PMID- 32073701 OWN - NLM STAT- MEDLINE DCOM- 20210304 LR - 20210304 IS - 1365-313X (Electronic) IS - 0960-7412 (Print) IS - 0960-7412 (Linking) VI - 103 IP - 1 DP - 2020 Jul TI - Metabolomics analysis and metabolite-agronomic trait associations using kernels of wheat (Triticum aestivum) recombinant inbred lines. PG - 279-292 LID - 10.1111/tpj.14727 [doi] AB - Plants produce numerous metabolites that are important for their development and growth. However, the genetic architecture of the wheat metabolome has not been well studied. Here, utilizing a high-density genetic map, we conducted a comprehensive metabolome study via widely targeted LC-MS/MS to analyze the wheat kernel metabolism. We further combined agronomic traits and dissected the genetic relationship between metabolites and agronomic traits. In total, 1260 metabolic features were detected. Using linkage analysis, 1005 metabolic quantitative trait loci (mQTLs) were found distributed unevenly across the genome. Twenty-four candidate genes were found to modulate the levels of different metabolites, of which two were functionally annotated by in vitro analysis to be involved in the synthesis and modification of flavonoids. Combining the correlation analysis of metabolite-agronomic traits with the co-localization of methylation quantitative trait locus (mQTL) and phenotypic QTL (pQTL), genetic relationships between the metabolites and agronomic traits were uncovered. For example, a candidate was identified using correlation and co-localization analysis that may manage auxin accumulation, thereby affecting number of grains per spike (NGPS). Furthermore, metabolomics data were used to predict the performance of wheat agronomic traits, with metabolites being found that provide strong predictive power for NGPS and plant height. This study used metabolomics and association analysis to better understand the genetic basis of the wheat metabolism which will ultimately assist in wheat breeding. CI - (c) 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. FAU - Shi, Taotao AU - Shi T AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Zhu, Anting AU - Zhu A AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Jia, Jingqi AU - Jia J AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Hu, Xin AU - Hu X AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Chen, Jie AU - Chen J AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Liu, Wei AU - Liu W AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Ren, Xifeng AU - Ren X AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Sun, Dongfa AU - Sun D AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Fernie, Alisdair R AU - Fernie AR AUID- ORCID: 0000-0001-9000-335X AD - Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, 14476, Germany. FAU - Cui, Fa AU - Cui F AD - Wheat Molecular Breeding Innovation Research Group, Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong, School of Agriculture, Ludong University, Yantai, China. FAU - Chen, Wei AU - Chen W AUID- ORCID: 0000-0001-7225-3785 AD - National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. AD - College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200331 PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 SB - IM MH - Edible Grain/*metabolism MH - Genes, Plant/genetics MH - Genetic Association Studies MH - Metabolomics MH - Plant Breeding/methods MH - Polymorphism, Single Nucleotide/genetics MH - Quantitative Trait Loci/genetics MH - *Quantitative Trait, Heritable MH - Triticum/genetics/*metabolism PMC - PMC7383920 OTO - NOTNLM OT - Triticum aestivum L. OT - agronomic trait OT - mature seed OT - metabolic prediction OT - metabolic quantitative trait loci COIS- The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted. EDAT- 2020/02/20 06:00 MHDA- 2021/03/05 06:00 PMCR- 2020/07/27 CRDT- 2020/02/20 06:00 PHST- 2019/09/30 00:00 [received] PHST- 2020/01/17 00:00 [revised] PHST- 2020/02/07 00:00 [accepted] PHST- 2020/02/20 06:00 [pubmed] PHST- 2021/03/05 06:00 [medline] PHST- 2020/02/20 06:00 [entrez] PHST- 2020/07/27 00:00 [pmc-release] AID - TPJ14727 [pii] AID - 10.1111/tpj.14727 [doi] PST - ppublish SO - Plant J. 2020 Jul;103(1):279-292. doi: 10.1111/tpj.14727. Epub 2020 Mar 31.