PMID- 32058052
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 1873-6971 (Electronic)
IS  - 0367-326X (Linking)
VI  - 144
DP  - 2020 Jul
TI  - Tussilagone inhibits allergic responses in OVA-induced allergic rhinitis guinea 
      pigs and IgE-stimulated RBL-2H3 cells.
PG  - 104496
LID - S0367-326X(20)30078-2 [pii]
LID - 10.1016/j.fitote.2020.104496 [doi]
AB  - Farfarae Flos is the dried flower buds of Tussilago farfara L. which is widely 
      used to treat allergic and inflammatory diseases in Chinese folk. Tussilagone 
      (TSL), a sesquiterpene compound purified from Farfarae Flos, has been confirmed 
      the main active component in the plant. However, its anti-allergic activity 
      hasn't been reported yet. The purpose of this study is to investigate the 
      anti-allergic effect of TSL in ovalbumin (OVA)-induced allergic rhinitis (AR) 
      guinea pigs and immunoglobulin E (IgE)-stimulated RBL-2H3 cells. The AR symptoms 
      such as nasal scratching, sneezing and runny nose were scored and the 
      histological changes of nasal mucosa were observed by H&E staining. The levels of 
      histamine, OVA-specific IgE, IL-6 and TNF-alpha in the serum were measured by ELISA. 
      In IgE-stimulated RBL-2H3 cells, the phosphoryration of Lyn, Syk, Akt, NF-kappaB p65, 
      ERK and p38 MAPK were investigated by western blot analysis. The results showed 
      that intraperitoneal injection of TSL at doses of 25 and 50 mg/kg significantly 
      alleviated the allergic symptoms and the histological changes of nasal mucosa in 
      OVA-induced allergic rhinitis guinea pigs. Moreover, the levels of histamine, IgE 
      and IL-6 in the serum decreased significantly (p < .05). In vitro, TSL suppressed 
      the phosphorylation of Lyn, Syk, Akt, NF-kappaB p65, ERK and p38 MAPK in 
      IgE-stimulated RBL-2H3 cells. These results indicate TSL has therapeutic effect 
      on allergic rhinitis in guinea pigs. The anti-allergic mechanism may be through 
      the inhibition of allergic and inflammatory related pathways in mast cells.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Jin, Chaoying
AU  - Jin C
AD  - School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 
      310053, Zhejiang, China.
FAU - Ye, Kang
AU  - Ye K
AD  - Zhejiang Research Institute of Chinese Medicine Co., Ltd, Hangzhou 310053, 
      Zhejiang, China.
FAU - Luan, Huiling
AU  - Luan H
AD  - Zhejiang Research Institute of Chinese Medicine Co., Ltd, Hangzhou 310053, 
      Zhejiang, China.
FAU - Liu, Liying
AU  - Liu L
AD  - School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 
      310053, Zhejiang, China.
FAU - Zhang, Rusong
AU  - Zhang R
AD  - Zhejiang Research Institute of Chinese Medicine Co., Ltd, Hangzhou 310053, 
      Zhejiang, China.
FAU - Yang, Subei
AU  - Yang S
AD  - Zhejiang Research Institute of Chinese Medicine Co., Ltd, Hangzhou 310053, 
      Zhejiang, China. Electronic address: 13805782590@qq.com.
FAU - Wang, Yiqi
AU  - Wang Y
AD  - School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 
      310053, Zhejiang, China. Electronic address: wangyiqi2011@126.com.
LA  - eng
PT  - Journal Article
DEP - 20200210
PL  - Netherlands
TA  - Fitoterapia
JT  - Fitoterapia
JID - 16930290R
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-6)
RN  - 0 (Phytochemicals)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - 9006-59-1 (Ovalbumin)
RN  - 95N0GBB2SX (tussilagone)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*therapeutic use
MH  - Cell Line
MH  - Drugs, Chinese Herbal/therapeutic use
MH  - Female
MH  - Guinea Pigs
MH  - Histamine/blood
MH  - Immunoglobulin E/blood/pharmacology
MH  - Interleukin-6/blood
MH  - Male
MH  - Molecular Structure
MH  - Nasal Mucosa/*drug effects/pathology
MH  - Ovalbumin
MH  - Phytochemicals/therapeutic use
MH  - Random Allocation
MH  - Rhinitis, Allergic/chemically induced/*drug therapy
MH  - Sesquiterpenes/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/blood
OTO - NOTNLM
OT  - Allergic rhinitis
OT  - Ovalbumin
OT  - RBL-2H3 cell
OT  - Tussilagone
EDAT- 2020/02/15 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/02/15 06:00
PHST- 2019/12/04 00:00 [received]
PHST- 2020/02/05 00:00 [revised]
PHST- 2020/02/07 00:00 [accepted]
PHST- 2020/02/15 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PHST- 2020/02/15 06:00 [entrez]
AID - S0367-326X(20)30078-2 [pii]
AID - 10.1016/j.fitote.2020.104496 [doi]
PST - ppublish
SO  - Fitoterapia. 2020 Jul;144:104496. doi: 10.1016/j.fitote.2020.104496. Epub 2020 
      Feb 10.