PMID- 32082101
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220412
IS  - 1654-661X (Print)
IS  - 1654-661X (Electronic)
IS  - 1654-661X (Linking)
VI  - 64
DP  - 2020
TI  - Long-term caloric restriction activates the myocardial SIRT1/AMPK/PGC-1alpha pathway 
      in C57BL/6J male mice.
LID - 10.29219/fnr.v64.3668 [doi]
AB  - BACKGROUND: Caloric restriction (CR) can help in improving heart function. There 
      is as yet no consensus on the mechanism of the effect of CR. Silent mating-type 
      information regulation 1 (SIRT1), adenosine monophosphate-activated protein 
      kinase (AMPK), and mTOR are key players in metabolic stress management. We aimed 
      to explore the effect of CR on the myocardial SIRT1/AMPK/mTOR pathway in mice. 
      METHODS: Thirty-six 6-week-old male C57BL/6J mice were randomly divided into 
      three groups: normal control group (NC group, n = 12), high-energy group (HE 
      group, n = 12) and CR group (n = 12) according to different diets. After 11 
      months, western blot was used to examine proteins such as p-AMPK, peroxisome 
      proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha), SIRT1, and 
      p-mTOR, whereas real-time PCR was used to examine the expression of AMPK, PGC-1alpha, 
      and SIRT1 transcripts. RESULTS: Compared to the HE group, the CR group displayed 
      increased expression of myocardial p-AMPK protein, SIRT1 protein and mRNA, and 
      PGC-1a mRNA. However, no difference was observed in the expression of p-mTOR 
      protein and mTOR mRNA in the myocardium among the three groups. CONCLUSIONS: CR 
      improves the SIRT1/AMPK/PGC-1alpha pathway in mice myocardium with no effect on the 
      mTOR pathway.
CI  - (c) 2020 Lina Ma et al.
FAU - Ma, Lina
AU  - Ma L
AD  - Department of Geriatrics, Xuanwu Hospital Capital Medical University, National 
      Clinical Research Center for Geriatric Diseases, Beijing, China.
AD  - Central Laboratory, Xuanwu Hospital Capital Medical University, Key Laboratory 
      for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's 
      Disease, Beijing Institute for Brain Disorders, Beijing, China.
FAU - Wang, Rong
AU  - Wang R
AD  - Central Laboratory, Xuanwu Hospital Capital Medical University, Key Laboratory 
      for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's 
      Disease, Beijing Institute for Brain Disorders, Beijing, China.
FAU - Wang, Hongjuan
AU  - Wang H
AD  - Department of Biochemistry and Molecular Biology, Yanjing Medical College, 
      Capital Medical University, Beijing, China.
FAU - Zhang, Yaxin
AU  - Zhang Y
AD  - Department of Geriatrics, Xuanwu Hospital Capital Medical University, National 
      Clinical Research Center for Geriatric Diseases, Beijing, China.
FAU - Zhao, Zhiwei
AU  - Zhao Z
AD  - Central Laboratory, Xuanwu Hospital Capital Medical University, Key Laboratory 
      for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's 
      Disease, Beijing Institute for Brain Disorders, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200129
PL  - Sweden
TA  - Food Nutr Res
JT  - Food & nutrition research
JID - 101488795
PMC - PMC7007760
OTO - NOTNLM
OT  - AMPK
OT  - SIRT1
OT  - caloric restriction
OT  - mTOR
OT  - signaling pathway
COIS- The authors report no relationships that could be construed as a conflict of 
      interest. This work was supported by the following grants: Beijing Natural 
      Science Foundation (7202059, 7174310), Beijing Municipal Administration of 
      Hospitals Incubating Program (PX2020036), and Nation Natural Science Foundation 
      of China (81600927).
EDAT- 2020/02/23 06:00
MHDA- 2020/02/23 06:01
PMCR- 2020/01/29
CRDT- 2020/02/22 06:00
PHST- 2019/08/11 00:00 [received]
PHST- 2019/12/22 00:00 [revised]
PHST- 2020/01/07 00:00 [accepted]
PHST- 2020/02/22 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/02/23 06:01 [medline]
PHST- 2020/01/29 00:00 [pmc-release]
AID - 3668 [pii]
AID - 10.29219/fnr.v64.3668 [doi]
PST - epublish
SO  - Food Nutr Res. 2020 Jan 29;64. doi: 10.29219/fnr.v64.3668. eCollection 2020.