PMID- 32093748
OWN - NLM
STAT- MEDLINE
DCOM- 20200825
LR  - 20200825
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 51
IP  - 1
DP  - 2020 Feb 24
TI  - Isolation and characterization of a new population of nasal surface macrophages 
      and their susceptibility to PRRSV-1 subtype 1 (LV) and subtype 3 (Lena).
PG  - 21
LID - 10.1186/s13567-020-00751-7 [doi]
LID - 21
AB  - Sialoadhesin (Sn) and CD163 have been recognized as two important mediators for 
      porcine reproductive and respiratory syndrome virus (PRRSV) in host macrophages. 
      Recently, it has been demonstrated that the highly virulent Lena strain has a 
      wider macrophage tropism than the low virulent LV strain in the nasal mucosa. Not 
      only CD163(+)Sn(+) macrophages are infected by Lena but also CD163(+)Sn(-) 
      macrophages. This suggests that an alternative receptor exists for binding and 
      internalization of PRRSV Lena in the CD163(+)Sn(-) macrophages. Further 
      investigation to find the new entry receptor was hampered by the difficulty of 
      isolating these macrophages from the nasal mucosa. In the present study, a new 
      population of CD163(+)Sn(-) cells has been identified that is specifically 
      localized in the nasal lamina propria and can be isolated by an intranasal 
      digestion approach. Isolated nasal cells were characterized using specific cell 
      markers and their susceptibility to two different PRRSV-1 strains (LV and Lena) 
      was tested. Upon digestion, 3.2% (flow cytometry)-6.4% (confocal microscopy) of 
      the nasal cells were identified as CD163(+) and all (99.7%) of these CD163(+) 
      cells were Sn(-). These CD163(+)Sn(-) cells, designated as "nasal surface 
      macrophages", showed a 4.9 times higher susceptibility to the Lena strain than to 
      the LV strain. Furthermore, the Lena-inoculated cell cultures showed an 
      upregulation of CD163. These results showed that our new cell isolation system is 
      ideal for the further functional and phenotypical analysis of the new population 
      of nasal surface macrophages and further research on the molecular pathogenesis 
      of PRRSV in the nose.
FAU - Oh, Dayoung
AU  - Oh D
AD  - Department of Virology, Immunology, and Parasitology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
FAU - Xie, Jiexiong
AU  - Xie J
AD  - Department of Virology, Immunology, and Parasitology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
FAU - Vanderheijden, Nathalie
AU  - Vanderheijden N
AD  - Department of Virology, Immunology, and Parasitology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
AUID- ORCID: 0000-0001-5470-0713
AD  - Department of Virology, Immunology, and Parasitology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium. 
      Hans.Nauwynck@UGent.be.
LA  - eng
PT  - Journal Article
DEP - 20200224
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*immunology
MH  - Antigens, Differentiation, Myelomonocytic/*immunology
MH  - Cell Culture Techniques
MH  - Macrophages/*immunology
MH  - Nasal Mucosa/immunology/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Receptors, Cell Surface/*immunology
MH  - Sialic Acid Binding Ig-like Lectin 1/immunology
MH  - Swine
PMC - PMC7038536
COIS- The authors declare that they have no competing interests.
EDAT- 2020/02/26 06:00
MHDA- 2020/08/26 06:00
PMCR- 2020/02/24
CRDT- 2020/02/26 06:00
PHST- 2019/11/19 00:00 [received]
PHST- 2020/01/27 00:00 [accepted]
PHST- 2020/02/26 06:00 [entrez]
PHST- 2020/02/26 06:00 [pubmed]
PHST- 2020/08/26 06:00 [medline]
PHST- 2020/02/24 00:00 [pmc-release]
AID - 10.1186/s13567-020-00751-7 [pii]
AID - 751 [pii]
AID - 10.1186/s13567-020-00751-7 [doi]
PST - epublish
SO  - Vet Res. 2020 Feb 24;51(1):21. doi: 10.1186/s13567-020-00751-7.