  - Cricetinae
MH  - HEK293 Cells
MH  - Host-Pathogen Interactions/genetics/physiology
MH  - Humans
MH  - Interferon Type I/genetics/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Plasmids/genetics
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - RNA Interference
MH  - Viral Nonstructural Proteins/genetics/metabolism
MH  - Virus Replication/genetics/physiology
OTO - NOTNLM
OT  - HSP60
OT  - MAVS
OT  - antiviral protein
EDAT- 2020/02/11 06:00
MHDA- 2021/01/22 06:00
CRDT- 2020/02/11 06:00
PHST- 2019/08/12 00:00 [received]
PHST- 2020/01/14 00:00 [revised]
PHST- 2020/01/14 00:00 [accepted]
PHST- 2020/02/11 06:00 [pubmed]
PHST- 2021/01/22 06:00 [medline]
PHST- 2020/02/11 06:00 [entrez]
AID - 10.1096/fj.201902031RRR [doi]
PST - ppublish
SO  - FASEB J. 2020 Mar;34(3):4497-4511. doi: 10.1096/fj.201902031RRR. Epub 2020 Feb 
      10.