PMID- 32091515
OWN - NLM
STAT- MEDLINE
DCOM- 20201126
LR  - 20201126
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 8
IP  - 8
DP  - 2020 Apr 15
TI  - In vitro cell culture in hollow microfibers with porous structures.
PG  - 2175-2188
LID - 10.1039/c9bm01986c [doi]
AB  - In recent years, there has been a marked increase in tissue engineering research, 
      particularly in the development of electrospun fiber-based substrates as in vitro 
      cell culture platforms. Many scaffolds fabricated via electrospinning have 
      focused on two-dimensional (2D) mats composed of aligned, random or core-shell 
      nanofibers depending on the application and type of extracellular matrix present 
      inside native tissue. In this study, a novel coaxial electrospinning process with 
      a mixture of polylactic-co-glycolic acid (PLGA) and polyethylene oxide (PEO) for 
      sheaths along with polyvinyl alcohol (PVA) for cores was used to produce hollow 
      microfibers. Subsequent removal of PVA and PEO by dissolution resulted in the 
      formation of hollow fibers containing pores inside the shell. The influence of 
      key electrospinning parameters on fiber diameter and pore size was analyzed and 
      optimized via application of design of experiments (DOE). In addition, a 
      regression model was built to show the mathematical relationships and 
      interdependence between process variables and response. Next, the feasibility of 
      this platform for in vitro cell culture was verified by successfully 
      encapsulating and culturing pheochromocytoma 12 (PC12) cells inside the hollow 
      microfibers, where surface perforations could facilitate nutrient and oxygen 
      diffusion and waste removal. After three days of cultivation, it was found that 
      hollow fibers could provide both guidance and support for adherence and 
      proliferation of PC12 cells. This technique based on coaxial cell electrospinning 
      paves the way for development of controllable, tunable and low-cost platforms 
      facilitating guided and constrained culture of cells inside microfibers, thus 
      highlighting its potential as a tool for designing new types of biohybrid 
      materials.
FAU - Ranjan, Vivek Damodar
AU  - Ranjan VD
AD  - NTU Institute for Health Technologies, Interdisciplinary Graduate School, Nanyang 
      Technological University, Singapore 639798.
FAU - Zeng, Peiqin
AU  - Zeng P
AUID- ORCID: 0000-0001-8648-6707
AD  - School of Mechanical & Aerospace Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, Singapore 639798.
FAU - Li, Boyuan
AU  - Li B
AD  - School of Mechanical & Aerospace Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, Singapore 639798.
FAU - Zhang, Yilei
AU  - Zhang Y
AUID- ORCID: 0000-0002-5694-8699
AD  - Department of Mechanical Engineering, University of Canterbury, Christchurch, New 
      Zealand, 8041. yilei.zhang@canterbury.ac.nz.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9002-89-5 (Polyvinyl Alcohol)
SB  - IM
MH  - Animals
MH  - *Cell Culture Techniques
MH  - Cell Proliferation
MH  - Cell Survival
MH  - PC12 Cells
MH  - Polyethylene Glycols/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/chemistry
MH  - Polyvinyl Alcohol/chemistry
MH  - Rats
MH  - Tissue Engineering/methods
MH  - *Tissue Scaffolds
EDAT- 2020/02/25 06:00
MHDA- 2020/11/27 06:00
CRDT- 2020/02/25 06:00
PHST- 2020/02/25 06:00 [pubmed]
PHST- 2020/11/27 06:00 [medline]
PHST- 2020/02/25 06:00 [entrez]
AID - 10.1039/c9bm01986c [doi]
PST - ppublish
SO  - Biomater Sci. 2020 Apr 15;8(8):2175-2188. doi: 10.1039/c9bm01986c.