PMID- 32084272
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20200708
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 61
IP  - 2
DP  - 2020 Feb 7
TI  - Correlation of Aqueous, Vitreous, and Plasma Cytokine Levels in Patients With 
      Proliferative Diabetic Retinopathy.
PG  - 26
LID - 10.1167/iovs.61.2.26 [doi]
LID - 26
AB  - PURPOSE: To investigate the relationship between proangiogenic and inflammatory 
      cytokines in concurrent vitreous, aqueous, and plasma samples from patients with 
      proliferative diabetic retinopathy (PDR). METHODS: Vitreous, aqueous, and plasma 
      samples were analyzed using multiplex immunoassay for 10 PDR-related cytokines 
      (IL-6, IL-8, TNF-alpha, monocyte chemoattractant protein-1 [MCP-1], macrophage 
      inflammatory protein-1beta [MIP-1beta], VEGF receptor 1 [Flt-1], placental growth 
      factor [PlGF], VEGF-A, VEGF-C, VEGF-D). A total of 17 patients with PDR and 7 
      controls were included. The primary outcome was correlation of cytokines in 
      vitreous, aqueous, and plasma. The secondary outcome was the comparison of 
      cytokine levels in controls and diabetics with and without recent anti-VEGF 
      injection. RESULTS: The following factors were elevated in diabetics compared 
      with controls: vitreous IL-6, IL-8, TNF-alpha, MCP-1, MIP-1beta, PlGF, and VEGF-A; and 
      aqueous IL-6, IL-8, PlGF, and VEGF-C (all P < 0.05). Vitreous and aqueous IL-8, 
      PlGF, and VEGF-A were significantly correlated in patients with PDR (all P < 
      0.05). Plasma cytokines were not correlated with those in vitreous and aqueous 
      (all P > 0.05). Vitreous and aqueous IL-6, IL-8, TNF-alpha, PlGF, and VEGF-A differed 
      among controls and diabetics with and without recent anti-VEGF injection (all P < 
      0.05). In one-to-one comparisons, aqueous VEGF-A levels were lower in diabetic 
      patients who had recent anti-VEGF injection compared with those who did not (P = 
      0.01). CONCLUSIONS: In this proof-of-concept study, IL-8, VEGF-A, and PlGF 
      demonstrated a strong correlation in vitreous and aqueous of patients with PDR. 
      The aqueous may serve as a proxy for vitreous for some cytokines involved in PDR. 
      Recent anti-VEGF injections decreased VEGF-A levels in aqueous, but did not 
      significantly affect other cytokines, suggesting a role for other targeted 
      therapies in PDR management.
FAU - Wu, Frances
AU  - Wu F
AD  - ,.
AD  - ,.
FAU - Phone, Audrey
AU  - Phone A
AD  - ,.
FAU - Lamy, Ricardo
AU  - Lamy R
AD  - ,.
AD  - ,.
FAU - Ma, Dahui
AU  - Ma D
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Laotaweerungsawat, Sawarin
AU  - Laotaweerungsawat S
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Chen, Yi
AU  - Chen Y
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Zhao, Tong
AU  - Zhao T
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Ma, Wei
AU  - Ma W
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Zhang, Fuyan
AU  - Zhang F
AD  - ,.
AD  - ,.
AD  - ,.
FAU - Psaras, Catherine
AU  - Psaras C
AD  - ,.
AD  - ,.
FAU - Stewart, Jay M
AU  - Stewart JM
AD  - ,.
AD  - ,.
LA  - eng
GR  - P30 EY002162/EY/NEI NIH HHS/United States
GR  - R01 EY024004/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Cytokines)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Angiogenesis Inhibitors/therapeutic use
MH  - Aqueous Humor/*metabolism
MH  - Case-Control Studies
MH  - Cytokines/blood/*metabolism
MH  - Diabetic Retinopathy/drug therapy/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Vascular Endothelial Growth Factor A/antagonists & inhibitors/metabolism
MH  - Vitreous Body/*metabolism
PMC - PMC7326572
COIS- Disclosure: F. Wu, None; A. Phone, None; R. Lamy, None; D. Ma, None; S. 
      Laotaweerungsawat, None; Y. Chen, None; T. Zhao, None; W. Ma, None; F. Zhang, 
      None; C. Psaras, None; J.M. Stewart, None
EDAT- 2020/02/23 06:00
MHDA- 2020/04/16 06:00
PMCR- 2020/02/01
CRDT- 2020/02/22 06:00
PHST- 2020/02/22 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - 2761940 [pii]
AID - IOVS-19-28115 [pii]
AID - 10.1167/iovs.61.2.26 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2020 Feb 7;61(2):26. doi: 10.1167/iovs.61.2.26.