PMID- 32081062 OWN - NLM STAT- MEDLINE DCOM- 20201102 LR - 20210327 IS - 1524-4571 (Electronic) IS - 0009-7330 (Print) IS - 0009-7330 (Linking) VI - 126 IP - 7 DP - 2020 Mar 27 TI - Ube2v1 Positively Regulates Protein Aggregation by Modulating Ubiquitin Proteasome System Performance Partially Through K63 Ubiquitination. PG - 907-922 LID - 10.1161/CIRCRESAHA.119.316444 [doi] AB - RATIONALE: Compromised protein quality control can result in proteotoxic intracellular protein aggregates in the heart, leading to cardiac disease and heart failure. Defining the participants and understanding the underlying mechanisms of cardiac protein aggregation is critical for seeking therapeutic targets. We identified Ube2v1 (ubiquitin-conjugating enzyme E2 variant 1) in a genome-wide screen designed to identify novel effectors of the aggregation process. However, its role in the cardiomyocyte is undefined. OBJECTIVE: To assess whether Ube2v1 regulates the protein aggregation caused by cardiomyocyte expression of a mutant alphaB crystallin (CryAB(R120G)) and identify how Ube2v1 exerts its effect. METHODS AND RESULTS: Neonatal rat ventricular cardiomyocytes were infected with adenoviruses expressing either wild-type CryAB (CryAB(WT)) or CryAB(R120G). Subsequently, loss- and gain-of-function experiments were performed. Ube2v1 knockdown decreased aggregate accumulation caused by CryAB(R120G) expression. Overexpressing Ube2v1 promoted aggregate formation in CryAB(WT) and CryAB(R120G)-expressing neonatal rat ventricular cardiomyocytes. Ubiquitin proteasome system performance was analyzed using a ubiquitin proteasome system reporter protein. Ube2v1 knockdown improved ubiquitin proteasome system performance and promoted the degradation of insoluble ubiquitinated proteins in CryAB(R120G) cardiomyocytes but did not alter autophagic flux. Lys (K) 63-linked ubiquitination modulated by Ube2v1 expression enhanced protein aggregation and contributed to Ube2v1's function in regulating protein aggregate formation. Knocking out Ube2v1 exclusively in cardiomyocytes by using AAV9 (adeno-associated virus 9) to deliver multiplexed single guide RNAs against Ube2v1 in cardiac-specific Cas9 mice alleviated CryAB(R120G)-induced protein aggregation, improved cardiac function, and prolonged lifespan in vivo. CONCLUSIONS: Ube2v1 plays an important role in protein aggregate formation, partially by enhancing K63 ubiquitination during a proteotoxic stimulus. Inhibition of Ube2v1 decreases CryAB(R120G)-induced aggregate formation through enhanced ubiquitin proteasome system performance rather than autophagy and may provide a novel therapeutic target to treat cardiac proteinopathies. FAU - Xu, Na AU - Xu N AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Gulick, James AU - Gulick J AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Osinska, Hanna AU - Osinska H AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Yu, Yang AU - Yu Y AD - Division of Developmental Biology (Y.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - McLendon, Patrick M AU - McLendon PM AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Shay-Winkler, Kritton AU - Shay-Winkler K AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Robbins, Jeffrey AU - Robbins J AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. FAU - Yutzey, Katherine E AU - Yutzey KE AD - From the Division of Molecular Cardiovascular Biology (N.X., J.G., H.O., P.M.M., K.S.-W., J.R., K.E.Y.), Department of Pediatrics, Cincinnati Children's Medical Center, OH. LA - eng GR - R01 HL135848/HL/NHLBI NIH HHS/United States GR - P01 HL069779/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20200221 PL - United States TA - Circ Res JT - Circulation research JID - 0047103 RN - 0 (Transcription Factors) RN - 0 (alpha-Crystallin B Chain) RN - EC 2.3.2.23 (UBE2V1 protein, human) RN - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes) RN - EC 3.4.25.1 (Proteasome Endopeptidase Complex) RN - K3Z4F929H6 (Lysine) SB - IM MH - Animals MH - Animals, Newborn MH - Cells, Cultured MH - Female MH - Genome-Wide Association Study/methods MH - Humans MH - Lysine/*metabolism MH - Male MH - Mice, Transgenic MH - Mutation MH - Myocytes, Cardiac/metabolism MH - Proteasome Endopeptidase Complex/*metabolism MH - Protein Aggregation, Pathological/genetics/*metabolism MH - Rats MH - Transcription Factors/genetics/*metabolism MH - Ubiquitin-Conjugating Enzymes/genetics/*metabolism MH - *Ubiquitination MH - alpha-Crystallin B Chain/genetics/metabolism PMC - PMC7135969 MID - NIHMS1564830 OTO - NOTNLM OT - cardiomyocytes OT - heart failure OT - protein aggregates OT - signal transduction EDAT- 2020/02/23 06:00 MHDA- 2020/11/03 06:00 PMCR- 2021/03/27 CRDT- 2020/02/22 06:00 PHST- 2020/02/23 06:00 [pubmed] PHST- 2020/11/03 06:00 [medline] PHST- 2020/02/22 06:00 [entrez] PHST- 2021/03/27 00:00 [pmc-release] AID - 10.1161/CIRCRESAHA.119.316444 [doi] PST - ppublish SO - Circ Res. 2020 Mar 27;126(7):907-922. doi: 10.1161/CIRCRESAHA.119.316444. Epub 2020 Feb 21.