PMID- 32085557
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20240328
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 18
IP  - 2
DP  - 2020 Feb 18
TI  - Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae.
LID - 10.3390/md18020122 [doi]
LID - 122
AB  - Twelve microalgae species isolated in tropical lagoons of New Caledonia were 
      screened as a new source of antioxidants. Microalgae were cultivated at two light 
      intensities to investigate their influence on antioxidant capacity. To assess 
      antioxidant property of microalgae extracts, four assays with different modes of 
      action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis 
      (3-ethylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity 
      (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was 
      coupled to pigment analysis to link antioxidant activity and carotenoid content. 
      The results showed that none of the microalgae studied can scavenge DPPH and ABTS 
      radicals, but Chaetoceros sp., Nephroselmis sp., and Nitzschia A sp. have the 
      capacity to scavenge peroxyl radical (ORAC) and Tetraselmis sp., Nitzschia A sp., 
      and Nephroselmis sp. can inhibit lipid peroxidation (TBARS). Carotenoid 
      composition is typical of the studied microalgae and highlight the 
      siphonaxanthin, detected in Nephroselmis sp., as a pigment of interest. It was 
      found that xanthophylls were the major contributors to the peroxyl radical 
      scavenging capacity measured with ORAC assay, but there was no link between 
      carotenoids and inhibition of lipid peroxidation measured with TBARS assay. In 
      addition, the results showed that light intensity has a strong influence on 
      antioxidant capacity of microalgae: Overall, antioxidant activities measured with 
      ORAC assay are better in high light intensity whereas antioxidant activities 
      measured with TBARS assay are better in low light intensity. It suggests that 
      different antioxidant compounds production is related to light intensity.
FAU - Coulombier, Noemie
AU  - Coulombier N
AD  - ADECAL Technopole, 1 bis rue Berthelot, 98846 Noumea, New Caledonia.
FAU - Nicolau, Elodie
AU  - Nicolau E
AD  - Ifremer, RBE/BRM/PBA, Rue de l'ile d'Yeu, 44311 Nantes, France.
FAU - Le Dean, Loic
AU  - Le Dean L
AD  - Ifremer, UMR 9220 ENTROPIE, RBE/LEAD, 101 Promenade Roger Laroque, 98897 Noumea, 
      New Caledonia.
FAU - Antheaume, Cyril
AU  - Antheaume C
AD  - ISEA, EA7484, Universite de Nouvelle Caledonie, Campus de Nouville, 98851 Noumea, 
      New Caledonia.
FAU - Jauffrais, Thierry
AU  - Jauffrais T
AD  - Ifremer, UMR 9220 ENTROPIE, RBE/LEAD, 101 Promenade Roger Laroque, 98897 Noumea, 
      New Caledonia.
FAU - Lebouvier, Nicolas
AU  - Lebouvier N
AD  - ISEA, EA7484, Universite de Nouvelle Caledonie, Campus de Nouville, 98851 Noumea, 
      New Caledonia.
LA  - eng
GR  - 12/1219969/C./Province Nord, Province Sud, Government of New Caledonia and Comite 
      Interministeriel de l'Outre-Mer/
PT  - Journal Article
DEP - 20200218
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Antioxidants)
RN  - 36-88-4 (Carotenoids)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Carotenoids/analysis/*chemistry/*pharmacology
MH  - Lipid Peroxidation/drug effects
MH  - Microalgae/*chemistry
MH  - New Caledonia
PMC - PMC7073765
OTO - NOTNLM
OT  - bioactive compounds
OT  - carotenoid
OT  - in vitro antioxidant activity
OT  - light intensity
OT  - nephroselmis
OT  - siphonaxanthin
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/23 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/02/01
CRDT- 2020/02/23 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/02/05 00:00 [revised]
PHST- 2020/02/05 00:00 [accepted]
PHST- 2020/02/23 06:00 [entrez]
PHST- 2020/02/23 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - md18020122 [pii]
AID - marinedrugs-18-00122 [pii]
AID - 10.3390/md18020122 [doi]
PST - epublish
SO  - Mar Drugs. 2020 Feb 18;18(2):122. doi: 10.3390/md18020122.