PMID- 32077869
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20210604
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 7
DP  - 2020 Apr 7
TI  - An electronic enzyme-linked immunosorbent assay platform for protein analysis 
      based on magnetic beads and AlGaN/GaN high electron mobility transistors.
PG  - 2725-2730
LID - 10.1039/c9an01809c [doi]
AB  - AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted 
      attention due to their high sensitivity, stability, and fast response 
      characteristics. Some related studies have been explored but a Debye screening 
      problem exists in physiological solutions hindering the detection of 
      bio-macromolecules. Herein, a novel fast analytical platform for electronic 
      enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT 
      with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the 
      sensing surface to simplify the assay. Combining the advantages of e-ELISA and 
      MBs, the resulting analytical platform presents a sensing capability beyond the 
      Debye-screening limit and a novel ability to be reused. This platform offers a 
      fast response toward prostate specific antigen (PSA) and the lowest concentration 
      of detection is 1 fg mL(-1). Compared with conventional AlGaN/GaN HEMT 
      biosensors, it shows higher sensitivity (3.73 muA dec(-1)) in a linear range (1 fg 
      mL(-1) to 1 pg mL(-1)), which is within the constraints of emergency care 
      applications. The platform's high sensitivity and fast repeatability endow it 
      with great potential for early and rapid diagnosis.
FAU - Wang, Jin
AU  - Wang J
AD  - Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou 
      Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 
      215125, People's Republic of China. jdli2009@sinano.ac.cn.
FAU - Gu, Zhiqi
AU  - Gu Z
FAU - Liu, Xinsheng
AU  - Liu X
FAU - Zhao, Lei
AU  - Zhao L
FAU - Peng, Huoxiang
AU  - Peng H
FAU - Li, Jiadong
AU  - Li J
LA  - eng
PT  - Journal Article
DEP - 20200220
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aluminum Compounds)
RN  - 0 (aluminum gallium nitride)
RN  - 1R9CC3P9VL (gallium nitride)
RN  - CH46OC8YV4 (Gallium)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
EIN - Analyst. 2021 Jun 28;146(13):4400. PMID: 34085671
MH  - Aluminum Compounds/*chemistry
MH  - Biosensing Techniques/*methods
MH  - Electrons
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Gallium/*chemistry
MH  - Humans
MH  - Immunomagnetic Separation
MH  - Limit of Detection
MH  - Male
MH  - Prostate-Specific Antigen/*analysis/isolation & purification
MH  - Reproducibility of Results
MH  - *Transistors, Electronic
EDAT- 2020/02/23 06:00
MHDA- 2020/12/29 06:00
CRDT- 2020/02/21 06:00
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2020/02/21 06:00 [entrez]
AID - 10.1039/c9an01809c [doi]
PST - ppublish
SO  - Analyst. 2020 Apr 7;145(7):2725-2730. doi: 10.1039/c9an01809c. Epub 2020 Feb 20.