PMID- 32072449
OWN - NLM
STAT- MEDLINE
DCOM- 20201228
LR  - 20201228
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 61
IP  - 2
DP  - 2020 May
TI  - Development, identification, and characterization of blue-grained wheat-Triticum 
      boeoticum substitution lines.
PG  - 169-177
LID - 10.1007/s13353-020-00553-9 [doi]
AB  - Diploid wild einkorn wheat, Triticum boeoticum Boiss (A(b)A(b), 2n = 2x = 14), is 
      a wheat-related species with a blue aleurone layer. In this study, six 
      blue-grained wheat lines were developed from F(8) progeny of crosses between 
      common wheat and T. boeoticum. The chromosome constitutions of these lines were 
      characterized by fluorescence in situ hybridization (FISH) using the 
      oligonucleotide probes Oligo-pTa535-1, Oligo-pSc119.2-1, Oligo-pTa71-2, and 
      (AAC)(7). Multicolor FISH using Oligo-pTa535-1, Oligo-pSc119.2-1, and 
      Oligo-pTa71-2 identified all 42 common wheat chromosomes, while Oligo-pTa535-1 
      and (AAC)(7) discriminated the 14 chromosomes of T. boeoticum. FISH revealed that 
      all six blue-grained lines were wheat-T. boeoticum 4A(b) (4B) disomic 
      substitution lines. The substitution lines were validated by genotyping using the 
      wheat 55 K single nucleotide polymorphism (SNP) array containing 53,063 markers. 
      These 4A(b) (4B) substitution lines represent novel germplasm for blue-grained 
      wheat breeding. The FISH probes and SNP markers used here can be applied in the 
      development of blue-grained wheat-Triticum boeoticum translocation lines.
FAU - Liu, Xin
AU  - Liu X
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Feng, Zhen
AU  - Feng Z
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Liang, Dongyu
AU  - Liang D
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Zhang, Minghu
AU  - Zhang M
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Liu, Xiaojuan
AU  - Liu X
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Hao, Ming
AU  - Hao M
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Liu, Dengcai
AU  - Liu D
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Ning, Shunzong
AU  - Ning S
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Yuan, Zhongwei
AU  - Yuan Z
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Jiang, Bo
AU  - Jiang B
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Chen, Xuejiao
AU  - Chen X
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Chen, Xue
AU  - Chen X
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China.
FAU - Zhang, Lianquan
AU  - Zhang L
AUID- ORCID: 0000-0001-9005-8705
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. 
      zhanglianquan1977@126.com.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, China. zhanglianquan1977@126.com.
LA  - eng
GR  - 31671689/the National Natural Science Foundation of China/
GR  - 31671682/the National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200218
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (Oligonucleotides)
RN  - 0 (Plant Proteins)
RN  - 0 (aleurone)
SB  - IM
MH  - Breeding
MH  - Chromosomes, Plant/*genetics
MH  - Crosses, Genetic
MH  - Diploidy
MH  - Edible Grain/*genetics/growth & development
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotides/genetics
MH  - Plant Proteins/*genetics
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Triticum/*genetics/growth & development
OTO - NOTNLM
OT  - Blue aleurone
OT  - Fluorescence in situ hybridization
OT  - Single nucleotide polymorphism
OT  - Substitution line
OT  - Triticum boeoticum
EDAT- 2020/02/20 06:00
MHDA- 2020/12/29 06:00
CRDT- 2020/02/20 06:00
PHST- 2019/11/13 00:00 [received]
PHST- 2020/02/12 00:00 [accepted]
PHST- 2020/02/06 00:00 [revised]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2020/02/20 06:00 [entrez]
AID - 10.1007/s13353-020-00553-9 [pii]
AID - 10.1007/s13353-020-00553-9 [doi]
PST - ppublish
SO  - J Appl Genet. 2020 May;61(2):169-177. doi: 10.1007/s13353-020-00553-9. Epub 2020 
      Feb 18.