PMID- 32064751 OWN - NLM STAT- MEDLINE DCOM- 20210514 LR - 20231111 IS - 1751-7915 (Electronic) IS - 1751-7915 (Linking) VI - 13 IP - 4 DP - 2020 Jul TI - Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineered Synechococcus elongatus and Pseudomonas putida. PG - 997-1011 LID - 10.1111/1751-7915.13544 [doi] AB - In contrast to the current paradigm of using microbial mono-cultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed-species consortia to perform functions that are difficult to programme into individual strains. In this work, we developed a synthetic microbial consortium composed of two genetically engineered microbes, a cyanobacterium (Synechococcus elongatus PCC 7942) and a heterotrophic bacterium (Pseudomonas putida EM173). These microbial species specialize in the co-culture: cyanobacteria fix CO(2) through photosynthetic metabolism and secrete sufficient carbohydrates to support the growth and active metabolism of P. putida, which has been engineered to consume sucrose and to degrade the environmental pollutant 2,4-dinitrotoluene (2,4-DNT). By encapsulating S. elongatus within a barium-alginate hydrogel, cyanobacterial cells were protected from the toxic effects of 2,4-DNT, enhancing the performance of the co-culture. The synthetic consortium was able to convert 2,4-DNT with light and CO(2) as key inputs, and its catalytic performance was stable over time. Furthermore, cycling this synthetic consortium through low nitrogen medium promoted the sucrose-dependent accumulation of polyhydroxyalkanoate, an added-value biopolymer, in the engineered P. putida strain. Altogether, the synthetic consortium displayed the capacity to remediate the industrial pollutant 2,4-DNT while simultaneously synthesizing biopolymers using light and CO(2) as the primary inputs. CI - (c) 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. FAU - Fedeson, Derek T AU - Fedeson DT AD - DOE-MSU Plant Research Laboratories, Michigan State University, East Lansing, MI, USA. AD - Genetics Program, Michigan State University, East Lansing, MI, USA. FAU - Saake, Pia AU - Saake P AD - Heinrich-Heine Universitat, Dusseldorf, Germany. FAU - Calero, Patricia AU - Calero P AD - The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, Denmark. FAU - Nikel, Pablo Ivan AU - Nikel PI AUID- ORCID: 0000-0002-9313-7481 AD - The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs Lyngby, Denmark. FAU - Ducat, Daniel C AU - Ducat DC AUID- ORCID: 0000-0002-1520-0588 AD - DOE-MSU Plant Research Laboratories, Michigan State University, East Lansing, MI, USA. AD - Genetics Program, Michigan State University, East Lansing, MI, USA. AD - Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20200216 PL - United States TA - Microb Biotechnol JT - Microbial biotechnology JID - 101316335 RN - 0 (Dinitrobenzenes) RN - 6741D310ED (2,4-dinitrotoluene) RN - Synechococcus elongatus SB - IM MH - Biotransformation MH - Coculture Techniques MH - Dinitrobenzenes MH - *Pseudomonas putida/genetics MH - Synechococcus PMC - PMC7264894 COIS- None declared. EDAT- 2020/02/18 06:00 MHDA- 2021/05/15 06:00 PMCR- 2020/02/16 CRDT- 2020/02/18 06:00 PHST- 2019/02/25 00:00 [received] PHST- 2020/01/27 00:00 [revised] PHST- 2020/01/28 00:00 [accepted] PHST- 2020/02/18 06:00 [pubmed] PHST- 2021/05/15 06:00 [medline] PHST- 2020/02/18 06:00 [entrez] PHST- 2020/02/16 00:00 [pmc-release] AID - MBT213544 [pii] AID - 10.1111/1751-7915.13544 [doi] PST - ppublish SO - Microb Biotechnol. 2020 Jul;13(4):997-1011. doi: 10.1111/1751-7915.13544. Epub 2020 Feb 16.