PMID- 32041908
OWN - NLM
STAT- MEDLINE
DCOM- 20210201
LR  - 20210201
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 182
IP  - 4
DP  - 2020 Apr
TI  - Pyrenoid Starch Sheath Is Required for LCIB Localization and the 
      CO(2)-Concentrating Mechanism in Green Algae.
PG  - 1883-1893
LID - 10.1104/pp.19.01587 [doi]
AB  - Aquatic photosynthetic organisms induce a CO(2)-concentrating mechanism (CCM) to 
      overcome the difficulty of acquiring inorganic carbon under CO(2)-limiting 
      conditions. As part of the CCM, the CO(2)-fixing enzyme Rubisco is enriched in 
      the pyrenoid located in the chloroplast, and, in many green algae, several thick 
      starch plates surround the pyrenoid to form a starch sheath. In Chlamydomonas 
      reinhardtii, low-CO(2)-inducible protein B (LCIB), which is an essential factor 
      for the CCM, displays altered cellular localization in response to a decrease in 
      environmental CO(2) concentration, moving from dispersed throughout the 
      chloroplast stroma to around the pyrenoid. However, the mechanism behind LCIB 
      migration remains poorly understood. Here, we report the characteristics of an 
      Isoamylase1-less mutant (4-D1), which shows aberrant LCIB localization and starch 
      sheath formation. Under very-low-CO(2) conditions, 4-D1 showed retarded growth, 
      lower photosynthetic affinities against inorganic carbon, and a decreased 
      accumulation level of the HCO(3) (-) transporter HLA3. The aberrant localization 
      of LCIB was also observed in another starch-sheathless mutant sta11-1, but not in 
      sta2-1, which possesses a thinned starch sheath. These results suggest that the 
      starch sheath around the pyrenoid is required for the correct localization of 
      LCIB and for the operation of CCM.
CI  - (c) 2020 American Society of Plant Biologists. All Rights Reserved.
FAU - Toyokawa, Chihana
AU  - Toyokawa C
AUID- ORCID: 0000-0001-5664-7565
AD  - Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
FAU - Yamano, Takashi
AU  - Yamano T
AUID- ORCID: 0000-0001-6578-8095
AD  - Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
FAU - Fukuzawa, Hideya
AU  - Fukuzawa H
AUID- ORCID: 0000-0003-0963-5662
AD  - Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan 
      fukuzawa@lif.kyoto-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200210
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Bicarbonates)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 9005-25-8 (Starch)
SB  - IM
CIN - Plant Physiol. 2020 Apr;182(4):1796-1797. PMID: 32253326
MH  - Bicarbonates/*metabolism
MH  - Carbon Dioxide/*metabolism
MH  - Chlamydomonas reinhardtii/metabolism
MH  - Chlorophyta/*metabolism
MH  - Starch/*metabolism
PMC - PMC7140920
EDAT- 2020/02/12 06:00
MHDA- 2021/02/02 06:00
PMCR- 2020/02/10
CRDT- 2020/02/12 06:00
PHST- 2020/01/03 00:00 [received]
PHST- 2020/01/24 00:00 [accepted]
PHST- 2020/02/12 06:00 [pubmed]
PHST- 2021/02/02 06:00 [medline]
PHST- 2020/02/12 06:00 [entrez]
PHST- 2020/02/10 00:00 [pmc-release]
AID - pp.19.01587 [pii]
AID - 201901587R1 [pii]
AID - 10.1104/pp.19.01587 [doi]
PST - ppublish
SO  - Plant Physiol. 2020 Apr;182(4):1883-1893. doi: 10.1104/pp.19.01587. Epub 2020 Feb 
      10.