PMID- 32072235
OWN - NLM
STAT- MEDLINE
DCOM- 20200921
LR  - 20200921
IS  - 1437-9813 (Electronic)
IS  - 0179-0358 (Linking)
VI  - 36
IP  - 4
DP  - 2020 Apr
TI  - Effect of unilateral testicular torsion on contralateral testis in a rat model.
PG  - 529-536
LID - 10.1007/s00383-020-04626-y [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the function of the 
      contralateral testis after unilateral testicular torsion (UTT) and its possible 
      mechanism. METHODS: 56 rats were randomly divided into four groups: Group A: Sham 
      operation, Group B: Testicular torsion (TT)+normal saline (NS), Group C: 
      Testicular torsion (TT)+cyclosporine, Group D: Testicular torsion 
      (TT)+NG-Monomethyl-L-arginine (L-NMMA). The right testes were removed 1 week and 
      8 weeks after surgery, respectively. Biochemistry and histopathologic evaluations 
      were used to evaluate the germ cell damage. RESULTS: Compared with Group A, the 
      levels of malondialchehyche (MDA) and nitric oxide (NO)/nitricoxide synthase 
      (NOS) were increased remarkably in Group B. Significant differences were shown 
      between histopathological damages and density and motility of sperm in two 
      groups. Compared with Group B, the levels of MDA and NO/NOS in Group D decreased 
      significantly while mean seminiferous tubule diameter (MSTD) and mean testicular 
      biopsy scoring (MTBS) maintained in a better condition. The levels of major 
      histocompatibility complex (MHC) peptide-tetramer complex in Group C and Group D 
      decreased significantly than Group B, while sperm density and motility were 
      significantly higher than Group B. It was also known that the histopathological 
      damages in Group C and Group D were less than those in Group B in the 8 weeks 
      after operation. CONCLUSION: UTT can cause impairment of contralateral testicular 
      function and decrease of spermatogenic function. The mechanism may be related to 
      ischemia-reperfusion (IR) in early stage and autoimmune response in late stage.
FAU - Xia, Zhaobo
AU  - Xia Z
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, 
      China.
FAU - Hu, Jun
AU  - Hu J
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, 
      China.
FAU - Han, Lei
AU  - Han L
AD  - Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming 
      Medical University, Kunming, 650101, Yunnan, China.
FAU - Xia, Qiongzhang
AU  - Xia Q
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, 
      China.
FAU - Shao, Fangbin
AU  - Shao F
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, 
      China.
FAU - Lin, Xiaokun
AU  - Lin X
AUID- ORCID: 0000-0002-9347-9860
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, Zhejiang, 
      China. linxk2000@163.com.
LA  - eng
GR  - Y20190085/Wenzhou Municipal Science and Technology Bureau/
GR  - 2020KY645/Zhejiang Medical and Health Science Project/
PT  - Journal Article
DEP - 20200218
PL  - Germany
TA  - Pediatr Surg Int
JT  - Pediatric surgery international
JID - 8609169
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Germ Cells/pathology
MH  - Humans
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/diagnosis/*etiology/physiopathology
MH  - Seminiferous Tubules/*pathology
MH  - Spermatic Cord Torsion/*complications/diagnosis/physiopathology
MH  - Spermatogenesis/*physiology
MH  - Testis/metabolism/*pathology/physiopathology
OTO - NOTNLM
OT  - Autoimmune response
OT  - Contralateral
OT  - Ischemia-reperfusion
OT  - Testicular torsion
EDAT- 2020/02/20 06:00
MHDA- 2020/09/22 06:00
CRDT- 2020/02/20 06:00
PHST- 2020/02/03 00:00 [accepted]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2020/09/22 06:00 [medline]
PHST- 2020/02/20 06:00 [entrez]
AID - 10.1007/s00383-020-04626-y [pii]
AID - 10.1007/s00383-020-04626-y [doi]
PST - ppublish
SO  - Pediatr Surg Int. 2020 Apr;36(4):529-536. doi: 10.1007/s00383-020-04626-y. Epub 
      2020 Feb 18.