PMID- 32083138
OWN - NLM
STAT- MEDLINE
DCOM- 20200624
LR  - 20231103
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2019
DP  - 2019
TI  - HLA Polymorphisms and Haplotype Diversity in Transylvania, Romania.
PG  - 1342762
LID - 10.1155/2019/1342762 [doi]
LID - 1342762
AB  - Transylvania is a historical region in the northwestern part of Romanian with a 
      rather heterogeneous population. Our study is the first to determine human 
      leukocyte antigen (HLA) profiles in a large population sample from this region 
      and to compare them with other European population groups. HLA genes were 
      examined in 2,794 individuals using the Single Specific Primer-Polymerase Chain 
      Reaction (SSP-PCR) and Polymerase Chain Reaction Sequence-Specific 
      Oligonucleotide (PCR-SSO) methods. All samples were tested for the HLA-A locus, 
      2,773 for HLA-B, 1,847 for HLA-C, and 2,719 for HLA-DRB1 loci. HLA gene frequency 
      data from several European population groups (as presented in studies involving 
      more than 1,000 individuals) served as reference in comparison with the local 
      sample. The distribution of HLA genes in the studied population group was 
      heterogeneous, as the Hardy-Weinberg equilibrium was statistically significant (P 
      value < 0.01). The most common genes found in our sample group were A( *)02 
      (0.27%), B( *)35 (0.14%), C( *)07 (0.25%), and DRB1( *)11 (0.19%). The most common 
      haplotype was A( *)01~B( *)08~C( *)07~DRB1( *)03 (1.26% in 1,770 individuals with 
      complete data). This analysis confirmed the known heterogeneity of the 
      Transylvanian population. The study indicates that the European population groups 
      located in close vicinity (those from Serbia, Hungary, Wallachia, and Croatia) 
      are genetically closest to the Transylvanian population.
CI  - Copyright (c) 2019 Mihaela L. Vica et al.
FAU - Vica, Mihaela L
AU  - Vica ML
AD  - Department of Cell and Molecular Biology, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, 6 Pasteur Street, 400 349 Cluj-Napoca, Romania.
FAU - Matei, Horea V
AU  - Matei HV
AUID- ORCID: 0000-0003-2051-3973
AD  - Department of Cell and Molecular Biology, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, 6 Pasteur Street, 400 349 Cluj-Napoca, Romania.
AD  - Legal Medicine Institute Cluj-Napoca, 3-5 Clinicilor Street, 400 006 Cluj-Napoca, 
      Romania.
FAU - Bondor, Cosmina I
AU  - Bondor CI
AUID- ORCID: 0000-0001-6245-4188
AD  - Department of Medical Informatics and Biostatistics, "Iuliu Hatieganu" University 
      of Medicine and Pharmacy, 6 Pasteur Street, 400 349 Cluj-Napoca, Romania.
FAU - Nicula, Gheorghe Z
AU  - Nicula GZ
AD  - Department of Cell and Molecular Biology, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, 6 Pasteur Street, 400 349 Cluj-Napoca, Romania.
FAU - Siserman, Costel V
AU  - Siserman CV
AD  - Legal Medicine Institute Cluj-Napoca, 3-5 Clinicilor Street, 400 006 Cluj-Napoca, 
      Romania.
AD  - Department of Legal Medicine, 'Iuliu Hatieganu' University of Medicine and 
      Pharmacy, 3-5 Clinicilor Street, 400 006 Cluj-Napoca, Romania.
FAU - Loga, Luminita
AU  - Loga L
AD  - Clinical Institute of Urology and Renal Transplant, 2 Clinicilor Street, 400 000 
      Cluj-Napoca, Romania.
FAU - Dican, Lucia
AU  - Dican L
AD  - Clinical Institute of Urology and Renal Transplant, 2 Clinicilor Street, 400 000 
      Cluj-Napoca, Romania.
AD  - Department of Biochemistry, "Iuliu Hatieganu" University of Medicine and 
      Pharmacy, 6 Pasteur Street, 400 349 Cluj-Napoca, Romania.
LA  - eng
PT  - Journal Article
DEP - 20191230
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Gene Frequency
MH  - Genetics, Population
MH  - HLA-A Antigens/blood/genetics
MH  - HLA-B Antigens/blood/genetics
MH  - HLA-C Antigens/blood/genetics
MH  - HLA-DRB1 Chains/blood/genetics
MH  - *Haplotypes
MH  - Histocompatibility Antigens Class I/blood/*genetics
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - Romania/ethnology
MH  - White People/genetics
PMC - PMC7012258
COIS- The authors declare no conflicts of interest in relation to this article.
EDAT- 2020/02/23 06:00
MHDA- 2020/06/25 06:00
PMCR- 2019/12/30
CRDT- 2020/02/22 06:00
PHST- 2019/07/23 00:00 [received]
PHST- 2019/11/06 00:00 [revised]
PHST- 2019/12/10 00:00 [accepted]
PHST- 2020/02/22 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/06/25 06:00 [medline]
PHST- 2019/12/30 00:00 [pmc-release]
AID - 10.1155/2019/1342762 [doi]
PST - epublish
SO  - J Immunol Res. 2019 Dec 30;2019:1342762. doi: 10.1155/2019/1342762. eCollection 
      2019.