PMID- 32081770
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20210607
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 51
DP  - 2020 Jun
TI  - Selective growth of mosaic cells in chromosomal analysis of chorionic villi by 
      conventional karyotyping.
PG  - 101532
LID - S0890-8508(19)30396-2 [pii]
LID - 10.1016/j.mcp.2020.101532 [doi]
AB  - The major cause of first-trimester pregnancy loss is chromosomal abnormality, 
      which could be detected by many methods. Conventional karyotyping based on 
      chorionic villi (CV) culture is frequently used but may have limitations due to 
      culture failure and selective growth of cells. In this study, we aimed to 
      investigate the degree of mosaicism present in villi by a combination of three 
      different methods, namely conventional karyotyping following culture, multiplex 
      ligation-dependent probe amplification (MLPA) and fluorescence in situ 
      hybridization (FISH), with a view to exploring the incidence of selective growth 
      of mosaic CV cells during the process of culture for conventional karyotyping. CV 
      samples were obtained from 207 patients with early spontaneous miscarriage (ESM). 
      There were 56 (56/207, 27.1%) samples with mosaic chromosome detected by FISH 
      based on four or five types of probes in this study. The incidence of selective 
      growth of mosaic cells during the process of conventional karyotyping was 6.0% 
      (11/183). In addition, we found that mosaic cell lines as low as 2% could grow 
      and completely dominate the outcome of karyotyping results. The selective growth 
      of a particular cell line during culture, whether euploidy or aneuploidy, could 
      supress the diagnosis of mosaicism.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhang, Yingying
AU  - Zhang Y
AD  - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China; 
      Department of Obstetrics and Gynecology, Chinese University of Hong Kong, Shatin, 
      Hong Kong, 999077, PR China.
FAU - Lei, Qiong
AU  - Lei Q
AD  - The Department of Obstetrics, Women and Children's Hospital of Guangdong 
      Province, Guangzhou, 510080, PR China.
FAU - Liu, Juan
AU  - Liu J
AD  - The Department of Gynecology, Traditional Medical Hospital of Guangdong Province, 
      Guangzhou, 510080, PR China.
FAU - Lin, Mingmei
AU  - Lin M
AD  - Department of Obstetrics, Third Affiliated Hospital of Peking University, 
      Beijing, 100191, PR China.
FAU - Luo, Lu
AU  - Luo L
AD  - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China.
FAU - Li, Tinchiu
AU  - Li T
AD  - Department of Obstetrics and Gynecology, Chinese University of Hong Kong, Shatin, 
      Hong Kong, 999077, PR China.
FAU - Wang, Qiong
AU  - Wang Q
AD  - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China. 
      Electronic address: wqiong@mail.sysu.edu.cn.
FAU - Zhou, Canquan
AU  - Zhou C
AD  - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200218
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
SB  - IM
MH  - Abortion, Spontaneous/*genetics
MH  - Adult
MH  - Aneuploidy
MH  - Cells, Cultured
MH  - Chorionic Villi/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Mosaicism
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Risk Factors
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Chorionic villi
OT  - FISH
OT  - Karyotype
OT  - MLPA
OT  - Spontaneous miscarriage
COIS- Declaration of competing interest None.
EDAT- 2020/02/23 06:00
MHDA- 2021/06/08 06:00
CRDT- 2020/02/22 06:00
PHST- 2019/10/18 00:00 [received]
PHST- 2020/02/01 00:00 [revised]
PHST- 2020/02/09 00:00 [accepted]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2020/02/22 06:00 [entrez]
AID - S0890-8508(19)30396-2 [pii]
AID - 10.1016/j.mcp.2020.101532 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2020 Jun;51:101532. doi: 10.1016/j.mcp.2020.101532. Epub 2020 
      Feb 18.