PMID- 32098268
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20201123
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 20
IP  - 4
DP  - 2020 Feb 21
TI  - 3D-Printed Modular Microfluidic Device Enabling Preconcentrating Bacteria and 
      Purifying Bacterial DNA in Blood for Improving the Sensitivity of Molecular 
      Diagnostics.
LID - 10.3390/s20041202 [doi]
LID - 1202
AB  - Molecular diagnostics for sepsis is still a challenge due to the presence of 
      compounds that interfere with gene amplification and bacteria at concentrations 
      lower than the limit of detection (LOD). Here, we report on the development of a 
      3D printed modular microfluidic device (3DpmmuFD) that preconcentrates bacteria of 
      interest in whole blood and purifies their genomic DNA (gDNA). It is composed of 
      a W-shaped microchannel and a conical microchamber. Bacteria of interest are 
      magnetically captured from blood in the device with antibody conjugated magnetic 
      nanoparticles (Ab-MNPs) at 5 mL/min in the W-shaped microchannel, while purified 
      gDNA of the preconcentrated bacteria is obtained with magnetic silica beads 
      (MSBs) at 2 mL/min in the conical microchamber. The conical microchamber was 
      designed to be connected to the microchannel after the capturing process using a 
      3D-printed rotary valve to minimize the exposure of the MSBs to interfering 
      compounds in blood. The pretreatment process of spiked blood (2.5 mL) can be 
      effectively completed within about 50 min. With the 3DpmmuFD, the LOD for the 
      target microorganism Escherichia coli O157:H7 measured by both polymerase chain 
      reaction (PCR) with electrophoresis and quantitative PCR was 10 colony forming 
      unit (CFU) per mL of whole blood. The results suggest that our method lowers the 
      LOD of molecular diagnostics for pathogens in blood by providing bacterial gDNA 
      at high purity and concentration.
FAU - Abafogi, Abdurhaman Teyib
AU  - Abafogi AT
AUID- ORCID: 0000-0001-5679-1401
AD  - School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
FAU - Kim, Jaewon
AU  - Kim J
AD  - School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
FAU - Lee, Jinyeop
AU  - Lee J
AD  - School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
FAU - Mohammed, Merem Omer
AU  - Mohammed MO
AUID- ORCID: 0000-0003-0456-8923
AD  - Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, 
      Korea.
FAU - van Noort, Danny
AU  - van Noort D
AUID- ORCID: 0000-0002-0818-0443
AD  - Division of Biotechnology, IFM, Linkoping University, 58183 Linkoping, Sweden.
AD  - Chair of Micro Process Engineering and Technology (COMPETE), University of 
      Ljubljana, 1000 Ljubljana, Slovenia.
AD  - Centro de Investigacion en Bioingenieria -BIO, Universidad de Ingenieria y 
      Tecnologia-UTEC, Barranco 15036, Peru.
FAU - Park, Sungsu
AU  - Park S
AUID- ORCID: 0000-0003-3062-1302
AD  - School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea.
AD  - Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, 
      Korea.
AD  - Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 
      Suwon 16419, Korea.
LA  - eng
GR  - H-guard 2018M3A6B2057299/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20200221
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacteria/genetics/*metabolism
MH  - DNA, Bacterial/*metabolism
MH  - Escherichia coli/genetics/metabolism
MH  - *Lab-On-A-Chip Devices
MH  - Pathology, Molecular/*methods
MH  - Polymerase Chain Reaction
PMC - PMC7070462
OTO - NOTNLM
OT  - DNA purification
OT  - bacterial preconcentration
OT  - microfluidic device
OT  - molecular diagnostics
OT  - pathogen
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/27 06:00
MHDA- 2020/11/24 06:00
PMCR- 2020/02/01
CRDT- 2020/02/27 06:00
PHST- 2020/01/17 00:00 [received]
PHST- 2020/02/19 00:00 [revised]
PHST- 2020/02/20 00:00 [accepted]
PHST- 2020/02/27 06:00 [entrez]
PHST- 2020/02/27 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - s20041202 [pii]
AID - sensors-20-01202 [pii]
AID - 10.3390/s20041202 [doi]
PST - epublish
SO  - Sensors (Basel). 2020 Feb 21;20(4):1202. doi: 10.3390/s20041202.