PMID- 32046264
OWN - NLM
STAT- MEDLINE
DCOM- 20200914
LR  - 20200914
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 3
DP  - 2020 Feb 8
TI  - IL-33 Inhibits TNF-alpha-Induced Osteoclastogenesis and Bone Resorption.
LID - 10.3390/ijms21031130 [doi]
LID - 1130
AB  - Interleukin (IL)-33 is a member of the IL-1 family, which acts as an alarmin. 
      Several studies suggested that IL-33 inhibited osteoclastogenesis and bone 
      resorption. Tumor necrosis factor-alpha (TNF-alpha) is considered a direct inducer of 
      osteoclastogenesis. However, there has been no report regarding the effect of 
      IL-33 on TNF-alpha-induced osteoclastogenesis and bone resorption. The objective of 
      this study is to investigate the role of IL-33 on TNF-alpha-induced 
      osteoclastogenesis and bone resorption. In an in vitro analysis of 
      osteoclastogenesis, osteoclast precursors, which were derived from bone marrow 
      cells, were treated with or without IL-33 in the presence of TNF-alpha. 
      Tartrate-resistant acid phosphatase (TRAP) staining solution was used to assess 
      osteoclast formation. In an in vivo analysis of mouse calvariae, TNF-alpha with or 
      without IL-33 was subcutaneously administrated into the supracalvarial region of 
      mice daily for 5 days. Histological sections were stained for TRAP, and 
      osteoclast numbers were determined. Using micro-CT reconstruction images, the 
      ratio of bone destruction area on the calvariae was evaluated. The number of 
      TRAP-positive cells induced by TNF-alpha was significantly decreased with IL-33 in 
      vitro and in vivo. Bone resorption was also reduced. IL-33 inhibited IkappaB 
      phosphorylation and NF-kappaB nuclear translocation. These results suggest that IL-33 
      inhibited TNF-alpha-induced osteoclastogenesis and bone resorption.
FAU - Ohori, Fumitoshi
AU  - Ohori F
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Kitaura, Hideki
AU  - Kitaura H
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Ogawa, Saika
AU  - Ogawa S
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Shen, Wei-Ren
AU  - Shen WR
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Qi, Jiawei
AU  - Qi J
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Noguchi, Takahiro
AU  - Noguchi T
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Marahleh, Aseel
AU  - Marahleh A
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Nara, Yasuhiko
AU  - Nara Y
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Pramusita, Adya
AU  - Pramusita A
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
FAU - Mizoguchi, Itaru
AU  - Mizoguchi I
AD  - Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate 
      School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
LA  - eng
GR  - 16K11776/Japan Society for the Promotion of Science/
GR  - 19K10397/Japan Society for the Promotion of Science/
GR  - 18K09862/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20200208
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Interleukin-33)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Bone Resorption/*chemically induced/*drug therapy
MH  - Fluorescent Antibody Technique
MH  - Immunoblotting
MH  - Interleukin-33/*pharmacology/*therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - Osteoclasts/*cytology/*drug effects/metabolism
MH  - Osteogenesis/*drug effects
MH  - Phosphorylation/drug effects
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
PMC - PMC7038169
OTO - NOTNLM
OT  - IL-33
OT  - TNF-alpha
OT  - osteoclast
OT  - osteoclastogenesis
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/13 06:00
MHDA- 2020/09/15 06:00
PMCR- 2020/02/01
CRDT- 2020/02/13 06:00
PHST- 2019/12/25 00:00 [received]
PHST- 2020/02/03 00:00 [revised]
PHST- 2020/02/05 00:00 [accepted]
PHST- 2020/02/13 06:00 [entrez]
PHST- 2020/02/13 06:00 [pubmed]
PHST- 2020/09/15 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - ijms21031130 [pii]
AID - ijms-21-01130 [pii]
AID - 10.3390/ijms21031130 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Feb 8;21(3):1130. doi: 10.3390/ijms21031130.