PMID- 32065407 OWN - NLM STAT- MEDLINE DCOM- 20210121 LR - 20210121 IS - 1531-4995 (Electronic) IS - 0023-852X (Print) IS - 0023-852X (Linking) VI - 130 IP - 12 DP - 2020 Dec TI - Distinct Biomarker Profiles and Clinical Characteristics in T1-T2 Glottic and Supraglottic Carcinomas. PG - 2825-2832 LID - 10.1002/lary.28532 [doi] AB - BACKGROUND: In early stage laryngeal squamous cell carcinoma (LSCC) radiotherapy with curative intent is a major treatment modality. TNM classification is used to define patients eligible for radiotherapy. Studies in early stage glottic LSCC identified several predictive biomarkers associated with local control. However, we recently reported that this predictive value could not be confirmed in supraglottic LSCC. OBJECTIVE: To examine whether clinical behavior and protein expression patterns of these biomarkers differ between glottic and supraglottic LSCC. STUDY DESIGN: Retrospective cohort study. METHODS: Tumor tissue sections of 196 glottic and 80 supraglottic T1-T2 LSCC treated primarily with RT were assessed immunohistochemically for expression of pAKT, Ki-67 and beta-Catenin. Expression data of HIF-1alpha, CA-IX, OPN, FADD, pFADD, Cyclin D1, Cortactin and EGFR in the same cohort of glottic and supraglottic LSCC, were retrieved from previously reported data. The relationship between glottic and supraglottic sublocalization and clinicopathological, follow-up, and immunohistochemical staining characteristics were evaluated using logistic regression and Cox regression analyses. RESULTS: Glottic LSCC were correlated with male gender (P = .001), hoarseness as a primary symptom (P < .001), T1 tumor stage (P < .001), negative lymph node status (P < .001), and an older age at presentation (P = .004). Supraglottic LSCC patients developed more post-treatment distant metastasis when adjusted for gender, age, and T-status. While supraglottic LSCC was associated with higher expression of HIF-1alpha (P = .001), Cortactin (P < .001), EGFR (P < .001), and Ki-67 (P = .027), glottic LSCC demonstrated higher expression of CA-IX (P = .005) and Cyclin D1 (P = .001). CONCLUSION: Differences in clinicopathological and immunohistochemical staining characteristics suggest that T1-T2 glottic and supraglottic LSCC should be considered as different entities. LEVEL OF EVIDENCE: N/A. Laryngoscope, 2020. CI - (c) 2020 The Authors. The Laryngoscope published by Wiley Periodicals, Inc. on behalf of The American Laryngological, Rhinological and Otological Society, Inc. FAU - Wachters, Jan E AU - Wachters JE AUID- ORCID: 0000-0001-5844-0613 AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. AD - Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - Kop, Emiel AU - Kop E AUID- ORCID: 0000-0001-5731-4439 AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. AD - Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - Slagter-Menkema, Lorian AU - Slagter-Menkema L AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. AD - Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - Mastik, Mirjam AU - Mastik M AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - van der Wal, Jacqueline E AU - van der Wal JE AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - van der Vegt, Bert AU - van der Vegt B AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - de Bock, Geertruida H AU - de Bock GH AD - Department of Epidemiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - van der Laan, Bernard F A M AU - van der Laan BFAM AD - Department of Otorhinolaryngology and Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. FAU - Schuuring, Ed AU - Schuuring E AD - Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200217 PL - United States TA - Laryngoscope JT - The Laryngoscope JID - 8607378 RN - 0 (Biomarkers, Tumor) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Biomarkers, Tumor/*metabolism MH - Carcinoma, Squamous Cell/*pathology/radiotherapy MH - Female MH - Glottis/*pathology MH - Humans MH - Immunohistochemistry MH - Laryngeal Neoplasms/*pathology/radiotherapy MH - Male MH - Middle Aged MH - Neoplasm Metastasis MH - Neoplasm Staging MH - Retrospective Studies MH - Sex Factors PMC - PMC7754398 OTO - NOTNLM OT - Biomarkers OT - glottic laryngeal squamous cell carcinoma OT - head and neck squamous cell carcinoma OT - supraglottic laryngeal squamous cell carcinoma EDAT- 2020/02/18 06:00 MHDA- 2021/01/22 06:00 PMCR- 2020/12/22 CRDT- 2020/02/18 06:00 PHST- 2019/09/30 00:00 [received] PHST- 2019/12/05 00:00 [revised] PHST- 2019/12/31 00:00 [accepted] PHST- 2020/02/18 06:00 [pubmed] PHST- 2021/01/22 06:00 [medline] PHST- 2020/02/18 06:00 [entrez] PHST- 2020/12/22 00:00 [pmc-release] AID - LARY28532 [pii] AID - 10.1002/lary.28532 [doi] PST - ppublish SO - Laryngoscope. 2020 Dec;130(12):2825-2832. doi: 10.1002/lary.28532. Epub 2020 Feb 17.