PMID- 32045312 OWN - NLM STAT- MEDLINE DCOM- 20211011 LR - 20211011 IS - 1532-4303 (Electronic) IS - 0277-0903 (Linking) VI - 58 IP - 6 DP - 2021 Jun TI - Expression of herpesvirus entry mediator gene as a potential biomarker for disease severity in patients with persistent asthma. PG - 717-724 LID - 10.1080/02770903.2020.1729382 [doi] AB - Objectives: Herpes virus entry mediator (HVEM) is a costimulatory molecule, and has been proved to play an important role in airway inflammatory and remodeling processes of asthma. We aimed to investigate the expression of HVEM gene in patients with asthma as a means of assessing disease severity.Methods: This study was carried out on 59 subjects, 16 patients with mild persistent asthma, 11 patients with moderate persistent asthma, 13 patients with severe persistent asthma, and 19 age and gender matched healthy controls. The HVEM mRNA expressions of all subjects were determined by real time PCR. Correlations between HVEM mRNA expression and fractional exhaled nitric oxide (FeNO), pulmonary function test values, total blood white cell count and differential, total immunoglobulin E (IgE) level, and Asthma Control Test (ACT) score were analyzed, respectively. The discrimination abilities of HVEM mRNA between different groups were tested using receiver operating characteristics (ROC) curve analyses.Results: This study showed the expressions of HVEM mRNA were significantly higher in the patients with severe and moderate persistent asthma than in patients with mild persistent asthma and healthy subjects (2.97 +/- 1.23 vs. 1.17 +/- 0.42 vs. 0.62 +/- 0.38 vs. 0.46 +/- 0.18/NAPDH, p < 0.001), but there was no significant difference between patients with mild persistent asthma and health controls (0.62 +/- 0.38 vs. 0.46 +/- 0.18/NAPDH, p = 0.557). HVEM mRNA expression at cut off point [1.01/NAPDH, area under the ROC curve (AUC) = 0.99] is sufficient to discriminate severe patients from mild-to-moderate patients, and at cut off point (0.93/NAPDH, AUC = 0.91) for discrimination of moderate-to-severe patients from mild ones, while at cut off point (0.76/NAPDH, AUC = 0.75) for discrimination of asthmatic patients from controls. Furthermore, HVEM mRNA expression was positively correlated with FeNO level (r = 0.524, p = 0.015), and total lymphocyte count (r = 0.426, p = 0.017) in patients with persistent asthma.Conclusions: HVEM gene expressions can be used as a potential biomarker for evaluating the severity of patients with persistent asthma. FAU - Xiong, Yi AU - Xiong Y AD - Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, China. FAU - Li, Binbin AU - Li B AD - Emergency Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Zhang, Yidan AU - Zhang Y AD - Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, China. FAU - Shi, Fei AU - Shi F AUID- ORCID: 0000-0002-9082-9140 AD - Emergency Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Qiu, Chen AU - Qiu C AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Wang, Lingwei AU - Wang L AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Wang, Jin AU - Wang J AD - Emergency Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Le, Ying AU - Le Y AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Du, Yujie AU - Du Y AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Yao, Can AU - Yao C AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Li, Sinian AU - Li S AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Liu, Wenwen AU - Liu W AD - Emergency Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Chen, Dandan AU - Chen D AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. FAU - Feng, Mengjie AU - Feng M AD - Respiratory Department, Shenzhen Institute of Respiratory Diseases, Jinan University, The Second Clinical College, Shenzhen, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200221 PL - England TA - J Asthma JT - The Journal of asthma : official journal of the Association for the Care of Asthma JID - 8106454 RN - 0 (Biomarkers) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Tumor Necrosis Factor, Member 14) RN - 0 (TNFRSF14 protein, human) RN - 31C4KY9ESH (Nitric Oxide) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adult MH - Asthma/*genetics/*physiopathology MH - Biomarkers MH - Breath Tests MH - Female MH - Humans MH - Immunoglobulin E/blood MH - Leukocyte Count MH - Male MH - Middle Aged MH - Nitric Oxide/analysis MH - RNA, Messenger MH - ROC Curve MH - Real-Time Polymerase Chain Reaction MH - Receptors, Tumor Necrosis Factor, Member 14/*biosynthesis MH - Respiratory Function Tests MH - Severity of Illness Index OTO - NOTNLM OT - Asthma OT - FeNO OT - HVEM OT - lymphocyte OT - severity EDAT- 2020/02/12 06:00 MHDA- 2021/10/12 06:00 CRDT- 2020/02/12 06:00 PHST- 2020/02/12 06:00 [pubmed] PHST- 2021/10/12 06:00 [medline] PHST- 2020/02/12 06:00 [entrez] AID - 10.1080/02770903.2020.1729382 [doi] PST - ppublish SO - J Asthma. 2021 Jun;58(6):717-724. doi: 10.1080/02770903.2020.1729382. Epub 2020 Feb 21.