PMID- 32046080 OWN - NLM STAT- MEDLINE DCOM- 20201118 LR - 20201118 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 25 IP - 3 DP - 2020 Feb 7 TI - Quality Enhancement Mechanism of Alkali-Free Chinese Northern Steamed Bread by Sourdough Acidification. LID - 10.3390/molecules25030726 [doi] LID - 726 AB - Alkali was used to adjust the pH and neutralize the excess acids of dough in the processing of Chinese northern steamed bread (CNSB). However, extra alkali addition generally resulted in alkalic flavor and poor appearance. The aim of this work was to investigate the role of proofed dough pH on the texture of CNSB. Correlation analysis demonstrated that the pH value of proofed dough has a significant effect on the textural properties of CNSB. The mechanism studies found that gradual acidification of dough by lactic acid bacteria is a critical factor affecting the process. Conversely, chemical acidification weakened the texture property of products and reduced the dough rheology. Scanning electron microscope (SEM) analysis showed that fermentation with starter for 12 h produced a continuous and extensional protein network in the proofed dough. Furthermore, the decreasing pH of proofed dough increased the extractability of protein in a sodium dodecyl sulfate (SDS)-containing medium and the content of free sulfhydryl (SH). The structure and content of gluten, especially influenced by gradual acidification level, change the quality of the final product. It is a novel approach to obtain an alkali-free CNSB with excellent quality by moderate gluten adjustment. FAU - Yan, Bowen AU - Yan B AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. FAU - Yang, Huayu AU - Yang H AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. FAU - Wu, Yejun AU - Wu Y AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. FAU - Lian, Huizhang AU - Lian H AD - Wuxi Huashun Minsheng Food Co. Ltd., Wuxi 214218, China. FAU - Zhang, Hao AU - Zhang H AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. FAU - Chen, Wei AU - Chen W AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. FAU - Fan, Daming AU - Fan D AUID- ORCID: 0000-0002-4907-9034 AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. FAU - Zhao, Jianxin AU - Zhao J AD - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. AD - National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China. LA - eng GR - 31871841/National Natural Science Foundation of China/ GR - KYCX19_1865/Postgraduate Research & Practice Innovation Program of Jiangsu Province/ PT - Journal Article DEP - 20200207 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Acids) RN - 0 (Alkalies) RN - 0 (Proteins) RN - 0 (Steam) RN - 8002-80-0 (Glutens) SB - IM MH - Acids/*chemistry MH - Alkalies/*chemistry MH - Bread/*microbiology MH - Fermentation/physiology MH - Flour/microbiology MH - Food Technology/methods MH - Glutens/chemistry MH - Hydrogen-Ion Concentration MH - Lactobacillales/metabolism MH - Microscopy, Electron, Scanning/methods MH - Proteins/chemistry MH - Rheology MH - Steam MH - Triticum/chemistry PMC - PMC7037005 OTO - NOTNLM OT - Chinese steamed bread OT - acidification OT - gluten OT - sourdough COIS- The authors declare no conflict of interest. EDAT- 2020/02/13 06:00 MHDA- 2020/11/20 06:00 PMCR- 2020/02/07 CRDT- 2020/02/13 06:00 PHST- 2019/12/23 00:00 [received] PHST- 2020/02/05 00:00 [revised] PHST- 2020/02/06 00:00 [accepted] PHST- 2020/02/13 06:00 [entrez] PHST- 2020/02/13 06:00 [pubmed] PHST- 2020/11/20 06:00 [medline] PHST- 2020/02/07 00:00 [pmc-release] AID - molecules25030726 [pii] AID - molecules-25-00726 [pii] AID - 10.3390/molecules25030726 [doi] PST - epublish SO - Molecules. 2020 Feb 7;25(3):726. doi: 10.3390/molecules25030726.