PMID- 32084581
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 52
DP  - 2020 Aug
TI  - Fibroblast growth factor-2 ameliorates tumor necrosis factor-alpha-induced 
      osteogenic damage of human bone mesenchymal stem cells by improving oxidative 
      phosphorylation.
PG  - 101538
LID - S0890-8508(20)30004-9 [pii]
LID - 10.1016/j.mcp.2020.101538 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been shown to have an inhibitory effect 
      on the osteogenic differentiation of mesenchymal stem cells. The metabolic switch 
      from glycolysis to oxidative phosphorylation (OXPHOS) is vital for energy supply 
      during osteogenic differentiation. However, the metabolic switch is inhibited 
      under inflammatory stimulation. FGF2 has shown that it can improve osteogenic 
      differentiation and promote autoimmune inflammation. In this study, we 
      investigated whether FGF2 can ameliorate TNF-a-inhibited osteogenic damage by 
      improving OXPHOS. Effects of TNF-alpha or FGF2 on the proliferation and osteogenic 
      differentiation of hBMSCs were evaluated by MTT assay, qRT-PCR, and ALP activity 
      tests. The function of FGF2 on the TNF-a-inhibited metabolic switch was 
      determined by Mito Stress test. The results showed that TNF-alpha was able to inhibit 
      the osteogenic differentiation and OXPHOS of hBMSCs. FGF2 has no obvious function 
      in improving the osteogenic-related genes, but it can ameliorate the impaired 
      osteogenesis and OCR value caused by TNF-alpha. These findings suggest that FGF2 can 
      prevent the impaired osteogenic differentiation and metabolic switch of hBMSCs 
      under inflammatory stimulation, which might enhance the regeneration capacity of 
      hBMSCs.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Hao, Yishan
AU  - Hao Y
AD  - Department of Oral Implantology, Guanghua School of Stomatology, Hospital of 
      Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of 
      Stomatology, Guangzhou, China; Guangdong Provincial Key Laboratory of 
      Stomatology, Sun Yat-sen University, Guangzhou, China.
FAU - Wu, Minting
AU  - Wu M
AD  - Department of Oral Implantology, Guanghua School of Stomatology, Hospital of 
      Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of 
      Stomatology, Guangzhou, China; Guangdong Provincial Key Laboratory of 
      Stomatology, Sun Yat-sen University, Guangzhou, China.
FAU - Wang, Jinming
AU  - Wang J
AD  - Department of Oral Implantology, Guanghua School of Stomatology, Hospital of 
      Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of 
      Stomatology, Guangzhou, China; Guangdong Provincial Key Laboratory of 
      Stomatology, Sun Yat-sen University, Guangzhou, China. Electronic address: 
      wjinm@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200218
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Glycolysis/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/drug effects/*metabolism/*pathology
MH  - Osteogenesis/*drug effects
MH  - Oxidative Phosphorylation/*drug effects
MH  - Tumor Necrosis Factor-alpha/*toxicity
OTO - NOTNLM
OT  - Fibroblast growth factor-2
OT  - Mesenchymal stem cells
OT  - Osteogenic differentiation
OT  - Oxidative phosphorylation
OT  - Tumor necrosis factor-alpha
COIS- Declaration of competing interest The authors have no competing interests to 
      declare.
EDAT- 2020/02/23 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/02/22 06:00
PHST- 2020/01/02 00:00 [received]
PHST- 2020/01/30 00:00 [revised]
PHST- 2020/02/17 00:00 [accepted]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/02/22 06:00 [entrez]
AID - S0890-8508(20)30004-9 [pii]
AID - 10.1016/j.mcp.2020.101538 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2020 Aug;52:101538. doi: 10.1016/j.mcp.2020.101538. Epub 2020 
      Feb 18.