PMID- 32100923
OWN - NLM
STAT- MEDLINE
DCOM- 20210806
LR  - 20210806
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 34
IP  - 7
DP  - 2020 Jul
TI  - Study on the correlation between hTREC and HPV load and cervical CINI/II/III 
      lesions and cervical cancer.
PG  - e23257
LID - 10.1002/jcla.23257 [doi]
LID - e23257
AB  - OBJECTIVE: To investigate the correlation between hTREC and human papillomavirus 
      (HPV) load and cervical intraepithelial neoplasia (CIN) grade II/III lesions and 
      cervical cancer. METHODS: A total of 135 patients with cervical lesions of 
      different degrees admitted to our hospital from January 2016 to February 2017 
      were selected, including CIN I/III 65 cases, grade III 39 cases, and cervical 
      cancer 31 cases. The expression of hTERC gene was detected by fluorescence in 
      situ hybridization (FISH) in three groups, and the HPV load was detected by 
      second-generation hybridization capture (HC II) method, and its relationship with 
      cervical lesion grade was analyzed. Department. RESULTS: The positive expression 
      rate of hTERC gene amplification was cervical cancer > CIN I/II lesion > CIN III 
      lesion; the positive expression rate of HPV was cervical cancer > CIN I/II 
      lesion > CIN III lesion. After treatment, the positive rate of hTERC gene 
      amplification and HPV expression decreased significantly within 1 year (P < .05). 
      Spearman's analysis showed that the degree of cervical lesion was positively 
      correlated with hTREC and HPV load (P < .05). CONCLUSION: hTREC and HPV are 
      closely related to the occurrence and development of cervical precancerous 
      lesions and cervical cancer. The abnormal amplification of hTERC gene increases 
      with the grade of cervical lesions. Both of them can be used as auxiliary 
      indicators for early screening, treatment, and prognosis of cervical cancer.
CI  - (c) 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals, Inc.
FAU - He, Haizhen
AU  - He H
AD  - Wenzhou People's Hospital Gynecotokology, Wenzhou, China.
FAU - Pan, Qionghui
AU  - Pan Q
AD  - Wenzhou People's Hospital Gynecotokology, Wenzhou, China.
FAU - Pan, Jiajia
AU  - Pan J
AD  - Wenzhou People's Hospital Gynecotokology, Wenzhou, China.
FAU - Chen, Yumei
AU  - Chen Y
AD  - Wenzhou People's Hospital Gynecotokology, Wenzhou, China.
FAU - Cao, Liqin
AU  - Cao L
AUID- ORCID: 0000-0002-0612-0789
AD  - Shanghai TCM-Integarted Hospitalgynecotokology, Shanghai, China.
LA  - eng
GR  - Y20170459;Y20170614/Correlation between abnormal amplification of hTREC gene and 
      expression of P16INK4A and Ki67 in cervical lesions and its significance in the 
      management of CINI shunt;Fund project of Wenzhou science and Technology Bureau/
PT  - Journal Article
DEP - 20200226
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Papillomavirus Infections/*pathology
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/genetics/*pathology/*virology
MH  - *Viral Load
PMC - PMC7370748
OTO - NOTNLM
OT  - cervical cancer
OT  - cervical precancerous lesions
OT  - hTREC
OT  - human papillomavirus
EDAT- 2020/02/27 06:00
MHDA- 2021/08/07 06:00
PMCR- 2020/02/26
CRDT- 2020/02/27 06:00
PHST- 2019/12/10 00:00 [received]
PHST- 2020/01/30 00:00 [revised]
PHST- 2020/02/02 00:00 [accepted]
PHST- 2020/02/27 06:00 [pubmed]
PHST- 2021/08/07 06:00 [medline]
PHST- 2020/02/27 06:00 [entrez]
PHST- 2020/02/26 00:00 [pmc-release]
AID - JCLA23257 [pii]
AID - 10.1002/jcla.23257 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2020 Jul;34(7):e23257. doi: 10.1002/jcla.23257. Epub 2020 Feb 
      26.