PMID- 32046402
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20210110
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Print)
IS  - 1933-7191 (Linking)
VI  - 27
IP  - 3
DP  - 2020 Mar
TI  - HHV-6A Infection of Endometrial Epithelial Cells Affects miRNA Expression and 
      Trophoblast Cell Attachment.
PG  - 779-786
LID - 10.1007/s43032-019-00102-8 [doi]
AB  - We recently reported that human herpesvirus 6 (HHV-6) infection is frequently 
      present in endometrial tissue of women with unexplained infertility, and that 
      virus infection induces a profound remodulation of miRNA expression in human 
      cells of different origin. Since specific miRNA patterns have been associated 
      with specific pregnancy outcomes, we aimed to analyze the impact of HHV-6A 
      infection on miRNAs expression and trophoblast receptivity in human endometrial 
      cells. To this purpose, a human endometrial cell line (HEC-1A) was infected with 
      HHV-6A and analyzed for alterations in the expression of miRNAs and for 
      permissiveness to the attachment of a human choriocarcinoma trophoblast cell line 
      (JEG-3). The results showed that HHV-6A infection of endometrial cells 
      up-modulates miR22 (26-fold), miR15 (19.5-fold), and miR196-5p (12.1 fold), that 
      are correlated with implant failure, and down-modulates miR18 (11.4 fold), 
      miR101-3p (4.6 fold), miR181-5p (4.9 fold), miR92 (3.3 fold), and miR1207-5p (3.9 
      fold), characterized by a low expression in preeclampsia. Moreover, 
      HHV-6A-infected endometrial cells infected resulted less permissive to the 
      attachment of trophoblast cells. In conclusion, collected data suggest that 
      HHV-6A infection could modify miRNA expression pattern and control of trophoblast 
      cell adhesion of endometrial cells, undermining a correct trophoblast cell 
      attachment on endometrial cells.
FAU - Bortolotti, Daria
AU  - Bortolotti D
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - Soffritti, Irene
AU  - Soffritti I
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - D'Accolti, Maria
AU  - D'Accolti M
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - Gentili, Valentina
AU  - Gentili V
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - Di Luca, Dario
AU  - Di Luca D
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - Rizzo, Roberta
AU  - Rizzo R
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy.
FAU - Caselli, Elisabetta
AU  - Caselli E
AD  - Section of Microbiology and Medical Genetics, Department of Medical Sciences, 
      University of Ferrara, via Luigi Borsari 46, 44121, Ferrara, Italy. csb@unife.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200101
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (MicroRNAs)
SB  - IM
MH  - *Cell Adhesion
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Choriocarcinoma/metabolism/virology
MH  - Endometrium/metabolism/*virology
MH  - Epithelial Cells/metabolism/*virology
MH  - Female
MH  - Herpesvirus 6, Human/*metabolism
MH  - Humans
MH  - MicroRNAs/*metabolism
MH  - Roseolovirus Infections/*metabolism
MH  - Trophoblasts/metabolism/*virology
MH  - Uterine Neoplasms/metabolism/virology
PMC - PMC7077927
OTO - NOTNLM
OT  - Endometrium
OT  - HHV-6A
OT  - Infection
OT  - Pregnancy
OT  - miRNA
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/02/13 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/01/01
CRDT- 2020/02/13 06:00
PHST- 2019/05/27 00:00 [received]
PHST- 2019/08/13 00:00 [accepted]
PHST- 2020/02/13 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/02/13 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.1007/s43032-019-00102-8 [pii]
AID - 102 [pii]
AID - 10.1007/s43032-019-00102-8 [doi]
PST - ppublish
SO  - Reprod Sci. 2020 Mar;27(3):779-786. doi: 10.1007/s43032-019-00102-8. Epub 2020 
      Jan 1.