PMID- 32046454
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20201215
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Print)
IS  - 1933-7191 (Linking)
VI  - 27
IP  - 5
DP  - 2020 May
TI  - Upregulation of HLA-Class I and II in Placentas Diagnosed with Villitis of 
      Unknown Etiology.
PG  - 1129-1138
LID - 10.1007/s43032-019-00101-9 [doi]
AB  - The placenta utilizes many mechanisms to protect the haploidentical fetus from 
      recognition by the maternal immune system. However, in cases of villitis of 
      unknown etiology (VUE), maternal lymphocytes gain access into the placenta, 
      causing significant health risks for the fetus. Evidence suggests that VUE is a 
      rejection response between the mother and the haploidentical fetus. Therefore, we 
      profiled human leukocyte antigen (HLA), an important predictor of transplant 
      rejection, in VUE using placental tissue from ten patients with VUE and ten 
      gestational age matched controls. Placentas were stained using novel multiplexed 
      immunofluorescence (MxIF) to investigate morphology and HLA classes I and II. 
      Gene expression was evaluated by microarray, and where available, tissue typing 
      of mother/baby pairs was completed to determine HLA type. MxIF demonstrated 
      strong CD8+ T cell infiltration and HLA class I staining both the distal and stem 
      villi of VUE placentas. Compared to controls, VUE cases had significantly higher 
      expression of HLA class II mRNA and pathway analysis demonstrated that 40% of the 
      differentially expressed genes in VUE are related to tissue rejection. The data 
      suggest that VUE resembles a rejection response between the mother and the fetus. 
      It remains unknown what initiates immune recognition and why some mothers appear 
      to be at higher risk for developing this condition than others. Understanding 
      this etiology will be critical for developing effective interventions or 
      prevention strategies during pregnancy.
FAU - Enninga, Elizabeth Ann L
AU  - Enninga EAL
AD  - Department of Obstetrics and Gynecology, Mayo Clinic, 200 First Street SW, 
      Rochester, MN, 55905, USA. Enninga.elizabethann@mayo.edu.
FAU - Leontovich, Alexey A
AU  - Leontovich AA
AD  - Department of Health Science Research, Mayo Clinic, Rochester, MN, USA.
FAU - Fedyshyn, Bohdana
AU  - Fedyshyn B
AD  - Department of Obstetrics and Gynecology, Mayo Clinic, 200 First Street SW, 
      Rochester, MN, 55905, USA.
FAU - Wakefield, Laurie
AU  - Wakefield L
AD  - Department of Transfusion Medicine, Mayo Clinic, Rochester, MN, USA.
FAU - Gandhi, Manish
AU  - Gandhi M
AD  - Department of Transfusion Medicine, Mayo Clinic, Rochester, MN, USA.
FAU - Markovic, Svetomir N
AU  - Markovic SN
AD  - Department of Immunology, Mayo Clinic, Rochester, MN, USA.
FAU - Ruano, Rodrigo
AU  - Ruano R
AD  - Department of Obstetrics and Gynecology, Mayo Clinic, 200 First Street SW, 
      Rochester, MN, 55905, USA.
FAU - Kerr, Sarah E
AU  - Kerr SE
AD  - Department of Laboratory Pathology, Mayo Clinic, Rochester, MN, USA.
LA  - eng
GR  - K12 HD065987/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200106
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Chorionic Villi/*metabolism/pathology
MH  - Female
MH  - HLA Antigens/*metabolism
MH  - Humans
MH  - Inflammation/*metabolism/pathology
MH  - Lymphocytes/metabolism/pathology
MH  - Placenta/*metabolism/pathology
MH  - Placenta Diseases/*metabolism/pathology
MH  - Pregnancy
MH  - *Up-Regulation
PMC - PMC7255550
MID - NIHMS1548331
OTO - NOTNLM
OT  - HLA
OT  - Inflammation
OT  - Placenta
OT  - Rejection
OT  - Villitis
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2020/02/13 06:00
MHDA- 2020/12/16 06:00
PMCR- 2020/10/07
CRDT- 2020/02/13 06:00
PHST- 2019/05/23 00:00 [received]
PHST- 2019/09/17 00:00 [accepted]
PHST- 2020/02/13 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
PHST- 2020/02/13 06:00 [entrez]
PHST- 2020/10/07 00:00 [pmc-release]
AID - 10.1007/s43032-019-00101-9 [pii]
AID - 101 [pii]
AID - 10.1007/s43032-019-00101-9 [doi]
PST - ppublish
SO  - Reprod Sci. 2020 May;27(5):1129-1138. doi: 10.1007/s43032-019-00101-9. Epub 2020 
      Jan 6.