PMID- 32052065
OWN - NLM
STAT- MEDLINE
DCOM- 20200402
LR  - 20200402
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 412
IP  - 8
DP  - 2020 Mar
TI  - A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and 
      sensitive detection of specific IgE for mite allergens in sera from patients with 
      allergic rhinitis.
PG  - 1785-1794
LID - 10.1007/s00216-020-02422-0 [doi]
AB  - The prevalence of allergic rhinitis (AR) is increasing worldwide. However, the 
      current systems used to measure levels of immunoglobulin E (IgE) in sera are 
      associated with several disadvantages that limit their further application. 
      Consequently, there is a need to develop novel highly sensitive strategies that 
      can rapidly detect IgE in a quantitative manner. The development of such systems 
      will significantly enhance our ability to diagnose, treat, and even prevent AR. 
      Herein, we describe our experience of using quantum dot-based lateral flow 
      immunoassay (QD-LFIA), combined with a portable fluorescence immunoassay chip 
      detector (PFICD), to detect serum-specific IgE against Dermatophagoides 
      pteronyssinus (Der-p) and Dermatophagoides farinae (Der-f), two common mite 
      allergens in China. Our data showed that our system could detect serum-specific 
      levels of IgE against Der-p and Der-f as low as 0.093 IU/mL and 0.087 IU/mL, 
      respectively. We also established a standard curve to determine serum-specific 
      IgE concentrations that correlated well with the clinical BioIC microfluidics 
      system. The sensitivity of our assay was 96.7% for Der-p and 95.5% for Der-f, 
      while the specificity was 87.2% for Der-p and 85.3% for Der-f. Collectively, our 
      results demonstrate that QD-LFIA is a reliable system that could be applied to 
      detect serum-specific IgE in accordance with clinical demands. This QD-LFIA 
      strategy can be applied at home, in hospitals, and in pharmacies, with reduced 
      costs and time requirements when compared with existing techniques. In the 
      future, this system could be developed to detect other types of allergens and in 
      different types of samples (for example, whole blood). Graphical abstract We 
      describe our experiment using a quantum dot-based lateral flow immunoassay 
      combined with a portable fluorescence immunoassay chip detector for both 
      qualitative and quantitative detection of serum-specific IgE against two common 
      mite allergens. This strategy can be applied at home, in hospitals, and in 
      pharmacies, with reduced costs and time requirements. In the future, this system 
      could be developed to detect other types of allergens and in different types of 
      samples.
FAU - Liang, Zheng-Yan
AU  - Liang ZY
AD  - Department of Otolaryngology-Head and Neck Surgery, Central Laboratory, Renmin 
      Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, 430060, Hubei, China.
FAU - Deng, Yu-Qin
AU  - Deng YQ
AD  - Department of Otolaryngology-Head and Neck Surgery, Central Laboratory, Renmin 
      Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, 430060, Hubei, China.
FAU - Tao, Ze-Zhang
AU  - Tao ZZ
AD  - Department of Otolaryngology-Head and Neck Surgery, Central Laboratory, Renmin 
      Hospital of Wuhan University, 238 Jie-Fang Road, Wuhan, 430060, Hubei, China. 
      taozezhang11@163.com.
AD  - Institute of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, 238 Jie-Fang Road, Wuhan, 430060, Hubei, China. taozezhang11@163.com.
LA  - eng
GR  - NO.81670910/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200212
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*blood
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*blood
MH  - Limit of Detection
MH  - Point-of-Care Systems
MH  - *Quantum Dots
MH  - Rhinitis, Allergic/*blood/immunology
PMC - PMC7048869
OTO - NOTNLM
OT  - Allergic rhinitis
OT  - Immunoglobulin E
OT  - Lateral flow immunoassay
OT  - Quantum dots
COIS- The authors declare that they have no competing conflicts of interest.
EDAT- 2020/02/14 06:00
MHDA- 2020/04/03 06:00
PMCR- 2020/02/12
CRDT- 2020/02/14 06:00
PHST- 2019/11/11 00:00 [received]
PHST- 2020/01/14 00:00 [accepted]
PHST- 2020/01/08 00:00 [revised]
PHST- 2020/02/14 06:00 [pubmed]
PHST- 2020/04/03 06:00 [medline]
PHST- 2020/02/14 06:00 [entrez]
PHST- 2020/02/12 00:00 [pmc-release]
AID - 10.1007/s00216-020-02422-0 [pii]
AID - 2422 [pii]
AID - 10.1007/s00216-020-02422-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2020 Mar;412(8):1785-1794. doi: 10.1007/s00216-020-02422-0. 
      Epub 2020 Feb 12.