PMID- 32083855
OWN - NLM
STAT- MEDLINE
DCOM- 20200326
LR  - 20200326
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 54
IP  - 6
DP  - 2020 Mar 17
TI  - Graphene Oxide-Induced pH Alteration, Iron Overload, and Subsequent Oxidative 
      Damage in Rice (Oryza sativa L.): A New Mechanism of Nanomaterial Phytotoxicity.
PG  - 3181-3190
LID - 10.1021/acs.est.9b05794 [doi]
AB  - The mechanism of graphene-based nanomaterial (GBM)-induced phytotoxicity and its 
      association with the GBM physicochemical properties are not yet fully understood. 
      The present study compared the effects of graphene oxide (GO) and reduced GO 
      (rGO) on rice seedling growth under hydroponic conditions for 3 weeks. GO at 100 
      and 250 mg/L reduced shoot biomass (by 25 and 34%, respectively) and shoot 
      elongation (by 17 and 43%, respectively) and caused oxidative damage, while rGO 
      exhibited no overt effect except for the enhancement of the antioxidant enzyme 
      activities, suggesting that the surface oxygen content is a critical factor 
      affecting the biological impacts of GBMs. GO treatments (100 and 250 mg/L) 
      enhanced the iron (Fe) translocation and caused excessive Fe accumulation in 
      shoots (2.2 and 3.6 times higher than control), which was found to be the main 
      reason for the oxidative damage in shoots. GO-induced acidification of the 
      nutrient solution was the main driver for the Fe overload in plants. In addition 
      to the antioxidant regulators, the plants triggered other pathways to defend 
      against the Fe toxicity via downregulation of the Fe transport associated 
      metabolites (mainly coumarins and flavonoids). Plant root exudates facilitated 
      the reduction of toxic GO to nontoxic rGO, acting as another route for plant 
      adaption to GO-induced phytotoxicity. This study provides new insights into the 
      mechanism of the phytotoxicity of GBMs. It also provides implications for the 
      agricultural application of GBM that the impacts of GBMs on the uptake of 
      multiple nutrients in plants should be assessed simultaneously and reduced forms 
      of GBMs are preferential to avoid toxicity.
FAU - Zhang, Peng
AU  - Zhang P
AUID- ORCID: 0000-0002-2774-5534
AD  - Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute 
      of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
AD  - School of Geography, Earth and Environmental Science, University of Birmingham, 
      Edgbaston, B15 2TT Birmingham, U.K.
FAU - Guo, Zhiling
AU  - Guo Z
AD  - School of Geography, Earth and Environmental Science, University of Birmingham, 
      Edgbaston, B15 2TT Birmingham, U.K.
FAU - Luo, Wenhe
AU  - Luo W
AD  - Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute 
      of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
FAU - Monikh, Fazel Abdolahpur
AU  - Monikh FA
AUID- ORCID: 0000-0001-9500-5303
AD  - Institute of Environmental Sciences (CML), Leiden University, 2300 RA Leiden, 
      Netherlands.
FAU - Xie, Changjian
AU  - Xie C
AD  - Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute 
      of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
FAU - Valsami-Jones, Eugenia
AU  - Valsami-Jones E
AD  - School of Geography, Earth and Environmental Science, University of Birmingham, 
      Edgbaston, B15 2TT Birmingham, U.K.
FAU - Lynch, Iseult
AU  - Lynch I
AUID- ORCID: 0000-0003-4250-4584
AD  - School of Geography, Earth and Environmental Science, University of Birmingham, 
      Edgbaston, B15 2TT Birmingham, U.K.
FAU - Zhang, Zhiyong
AU  - Zhang Z
AUID- ORCID: 0000-0001-9753-0873
AD  - Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, Institute 
      of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200304
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Soil Pollutants)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - *Graphite
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - *Iron Overload
MH  - *Nanostructures
MH  - *Oryza
MH  - Oxidative Stress
MH  - Plant Roots
MH  - Seedlings
MH  - *Soil Pollutants
EDAT- 2020/02/23 06:00
MHDA- 2020/03/27 06:00
CRDT- 2020/02/22 06:00
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/03/27 06:00 [medline]
PHST- 2020/02/22 06:00 [entrez]
AID - 10.1021/acs.est.9b05794 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2020 Mar 17;54(6):3181-3190. doi: 10.1021/acs.est.9b05794. 
      Epub 2020 Mar 4.