PMID- 32075688
OWN - NLM
STAT- MEDLINE
DCOM- 20200825
LR  - 20210110
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 51
IP  - 1
DP  - 2020 Feb 19
TI  - Effect of polymorphisms in porcine guanylate-binding proteins on host resistance 
      to PRRSV infection in experimentally challenged pigs.
PG  - 14
LID - 10.1186/s13567-020-00745-5 [doi]
LID - 14
AB  - Guanylate-binding proteins (GBP1 and GBP5) are known to be important for host 
      resistance against porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection. In this study, the effects of polymorphisms in GBP1 (GBP1E2 and WUR) 
      and GBP5 on host immune responses against PRRSV were investigated to elucidate 
      the mechanisms governing increased resistance to this disease. Seventy-one pigs 
      [pre-genotyped based on three SNP markers (GBP1E2, WUR, and GBP5)] were assigned 
      to homozygous (n = 36) and heterozygous (n = 35) groups and challenged with the 
      JA142 PRRSV strain. Another group of nineteen pigs was kept separately as a 
      negative control group. Serum and peripheral blood mononuclear cells (PBMCs) were 
      collected at 0, 3, 7, 14, 21 and 28 days post-challenge (dpc). Viremia and weight 
      gain were measured in all pigs at each time point, and a flow cytometry analysis 
      of PBMCs was performed to evaluate T cell activation. In addition, 15 pigs (5 
      pigs per homozygous, heterozygous and negative groups) were sacrificed at 3, 14 
      and 28 dpc, and the local T cell responses were evaluated in the lungs, 
      bronchoalveolar lavage cells (BALc), lymph nodes and tonsils. The heterozygous 
      pigs showed lower viral loads in the serum and lungs and higher weight gains than 
      the homozygous pigs based on the area under the curve calculation. Consistently, 
      compared with the homozygous pigs, the heterozygous pigs exhibited significantly 
      higher levels of IFN-alpha in the serum, proliferation of various T cells (gammadeltaT, Th1, 
      and Th17) in PBMCs and tissues, and cytotoxic T cells in the lungs and BALc. 
      These results indicate that the higher resistance in the pigs heterozygous for 
      the GBP1E2, WUR and GBP5 markers could be mediated by increased antiviral 
      cytokine (IFN-alpha) production and T cell activation.
FAU - Khatun, Amina
AU  - Khatun A
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
AD  - Department of Pathology, Faculty of Animal Science and Veterinary Medicine, 
      Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh.
FAU - Nazki, Salik
AU  - Nazki S
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
FAU - Jeong, Chang-Gi
AU  - Jeong CG
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
FAU - Gu, Suna
AU  - Gu S
AD  - College of Environmental & Biosource Science, Division of Biotechnology, Jeonbuk 
      National University, Iksan, Jeollabuk-do, 54596, South Korea.
FAU - Mattoo, Sameer Ul Salam
AU  - Mattoo SUS
AD  - College of Environmental & Biosource Science, Division of Biotechnology, Jeonbuk 
      National University, Iksan, Jeollabuk-do, 54596, South Korea.
FAU - Lee, Sim-In
AU  - Lee SI
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
FAU - Yang, Myun-Sik
AU  - Yang MS
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
FAU - Lim, Byeonghwi
AU  - Lim B
AD  - College of Agriculture, Life & Environment Sciences, Department of Animal 
      Science, Chungbuk National University, Cheongju, South Korea.
FAU - Kim, Kwan-Suk
AU  - Kim KS
AD  - College of Agriculture, Life & Environment Sciences, Department of Animal 
      Science, Chungbuk National University, Cheongju, South Korea.
FAU - Kim, Bumseok
AU  - Kim B
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea.
FAU - Lee, Kyoung-Tae
AU  - Lee KT
AD  - National Institute of Animal Science, Rural Development Administration, Cheonan, 
      South Korea.
FAU - Park, Choi-Kyu
AU  - Park CK
AD  - College of Veterinary Medicine, Kyoungpook National University, Daegu, South 
      Korea.
FAU - Lee, Sang-Myeong
AU  - Lee SM
AD  - College of Environmental & Biosource Science, Division of Biotechnology, Jeonbuk 
      National University, Iksan, Jeollabuk-do, 54596, South Korea. 
      leesangm@jbnu.ac.kr.
FAU - Kim, Won-Il
AU  - Kim WI
AUID- ORCID: 0000-0002-0465-0794
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, Jeollabuk-do, 
      54596, South Korea. kwi0621@jbnu.ac.kr.
LA  - eng
GR  - 315029-3/The Ministry of Food, Agriculture, Forestry and Fisheries in the 
      Republic of Korea/
GR  - PJ01181601/The Rural Development Administration, the Republic of Korea/
PT  - Journal Article
DEP - 20200219
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - *Disease Resistance
MH  - Female
MH  - GTP-Binding Proteins/*genetics/metabolism
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Swine
PMC - PMC7031929
COIS- The authors declare that they have no competing interests.
EDAT- 2020/02/23 06:00
MHDA- 2020/08/26 06:00
PMCR- 2020/02/19
CRDT- 2020/02/21 06:00
PHST- 2019/09/04 00:00 [received]
PHST- 2020/01/14 00:00 [accepted]
PHST- 2020/02/21 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/08/26 06:00 [medline]
PHST- 2020/02/19 00:00 [pmc-release]
AID - 10.1186/s13567-020-00745-5 [pii]
AID - 745 [pii]
AID - 10.1186/s13567-020-00745-5 [doi]
PST - epublish
SO  - Vet Res. 2020 Feb 19;51(1):14. doi: 10.1186/s13567-020-00745-5.