PMID- 32092857
OWN - NLM
STAT- MEDLINE
DCOM- 20210218
LR  - 20210218
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 2
DP  - 2020 Feb 19
TI  - Novel Lentivirus-Based Method for Rapid Selection of Inhibitory Nanobody against 
      PRRSV.
LID - 10.3390/v12020229 [doi]
LID - 229
AB  - The emergence and re-emergence of porcine reproductive and respiratory syndrome 
      virus (PRRSV) has resulted in huge economic losses for the swine industry. 
      Current vaccines are of limited efficacy against endemic circulating PRRSV 
      variants. New strategies against PRRSV infection are in urgent need. Here, a 
      nanobody library in Marc-145 cells is constructed for antiviral nanobodies. 
      Nanobody encoding sequences from two non-immunized llamas were cloned to generate 
      a pseudotyped lentiviral library. Several candidates were selected from survival 
      cells post-PRRSV inoculation and further characterized. Nb9 was identified with 
      strong antiviral activity. Moreover, Nb9 exerted antiviral activity via its 
      interaction with PRRSV viral proteins, as revealed by immunofluorescence assay 
      and Western blot. Taken together, the novel function-based screen of the 
      lentivirus nanobody library, instead of the conventional affinity-based screen, 
      offers an alternative strategy for antiviral reagents against PRRSV and other 
      pathogens.
FAU - Liu, Ze-Hui
AU  - Liu ZH
AD  - Institute of Preventive Veterinary Medicine, College of Animal Sciences of 
      Zhejiang University, Hangzhou 310058, China.
AD  - Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 
      310058, China.
FAU - Lei, Kai-Xia
AU  - Lei KX
AD  - Institute of Preventive Veterinary Medicine, College of Animal Sciences of 
      Zhejiang University, Hangzhou 310058, China.
AD  - Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 
      310058, China.
FAU - Han, Guang-Wei
AU  - Han GW
AD  - Institute of Preventive Veterinary Medicine, College of Animal Sciences of 
      Zhejiang University, Hangzhou 310058, China.
AD  - Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 
      310058, China.
FAU - Xu, Hui-Ling
AU  - Xu HL
AD  - Institute of Preventive Veterinary Medicine, College of Animal Sciences of 
      Zhejiang University, Hangzhou 310058, China.
AD  - Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 
      310058, China.
FAU - He, Fang
AU  - He F
AD  - Institute of Preventive Veterinary Medicine, College of Animal Sciences of 
      Zhejiang University, Hangzhou 310058, China.
AD  - Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou 
      310058, China.
LA  - eng
PT  - Journal Article
DEP - 20200219
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antiviral Agents)
RN  - 0 (Peptide Library)
RN  - 0 (Single-Domain Antibodies)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Camelids, New World
MH  - Cell Line
MH  - Lentivirus/*genetics
MH  - Peptide Library
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Single-Domain Antibodies/genetics/*pharmacology
MH  - Swine
MH  - Virus Replication/drug effects
PMC - PMC7077216
OTO - NOTNLM
OT  - PRRSV
OT  - antiviral strategy
OT  - lentivirus based nanobody library
OT  - nanobody selection
COIS- The authors declare no conflict of interests.
EDAT- 2020/02/26 06:00
MHDA- 2021/02/20 06:00
PMCR- 2020/02/01
CRDT- 2020/02/26 06:00
PHST- 2019/12/16 00:00 [received]
PHST- 2020/02/11 00:00 [revised]
PHST- 2020/02/17 00:00 [accepted]
PHST- 2020/02/26 06:00 [entrez]
PHST- 2020/02/26 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - v12020229 [pii]
AID - viruses-12-00229 [pii]
AID - 10.3390/v12020229 [doi]
PST - epublish
SO  - Viruses. 2020 Feb 19;12(2):229. doi: 10.3390/v12020229.