PMID- 32074495
OWN - NLM
STAT- MEDLINE
DCOM- 20210126
LR  - 20210126
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 147
DP  - 2020 Apr 15
TI  - Expression of genes involved in the NF-kappaB-dependent pathway of the fibrosis in 
      the mare endometrium.
PG  - 18-24
LID - S0093-691X(20)30068-6 [pii]
LID - 10.1016/j.theriogenology.2020.01.055 [doi]
AB  - Equine endometrosis is a multifactorial chronic degenerative condition, 
      considered to be one of a major causes of equine infertility. The formation of 
      periglandular fibrosis seems to be linked to chronic inflammation of the mare 
      endometrium in a paracrine way and in a response to numerous forms of 
      inflammatory stimuli elicit the net deposition of extracellular matrix (ECM) 
      around the endometrial glands and stroma. We hypothesized some of these stimuli, 
      such as monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), and 
      hyaluronan synthases (HASs), may share the nuclear factor-kappaB (NF-kappaB) dependent 
      activation pathway. This study aimed to determine whether mRNA expression of 
      MCP-1, IL-6, HASs, and proteins of canonical (RelA/NK-kappabeta1) and noncanonical 
      (NK-kappabeta2) signaling pathways for NF-kB would change in subsequent categories of 
      endometrosis during the estrous cycle. The expression of selected genes was 
      established in mare endometrium (n = 80; Kenney and Doig categories I, IIA, IIB, 
      III), obtained in the follicular phase (FLP) and mid-luteal phase (MLP). The high 
      expression of RelA mRNA was observed in III, whereas of NK-kappabeta1 and NK-kappabeta2 also in 
      IIA, and IIA and IIB, respectively. The expression of MCP-1 mRNA occurred 
      constantly, regardless of the category, whereas IL-6 mRNA was low in IIA, IIB, 
      and III. The expression of HAS 1 was high in IIA and HAS 3 in IIA, IIB, and III. 
      All those changes were observed in FLP, but not MLP. Our results suggest that 
      NF-kappaB may be involved in progression of the chronic degenerative condition of the 
      mare endometrium, on both canonical and noncanonical pathways. The most important 
      changes in target genes expression were observed only in FLP, which may suggest 
      the hormone-dependent activation of the NF-kappaB-dependent fibrosis pathway.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - Domino, Malgorzata
AU  - Domino M
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Jasinski, Tomasz
AU  - Jasinski T
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Kautz, Ewa
AU  - Kautz E
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Juszczuk-Kubiak, Edyta
AU  - Juszczuk-Kubiak E
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Ferreira-Dias, Graca
AU  - Ferreira-Dias G
AD  - Department of Morphology and Function, CIISA- Centre for Interdisciplinary 
      Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 
      Lisbon, Portugal.
FAU - Zabielski, Romuald
AU  - Zabielski R
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Sady, Maria
AU  - Sady M
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland.
FAU - Gajewski, Zdzislaw
AU  - Gajewski Z
AD  - Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, 
      Veterinary Research Centre and Center for Biomedical Research, Warsaw University 
      of Life Sciences (WULS - SGGW), Warsaw, Poland. Electronic address: 
      zgajewski@supermedia.pl.
LA  - eng
PT  - Journal Article
DEP - 20200129
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Estrous Cycle
MH  - Female
MH  - Fibrosis/metabolism/*veterinary
MH  - Gene Expression Regulation/*physiology
MH  - Horse Diseases/*metabolism
MH  - Horses
MH  - NF-kappa B/genetics/*metabolism
MH  - Uterine Diseases/metabolism/*veterinary
OTO - NOTNLM
OT  - Endometrium
OT  - Endometrosis
OT  - Fibrosis
OT  - HAS
OT  - IL-6
OT  - Mare
OT  - NF-kappaB
COIS- Declaration of competing interest The authors declare that there is no conflict 
      of interests regarding the publication of this paper.
EDAT- 2020/02/20 06:00
MHDA- 2021/01/27 06:00
CRDT- 2020/02/20 06:00
PHST- 2019/09/13 00:00 [received]
PHST- 2019/12/29 00:00 [revised]
PHST- 2020/01/28 00:00 [accepted]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2021/01/27 06:00 [medline]
PHST- 2020/02/20 06:00 [entrez]
AID - S0093-691X(20)30068-6 [pii]
AID - 10.1016/j.theriogenology.2020.01.055 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Apr 15;147:18-24. doi: 10.1016/j.theriogenology.2020.01.055. 
      Epub 2020 Jan 29.