PMID- 32074671
OWN - NLM
STAT- MEDLINE
DCOM- 20200310
LR  - 20200310
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 55
IP  - 2
DP  - 2020 Feb 9
TI  - [Chlorhexidine-grafted coating to improve antibacterial property of the 
      micro/nanoporous titanium surfaces].
PG  - 104-110
LID - 10.3760/cma.j.issn.1002-0098.2020.02.006 [doi]
AB  - Objective: To investigate the antibacterial properties and the 
      osteoblast-compatibility of chlorhexidine (CHX)-modified porous titanium. 
      Methods: Smooth pure titanium specimen with diameter of 10.0 mm and thickness of 
      1.5 mm treated with alkali heat method were set as control group. Those with 
      covalent conjugation of aminosilane were set as silane group, and those with CHX 
      grafted by glutaraldehyde were set as CHX group. Scanning electron microscope 
      (SEM) was used to observe the surface morphology and element compositions were 
      detected by X-ray photoelectron spectroscopy. Hydrophilicity was analyzed by 
      surface water contact angle test (n=6), while surface amino/imine groups 
      quantification were performed through acid orangeⅡ(n=5) and the CHX was 
      quantified by optical densitometric method (n=5). Live/dead bacterial staining, 
      the morphology of adherent bacteria by SEM, plate counting method and inhibition 
      zone method were executed to evaluate the antibacterial property of the samples. 
      Osteoblast compatibility was evaluated by methyl thiazolyl tetrazolium. 
      Cell-bacterial co-culture was conducted to evaluated the cell viability on the 
      samples under the circumstance with bacteria. Results: After CHX grafting, pores 
      on the titanium surface were decreased, while the atom ratio of C, N, Cl 
      increased and the water contact angle decreased to 37.5 degrees +/-4.0 degrees . The density of CHX 
      on the surface was (5.07+/-0.39) mug/cm(2). The results of live/dead bacterial 
      staining and the morphology of adherent bacteria showed that only little dead 
      bacterial (bacterial wall rupture) adherent on the surface of CHX group, which 
      proved that the modified surface could inhibit bacteria adhesion and even 
      destroyed bacteria; the plate counting displayed sporadic colonies and a 
      transparent inhibition zone could be observed, which demonstrated that CHX group 
      could suppress bacteria multiplication from surrounding environment. When 
      incubating for 1 and 3 days, the cell viability of CHX group showed no 
      significant difference from that of control group (P>0.05) ; when incubating for 
      5 days, the value of cell viability of CHX group was 0.547+/-0.087, and this was 
      significantly lower than that of the control group (0.751+/-0.056) (P<0.05), 
      demonstrating a slight inhibition of cell proliferation by CHX. The results of 
      bacteria-cell co-culture for 3 days showed that a mass of bacteria adhered on the 
      surface of the control group while considerable cells adhered on the surface of 
      CHX group and exhibited a good shape. Conclusions: Porous titanium surface 
      grafted by CHX showed an excellent antibacterial properties and allowed cell 
      adhesion in bacterial circumstance, providing immediate implantation options for 
      patients with bad oral health.
FAU - Yang, Y M
AU  - Yang YM
AD  - Teaching and Research Section of Dental Materials, Stomatologic Hospital and 
      College, Anhui Medical University & Key Laboratory of Oral Diseases Research of 
      Anhui Province, Hefei 230032, China.
FAU - Wang, S
AU  - Wang S
FAU - Li, J J
AU  - Li JJ
FAU - Ning, Y J
AU  - Ning YJ
FAU - Li, W
AU  - Li W
FAU - Chen, J L
AU  - Chen JL
LA  - chi
GR  - 31670967/National Natural Science Foundation of China/
GR  - 2016jyxm0508/Anhui Provincial Quality Engineering Teaching Research Project/
GR  - 2017zhyx19/Scientific Research Foundation of the Institute for Translational 
      Medicine of Anhui Province of China/
GR  - gxyq2018007/Anhui Province University Outstanding Youth Talent Support Program/
GR  - 2018B264/Postdoctoral Science Foundation of Anhui Province of China/
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (Anti-Bacterial Agents)
RN  - D1JT611TNE (Titanium)
RN  - R4KO0DY52L (Chlorhexidine)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteria/drug effects
MH  - Bacterial Adhesion/drug effects
MH  - Cells, Cultured
MH  - Chlorhexidine/*pharmacology
MH  - Humans
MH  - *Nanopores
MH  - Osteoblasts/*drug effects
MH  - Surface Properties
MH  - Titanium/*pharmacology
OTO - NOTNLM
OT  - Chlorhexidine
OT  - Microbial sensitivity tests
OT  - Titanium
EDAT- 2020/02/20 06:00
MHDA- 2020/03/11 06:00
CRDT- 2020/02/20 06:00
PHST- 2020/02/20 06:00 [entrez]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2020/03/11 06:00 [medline]
AID - 10.3760/cma.j.issn.1002-0098.2020.02.006 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2020 Feb 9;55(2):104-110. doi: 
      10.3760/cma.j.issn.1002-0098.2020.02.006.