PMID- 32100268
OWN - NLM
STAT- MEDLINE
DCOM- 20210802
LR  - 20210802
IS  - 2190-3948 (Electronic)
IS  - 2190-393X (Linking)
VI  - 10
IP  - 4
DP  - 2020 Aug
TI  - In vitro and in vivo evaluation of gentamicin sulphate-loaded PLGA 
      nanoparticle-based film for the treatment of surgical site infection.
PG  - 1032-1043
LID - 10.1007/s13346-020-00730-7 [doi]
AB  - The study focuses on the in vitro and in vivo evaluation of the developed 
      gentamicin sulphate (GS)-loaded poly lactic-co-glycolic acid (PLGA) nanoparticle 
      (PNP)-based pullulan film (PNP-F). Sterilization being an essential pre-requisite 
      for the dosage form was carried out using ethylene oxide. Post-sterilization, 
      PNP-F was evaluated for mechanical properties, percentage drug loading, 
      antimicrobial effectiveness study, test for sterility and in vitro dissolution 
      study using Strat-M(R) membrane. In vitro dissolution study revealed that GS 
      gradually released from PNP-F and the highest cumulative percentage drug release 
      was found to be 86.76 +/- 0.03% at 192 h. Wound healing assay was performed to 
      study the effect of PNP-F over migratory potential of dermal fibroblast cells 
      (NIH-3T3) in the presence of micro-organisms, Pseudomonas aeruginosa (PA) and 
      Staphylococcus aureus (SA). PNP-F inhibited the growth of PA and SA, allowing the 
      growth of fibroblast cells indicating its suitability for application. In vivo 
      study of surgical site was performed by superficial incision model in Wistar 
      rats. Measurement of in vivo incision healing confirmed faster wound healing in 
      the incision which received PNP-F compared to marketed cream containing GS. 
      Graphical abstract.
FAU - Dhal, Chetan
AU  - Dhal C
AUID- ORCID: 0000-0002-0596-9481
AD  - Department of Pharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, 
      Nirma University, Ahmedabad, 382481, India. dhalchetan@yahoo.co.in.
FAU - Mishra, Renuka
AU  - Mishra R
AD  - Department of Pharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, 
      Nirma University, Ahmedabad, 382481, India.
LA  - eng
GR  - 09/1048(008)/2018 EMR-I/Council of Scientific and Industrial Research, 
      India/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Drug Deliv Transl Res
JT  - Drug delivery and translational research
JID - 101540061
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Gentamicins)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*administration & dosage/chemistry
MH  - Cell Movement/drug effects
MH  - Drug Liberation
MH  - Gentamicins/*administration & dosage/chemistry
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Nanoparticles/*administration & dosage/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/*administration & dosage/chemistry
MH  - Pseudomonas Infections/drug therapy
MH  - Pseudomonas aeruginosa
MH  - Rats, Wistar
MH  - Staphylococcal Infections/drug therapy
MH  - Staphylococcus aureus
MH  - Surgical Wound Infection/*drug therapy
OTO - NOTNLM
OT  - Film
OT  - Gentamicin sulphate
OT  - In vivo study
OT  - Nanoparticles
OT  - Surgical site infection
OT  - Wound healing assay
EDAT- 2020/02/27 06:00
MHDA- 2021/08/03 06:00
CRDT- 2020/02/27 06:00
PHST- 2020/02/27 06:00 [pubmed]
PHST- 2021/08/03 06:00 [medline]
PHST- 2020/02/27 06:00 [entrez]
AID - 10.1007/s13346-020-00730-7 [pii]
AID - 10.1007/s13346-020-00730-7 [doi]
PST - ppublish
SO  - Drug Deliv Transl Res. 2020 Aug;10(4):1032-1043. doi: 10.1007/s13346-020-00730-7.