PMID- 32065300
OWN - NLM
STAT- MEDLINE
DCOM- 20200918
LR  - 20200918
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 35
IP  - 7
DP  - 2020 Sep
TI  - Attenuation of the inflammatory response and polarization of macrophages by 
      photobiomodulation.
PG  - 1509-1518
LID - 10.1007/s10103-019-02941-y [doi]
AB  - In spinal cord injury (SCI), inflammation is a major mediator of damage and loss 
      of function and is regulated primarily by the bone marrow-derived macrophages 
      (BMDMs). Photobiomodulation (PBM) or low-level light stimulation is known to have 
      anti-inflammatory effects and has previously been used in the treatment of SCI, 
      although its precise cellular mechanisms remain unclear. In the present study, 
      the effect of PBM at 810 nm on classically activated BMDMs was evaluated to 
      investigate the mechanisms underlying its anti-inflammatory effects. BMDMs were 
      cultured and irradiated (810 nm, 2 mW/cm(2)) following stimulation with 
      lipopolysaccharide and interferon-gamma. CCK-8 assay, 2',7'-dichlorofluorescein 
      diacetate assay, and ELISA and western blot analysis were performed to measure 
      cell viability, reactive oxygen species production, and inflammatory marker 
      production, respectively. PBM irradiation of classically activated macrophages 
      significantly increased the cell viability and inhibited reactive oxygen species 
      generation. PBM suppressed the expression of a marker of classically activated 
      macrophages, inducible nitric oxide synthase; decreased the mRNA expression and 
      secretion of pro-inflammatory cytokines, tumor necrosis factor alpha, and 
      interleukin-1 beta; and increased the secretion of monocyte chemotactic protein 
      1. Exposure to PBM likewise significantly reduced the expression and 
      phosphorylation of NF-kappaB p65 in classically activated BMDMs. Taken together, 
      these results suggest that PBM can successfully modulate inflammation and 
      polarization in classically activated BMDMs. The present study provides a 
      theoretical basis to support wider clinical application of PBM in the treatment 
      of SCI.
FAU - Li, Kun
AU  - Li K
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Liang, Zhuowen
AU  - Liang Z
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Zhang, Jiawei
AU  - Zhang J
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Zuo, Xiaoshuang
AU  - Zuo X
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Sun, Jiakai
AU  - Sun J
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Zheng, Qiao
AU  - Zheng Q
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Song, Jiwei
AU  - Song J
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Ding, Tan
AU  - Ding T
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China.
FAU - Hu, Xueyu
AU  - Hu X
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China. huxueyu@fmmu.edu.cn.
FAU - Wang, Zhe
AU  - Wang Z
AD  - Xijing Orthopaedics Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 
      China. wangzhe@fmmu.edu.cn.
LA  - eng
GR  - 81572151/National Natural Science Foundation of China/
GR  - 2016SF-143/Key Science and Technology Program in Social Development of Shaanxi 
      Province/
PT  - Journal Article
DEP - 20200217
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factor RelA)
SB  - IM
MH  - Animals
MH  - *Cell Polarity/radiation effects
MH  - Cell Survival/radiation effects
MH  - Chemokines/genetics/metabolism
MH  - Gene Expression Regulation/radiation effects
MH  - Inflammation/*radiotherapy
MH  - Macrophage Activation/radiation effects
MH  - Macrophages/*pathology/radiation effects
MH  - Mice, Inbred BALB C
MH  - Phosphorylation/radiation effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Transcription Factor RelA/metabolism
OTO - NOTNLM
OT  - Inflammation
OT  - Macrophage
OT  - Photobiomodulation
OT  - Polarization
OT  - Spinal cord injury
EDAT- 2020/02/18 06:00
MHDA- 2020/09/20 06:00
CRDT- 2020/02/18 06:00
PHST- 2019/02/13 00:00 [received]
PHST- 2019/12/22 00:00 [accepted]
PHST- 2020/02/18 06:00 [pubmed]
PHST- 2020/09/20 06:00 [medline]
PHST- 2020/02/18 06:00 [entrez]
AID - 10.1007/s10103-019-02941-y [pii]
AID - 10.1007/s10103-019-02941-y [doi]
PST - ppublish
SO  - Lasers Med Sci. 2020 Sep;35(7):1509-1518. doi: 10.1007/s10103-019-02941-y. Epub 
      2020 Feb 17.