PMID- 32083129
OWN - NLM
STAT- MEDLINE
DCOM- 20201026
LR  - 20201026
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2020
DP  - 2020
TI  - Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs 
      between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In 
      Vivo.
PG  - 4045204
LID - 10.1155/2020/4045204 [doi]
LID - 4045204
AB  - Alternative splicing (AS) plays a significant role in regulating gene expression 
      at the transcriptional level in eukaryotes. Flexibility and diversity of 
      transcriptome and proteome can be significantly increased through alternative 
      splicing of genes. In the present study, transcriptome data of peripheral immune 
      organs including spleen and inguinal lymph nodes (ILN) were used to identify AS 
      difference between PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible 
      Large White (LW) pigs artificially infected with porcine reproductive and 
      respiratory syndrome virus (PRRSV) in vivo. The results showed that PRRSV 
      infection induced global alternative splicing events (ASEs) with different modes. 
      Among them, 373 genes and 595 genes in the spleen and ILN of TC pigs, while 458 
      genes and 560 genes in the spleen and ILN of LW pigs had significantly 
      differential ASEs. Alternative splicing was subject to tissue-specific and 
      lineage-specific regulation in response to PRRSV infection. Enriched GO terms and 
      pathways showed that genes with differential ASEs played important roles in 
      transcriptional regulation, immune response, metabolism, and apoptosis. 
      Furthermore, a splicing factor associated with apoptosis, SRSF4, was 
      significantly upregulated in LW pigs. Functional analysis on apoptosis associated 
      genes was validated by RT-PCR and DNA sequencing. These findings revealed 
      different response to PRRSV between PRRSV-resistant TC pigs and PRRSV-susceptible 
      LW pigs at the level of alternative splicing, suggesting the potential 
      relationship between AS and disease resistance to PRRSV.
CI  - Copyright (c) 2020 Yu Zhang et al.
FAU - Zhang, Yu
AU  - Zhang Y
AUID- ORCID: 0000-0001-6108-4955
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Xue, Liyao
AU  - Xue L
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Xu, Hang
AU  - Xu H
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Liang, Wan
AU  - Liang W
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Wu, Qingqing
AU  - Wu Q
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Zhang, Qingde
AU  - Zhang Q
AD  - Laboratory Animal Centre, College of Veterinary Medicine, Huazhong Agricultural 
      University, Wuhan 430070, Hubei, China.
FAU - Zhou, Xiang
AU  - Zhou X
AUID- ORCID: 0000-0002-5727-6783
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
FAU - Liu, Bang
AU  - Liu B
AUID- ORCID: 0000-0002-3539-6020
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan 430070, Hubei, China.
LA  - eng
PT  - Journal Article
DEP - 20200130
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Animals
MH  - Apoptosis/genetics
MH  - Disease Resistance/genetics
MH  - Gene Expression Profiling/methods
MH  - Lymph Nodes/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/virology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Serine-Arginine Splicing Factors/genetics
MH  - Spleen/virology
MH  - Swine
MH  - Transcriptome/genetics
MH  - Up-Regulation/genetics
PMC - PMC7011390
COIS- The authors declare no conflicts of interest.
EDAT- 2020/02/23 06:00
MHDA- 2020/10/27 06:00
PMCR- 2020/01/30
CRDT- 2020/02/22 06:00
PHST- 2019/08/27 00:00 [received]
PHST- 2019/11/13 00:00 [accepted]
PHST- 2020/02/22 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/10/27 06:00 [medline]
PHST- 2020/01/30 00:00 [pmc-release]
AID - 10.1155/2020/4045204 [doi]
PST - epublish
SO  - Biomed Res Int. 2020 Jan 30;2020:4045204. doi: 10.1155/2020/4045204. eCollection 
      2020.