PMID- 32051086
OWN - NLM
STAT- MEDLINE
DCOM- 20200219
LR  - 20200808
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 22
IP  - 2
DP  - 2020 Feb
TI  - [Effect of Huai Qi Huang on asthma in rats and the mechanism research].
PG  - 171-176
AB  - OBJECTIVE: To study the role and mechanism of action of Huai Qi Huang (HQH) in 
      the rat model of asthma. METHODS: Forty Sprague-Dawley rats were randomly divided 
      into a control group, an asthma model group, a budesonide group, and an HQH 
      group, with 10 rats in each group. A rat model of asthma was established by 
      ovalbumin sensitization and challenge. The budesonide group was given budesonide 
      aerosol 2 mg before each challenge. The HQH group was given HQH 4 g/kg dissolved 
      in water by gavage before each challenge. Hematoxylin and eosin staining was used 
      to observe the pathological changes of lung tissues. The percentage of 
      eosinophils in bronchoalveolar lavage fluid (BALF) was measured. Enzyme-linked 
      immunosorbent assay was used to determine the levels of interleukin-3 (IL-3), 
      interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), interferon 
      gamma (INF-gamma), and immunoglobulin E (IgE) in BALF. Flow cytometry was used to 
      determine T-helper type 1 (Th1)/T-helper type 2 (Th2) ratio in peripheral blood 
      and the spleen. RT-PCR and Western blot were used to measure the mRNA and protein 
      expression of T-bet and GATA-3 in lung tissue. RESULTS: Compared with the control 
      group, the asthma model group showed significant increases in the degree of 
      airway inflammation, the percentage of eosinophils in BALF, and the levels of 
      IL-3, IL-4, IL-5 and IgE in BALF (P<0.05), however, the asthma model group showed 
      significant reductions in the levels of IL-10 and INF-gamma in BALF (P<0.05). The 
      asthma model group had significantly lower percentage of Th1 cells but 
      significantly higher percentage of Th2 cells in peripheral blood and the spleen 
      compared with the control group (P<0.05). The mRNA and protein expression of 
      T-bet in lung tissue was significantly lower, but the mRNA and protein expression 
      of GATA-3 in lung tissue was significantly higher in the asthma group than those 
      in the control group (P<0.05). Both HQH and budesonide significantly improved 
      airway inflammation and the above markers in asthmatic rats (P<0.05), with 
      comparable effects between them. However, there were still significant 
      differences in these indices between the control group and the HQH or budesonide 
      group (P<0.05). CONCLUSIONS: HQH can reduce the airway inflammation of asthmatic 
      rats and alleviate the symptoms of asthma, possibly by regulating the levels of 
      related cytokines and Th1/Th2 ratio through the T-bet/GATA-3 pathway.
FAU - Lu, Na
AU  - Lu N
AD  - Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China. yanbaixh@hust.edu.cn.
FAU - Bai, Yan
AU  - Bai Y
FAU - Zhao, Chen
AU  - Zhao C
FAU - Tian, Li
AU  - Tian L
FAU - Wan, Xia
AU  - Wan X
FAU - Jin, Run-Ming
AU  - Jin RM
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (huai qi huang)
SB  - IM
MH  - Animals
MH  - *Asthma
MH  - Bronchoalveolar Lavage Fluid
MH  - Drugs, Chinese Herbal
MH  - Lung
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Th2 Cells
PMC - PMC7390013
EDAT- 2020/02/14 06:00
MHDA- 2020/02/20 06:00
PMCR- 2020/02/25
CRDT- 2020/02/14 06:00
PHST- 2020/02/14 06:00 [entrez]
PHST- 2020/02/14 06:00 [pubmed]
PHST- 2020/02/20 06:00 [medline]
PHST- 2020/02/25 00:00 [pmc-release]
AID - 10.7499/j.issn.1008-8830.2020.02.016 [pii]
AID - zgddekzz-22-2-171 [pii]
AID - 10.7499/j.issn.1008-8830.2020.02.016 [doi]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2020 Feb;22(2):171-176. doi: 
      10.7499/j.issn.1008-8830.2020.02.016.