PMID- 32074710
OWN - NLM
STAT- MEDLINE
DCOM- 20200227
LR  - 20200227
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 54
IP  - 2
DP  - 2020 Feb 6
TI  - [Study on fibrosis changes in the lungs of mice caused by repeated inhalation of 
      polyhexamethyleneguaidine disinfectant].
PG  - 198-202
LID - 10.3760/cma.j.issn.0253-9624.2020.02.016 [doi]
AB  - Objective: To explore the lung damage caused by repeated inhalation of 
      polyhexamethyleneguanidine (PHMG) disinfectant aerosol and the corresponding 
      toxicological characteristics. Methods: Thirty four-week-old mice of C57BL/6N 
      strain were randomly divided into three groups, the control group, low-dose 
      group, and high-dose group. Each group had 5 male mice and 5 female mice. Lab 
      II-level purified water was used in the control group. The PHMG disinfectant 
      aerosol was generated by using the ultrasonic atomization of the aqueous solution 
      containing PHMG. The PHMG concentrations in the low-and high-dose groups were 0.1 
      mg/ml (0.01%) and 1 mg/ml (0.1%), respectively. The concentration of PHMG in the 
      post-chemical exposure room was 1.03 mg/m(3) and 9.09 mg/m(3) according to the 
      air sampler analysis. The experimental mice were exposed to the PHMG in dynamic 
      respiratory exposure mode for 4 hours every day in 21 days. After 21-day 
      exposure, bronchia alveolus lung fluids (BALFs) were used to evaluate the 
      inflammatory cells in the lungs, and pathological evaluation, special staining 
      and immunohistochemical methods were further performed to evaluate the key 
      indicators of pulmonary fibrosis. Results: Compared to the control group, the 
      body weight of mice in the high-dose group was significantly decreased (P<0.05), 
      while that of mice in the low-dose group did not significantly differ (P>0.05). 
      The number of inflammatory cells in BALFs of low-dose exposed mice was slightly 
      reduced, and the lung tissue pathology began to show lung damage with early 
      fibrosis symptoms (P<0.05). The pathological examination of mice in the high-dose 
      group showed changes in pulmonary fibrosis. Immunohistochemical staining showed 
      that pulmonary fibrosis marker, alpha-SMA, was significantly increased in low-dose 
      group and high-dose group (P<0.05). Conclusion: The repeated inhalation of PHMG 
      disinfectant could cause lung damage such as pulmonary fibrosis in mice. It could 
      suggest that special warnings should be given to this common disinfectant and 
      respiratory protection measures should be adopted during industrial production 
      and daily use.
FAU - Zhu, X X
AU  - Zhu XX
AD  - School of Public Health, Qingdao University, Qingdao 266021, China.
FAU - Meng, T
AU  - Meng T
AD  - School of Medicine, Datong University, Taiyuan 037009, China.
FAU - Leng, S G
AU  - Leng SG
AD  - School of Public Health, Qingdao University, Qingdao 266021, China.
FAU - Zheng, Y X
AU  - Zheng YX
AD  - School of Public Health, Qingdao University, Qingdao 266021, China.
FAU - Tang, J L
AU  - Tang JL
AD  - School of Public Health, Qingdao University, Qingdao 266021, China.
LA  - chi
GR  - 81872651/National Natural Science Foundation of China/
PT  - Journal Article
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (Disinfectants)
RN  - 0 (Guanidines)
RN  - 31961-54-3 (polyhexamethyleneguanidine)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Disinfectants/administration & dosage/*toxicity
MH  - Female
MH  - Guanidines/administration & dosage/*toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pulmonary Fibrosis/*chemically induced/*pathology
MH  - Random Allocation
OTO - NOTNLM
OT  - Disinfectant
OT  - Lung
OT  - Polyhexamethyleneguanide
OT  - Pulmonary fibrosis
OT  - Toxic actions
EDAT- 2020/02/20 06:00
MHDA- 2020/02/28 06:00
CRDT- 2020/02/20 06:00
PHST- 2020/02/20 06:00 [entrez]
PHST- 2020/02/20 06:00 [pubmed]
PHST- 2020/02/28 06:00 [medline]
AID - 10.3760/cma.j.issn.0253-9624.2020.02.016 [doi]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2020 Feb 6;54(2):198-202. doi: 
      10.3760/cma.j.issn.0253-9624.2020.02.016.