PMID- 32069796
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 9
IP  - 2
DP  - 2020 Feb 13
TI  - Homologous Proteins of the Manganese Transporter PAM71 Are Localized in the Golgi 
      Apparatus and Endoplasmic Reticulum.
LID - 10.3390/plants9020239 [doi]
LID - 239
AB  - Chloroplast manganese transporter 1 (CMT1) and photosynthesis-affected mutant 71 
      (PAM71) are two membrane proteins that function sequentially to mediate the 
      passage of manganese across the chloroplast envelope and the thylakoid membrane. 
      CMT1 and PAM71 belong to a small five-member protein family in Arabidopsis 
      thaliana. The other three, photosynthesis-affected mutant 71 like 3 (PML3), PML4 
      and PML5 are not predicted to reside in chloroplast membranes. In this study, the 
      subcellular localization of PML3:GFP, PML4:GFP and PML5:GFP was determined using 
      transient and stable expression assays. PML3:GFP localizes to the Golgi 
      apparatus, whereas PML4:GFP and PML5:GFP are found in the endoplasmic reticulum. 
      We also examined patterns of PML3, PML4 and PML5 promoter activity. Although the 
      precise expression pattern of each promoter was unique, all three genes were 
      expressed in the leaf vasculature and in roots. Greenhouse grown single mutants 
      pml3, pml4, pml5 and the pml4/pml5 double mutant did not exhibit growth defects, 
      however an inspection of the root growth revealed a difference between pml3 and 
      the other genotypes, including wild-type, in 500 microM manganese growth conditions. 
      Strikingly, overexpression of PML3 resulted in a stunted growth phenotype. 
      Putative functions of PML3, PML4 and PML5 are discussed in light of what is known 
      about PAM71 and CMT1.
FAU - Hoecker, Natalie
AU  - Hoecker N
AD  - Molekularbiologie der Pflanze (Botanik), Department Biologie I, 
      Ludwig-Maximilians-Universitat Munchen, 82152 Martinsried, Germany.
FAU - Honke, Anna
AU  - Honke A
AD  - Molekularbiologie der Pflanze (Botanik), Department Biologie I, 
      Ludwig-Maximilians-Universitat Munchen, 82152 Martinsried, Germany.
FAU - Frey, Katharina
AU  - Frey K
AUID- ORCID: 0000-0001-8060-5736
AD  - Molekularbiologie der Pflanze (Botanik), Department Biologie I, 
      Ludwig-Maximilians-Universitat Munchen, 82152 Martinsried, Germany.
FAU - Leister, Dario
AU  - Leister D
AUID- ORCID: 0000-0003-1897-8421
AD  - Molekularbiologie der Pflanze (Botanik), Department Biologie I, 
      Ludwig-Maximilians-Universitat Munchen, 82152 Martinsried, Germany.
FAU - Schneider, Anja
AU  - Schneider A
AUID- ORCID: 0000-0002-3377-5828
AD  - Molekularbiologie der Pflanze (Botanik), Department Biologie I, 
      Ludwig-Maximilians-Universitat Munchen, 82152 Martinsried, Germany.
LA  - eng
GR  - SCHN 560/4-1/DFG German Science Foundation/
GR  - SCHN 560/4-1/DFG German Science Foundation/
PT  - Journal Article
DEP - 20200213
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC7076475
OTO - NOTNLM
OT  - Arabidopsis
OT  - CMT1
OT  - PAM71
OT  - UPF0016 protein family
OT  - manganese transporter
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/20 06:00
MHDA- 2020/02/20 06:01
PMCR- 2020/02/13
CRDT- 2020/02/20 06:00
PHST- 2019/12/13 00:00 [received]
PHST- 2020/02/10 00:00 [revised]
PHST- 2020/02/11 00:00 [accepted]
PHST- 2020/02/20 06:00 [entrez]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2020/02/20 06:01 [medline]
PHST- 2020/02/13 00:00 [pmc-release]
AID - plants9020239 [pii]
AID - plants-09-00239 [pii]
AID - 10.3390/plants9020239 [doi]
PST - epublish
SO  - Plants (Basel). 2020 Feb 13;9(2):239. doi: 10.3390/plants9020239.