PMID- 32047813
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20201102
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2020
DP  - 2020
TI  - Identification of Key Modules, Hub Genes, and Noncoding RNAs in Chronic 
      Rhinosinusitis with Nasal Polyps by Weighted Gene Coexpression Network Analysis.
PG  - 6140728
LID - 10.1155/2020/6140728 [doi]
LID - 6140728
AB  - Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory 
      disease with relatively easy recurrence. However, the precise molecular 
      mechanisms of this disease are poorly known. Based on gene sequencing data 
      obtained from the Gene Expression Omnibus (GEO) database, we constructed 
      coexpression networks by weighted gene coexpression network analysis (WGCNA). 
      Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment 
      analyses were performed by the Database for Annotation, Visualization, and 
      Integrated Discovery (DAVID). The core gene of pathogenesis, CRSwNP, was screened 
      by protein-protein interaction data (PPI) from the HPRD database. Unsupervised 
      clustering was applied to screen hub genes related to the phenotype of CRSwNP. 
      Blue and turquoise modules were found to be most significantly related to the 
      pathogenicity of CRSwNP. Functional enrichment analysis showed that cell 
      proliferation in the blue modules, the apoptotic process in the turquoise module, 
      and the cancer pathway in both modules were mostly significantly correlated with 
      the development of CRSwNP. The noncoding RNAs (long noncoding RNA and microRNA) 
      and the top 10 core genes in each module were found to be associated with the 
      pathogenesis of CRSwNP. A total of nine hub genes were identified to be related 
      to the CRSwNP phenotype. By qRT-PCR analysis, AKT1, CDH1, PIK3R1, CBL, LRP1, 
      MALAT1, and XIST were proven to be associated with the pathogenesis of CRSwNP. 
      AGR2, FAM3D, PIP, DSE, and TMC were identified to be related to the CRSwNP 
      phenotype. Further exploration of these genes will reveal more important 
      information about the mechanisms of CRSwNP.
CI  - Copyright (c) 2020 Xuanchen Zhou et al.
FAU - Zhou, Xuanchen
AU  - Zhou X
AUID- ORCID: 0000-0002-2098-7266
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial 
      Hospital Affiliated to Shandong University, Jinan 250021, China.
FAU - Zhen, Xiaoyue
AU  - Zhen X
AD  - Minimally Invasive Urology Center, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan 250021, China.
FAU - Liu, Yiqing
AU  - Liu Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial 
      Hospital Affiliated to Shandong University, Jinan 250021, China.
FAU - Cui, Zhaoyang
AU  - Cui Z
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial 
      Hospital Affiliated to Shandong University, Jinan 250021, China.
FAU - Yue, Zhiyong
AU  - Yue Z
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial 
      Hospital Affiliated to Shandong University, Jinan 250021, China.
FAU - Xu, Anting
AU  - Xu A
AUID- ORCID: 0000-0003-3210-5731
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, The Second Hospital 
      of Shandong University, Jinan 250033, China.
FAU - Han, Jie
AU  - Han J
AUID- ORCID: 0000-0003-4868-984X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial 
      Hospital Affiliated to Shandong University, Jinan 250021, China.
LA  - eng
PT  - Journal Article
DEP - 20200123
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (AGR2 protein, human)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FAM3D protein, human)
RN  - 0 (LRP1 protein, human)
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-1)
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Mucoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (PIP protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Transcription Factors)
RN  - 0 (XIST non-coding RNA)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-cbl)
RN  - EC 2.7.1.- (PIK3R1 protein, human)
RN  - EC 2.7.1.137 (Class Ia Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (AKT1 protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 5.1.3.19 (DSE protein, human)
RN  - EC 6.3.2.- (CBL protein, human)
SB  - IM
MH  - Antigens, CD/genetics
MH  - Antigens, Neoplasm/genetics
MH  - Cadherins/genetics
MH  - Class Ia Phosphatidylinositol 3-Kinase/genetics
MH  - Cytokines/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Gene Expression Profiling
MH  - Gene Ontology
MH  - Gene Regulatory Networks/*genetics
MH  - Humans
MH  - Low Density Lipoprotein Receptor-Related Protein-1/genetics
MH  - Membrane Transport Proteins/genetics
MH  - Mucoproteins/genetics
MH  - Nasal Polyps/*genetics
MH  - Neoplasm Proteins/genetics
MH  - Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Proto-Oncogene Proteins c-cbl/genetics
MH  - RNA, Long Noncoding/genetics
MH  - RNA, Untranslated/*genetics
MH  - Sinusitis/*genetics
MH  - Transcription Factors/genetics
MH  - *Transcriptome
PMC - PMC7003281
COIS- The authors have no financial relationships and conflicts of interest to 
      disclose.
EDAT- 2020/02/13 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/01/23
CRDT- 2020/02/13 06:00
PHST- 2019/10/05 00:00 [received]
PHST- 2019/12/13 00:00 [revised]
PHST- 2019/12/20 00:00 [accepted]
PHST- 2020/02/13 06:00 [entrez]
PHST- 2020/02/13 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2020/01/23 00:00 [pmc-release]
AID - 10.1155/2020/6140728 [doi]
PST - epublish
SO  - Biomed Res Int. 2020 Jan 23;2020:6140728. doi: 10.1155/2020/6140728. eCollection 
      2020.