PMID- 32085912 OWN - NLM STAT- MEDLINE DCOM- 20200707 LR - 20231213 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1619 DP - 2020 May 24 TI - Determination of glyphosate and glufosinate in corn using multi-walled carbon nanotubes followed by ultra high performance liquid chromatography coupled with tandem mass spectrometry. PG - 460939 LID - S0021-9673(20)30126-6 [pii] LID - 10.1016/j.chroma.2020.460939 [doi] AB - Glyphosate (Glyp) and glufosinate (Gluf) are widely used herbicides around the world, and their effects on human health and detection of levels have drawn increasing attention. The present study was to establish a method to determine the contents of Glyp and Gluf from corn using multi-walled carbon nanotubes (MWCNTs) followed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). The corn samples were purified by MWCNTs, then the analytes reacted with 9-fluorenylmethylchloroformate (FMOCCl) of acetonitrile solution (20.0 g/L) at 50 degrees C water bath in a borate buffer solution (50.0 g/L, pH=9) to generate FMOC derivative products. After the derivatization, HSS T3 was used as the separation column, with acetonitrile and 0.05% ammonia as the mobile phase, and multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI-) was adopted. The validation parameters showed good verification results, with both of their quantitative limits (LOQ) as 0.005 mg/kg, recoveries between 90.3% and 95.4%, intra-day relative standard deviations (RSDs) in the ranges of 1.24% and 3.35%, and inter-day RSDs between 3.56% and 6.06%. The analytical method, developed in this study, has high accuracy and sensitivity, and is suitable for the simultaneous detection of Glyp and Gluf in corn. CI - Copyright (c) 2020 Elsevier B.V. All rights reserved. FAU - Zhang, Yi AU - Zhang Y AD - Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. FAU - Dang, Yamin AU - Dang Y AD - Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. FAU - Lin, Xiaohui AU - Lin X AD - Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. FAU - An, Kang AU - An K AD - Department of Physical-Chemical Test, School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, 619 Changcheng Road, Daiyue District, Taian 271016, PR China. Electronic address: healthsdu@163.com. FAU - Li, Jianping AU - Li J AD - Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. Electronic address: LJP100@163.com. FAU - Zhang, Mingyue AU - Zhang M AD - Sanitary Inspection Institute, Tianjin Centers for Disease Control and Prevention, 6 Huayue Road, Hedong District, Tianjin 300011, PR China. Electronic address: zhmy201709@163.com. LA - eng PT - Journal Article DEP - 20200208 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 RN - 0 (Acetonitriles) RN - 0 (Aminobutyrates) RN - 0 (Fluorenes) RN - 0 (Herbicides) RN - 0 (Nanotubes, Carbon) RN - 51276-47-2 (phosphinothricin) RN - 9PLB0BTT90 (1-(9-fluorenyl)methyl chloroformate) RN - TE7660XO1C (Glycine) RN - Z072SB282N (acetonitrile) SB - IM MH - Acetonitriles/chemistry MH - Aminobutyrates/*analysis MH - *Chromatography, High Pressure Liquid MH - Fluorenes/chemistry MH - Food Analysis/*methods MH - Glycine/*analogs & derivatives/analysis MH - Herbicides/analysis MH - Nanotubes, Carbon/chemistry MH - Reproducibility of Results MH - *Tandem Mass Spectrometry MH - Zea mays/*chemistry MH - Glyphosate OTO - NOTNLM OT - Corn OT - Glufosinate OT - Glyphosate OT - Multi-walled carbon nanotubes OT - UHPLC-MS/MS COIS- Declaration of Competing Interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted EDAT- 2020/02/23 06:00 MHDA- 2020/07/08 06:00 CRDT- 2020/02/23 06:00 PHST- 2019/10/16 00:00 [received] PHST- 2020/01/06 00:00 [revised] PHST- 2020/01/31 00:00 [accepted] PHST- 2020/02/23 06:00 [pubmed] PHST- 2020/07/08 06:00 [medline] PHST- 2020/02/23 06:00 [entrez] AID - S0021-9673(20)30126-6 [pii] AID - 10.1016/j.chroma.2020.460939 [doi] PST - ppublish SO - J Chromatogr A. 2020 May 24;1619:460939. doi: 10.1016/j.chroma.2020.460939. Epub 2020 Feb 8.