PMID- 32101736
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20240328
IS  - 2211-1247 (Electronic)
VI  - 30
IP  - 8
DP  - 2020 Feb 25
TI  - High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional 
      Microscopy Using Smart Line Scanning.
PG  - 2567-2580.e6
LID - S2211-1247(20)30149-2 [pii]
LID - 10.1016/j.celrep.2020.01.105 [doi]
AB  - Two-photon functional imaging using genetically encoded calcium indicators 
      (GECIs) is one prominent tool to map neural activity. Under optimized 
      experimental conditions, GECIs detect single action potentials in individual 
      cells with high accuracy. However, using current approaches, these optimized 
      conditions are never met when imaging large ensembles of neurons. Here, we 
      developed a method that substantially increases the signal-to-noise ratio (SNR) 
      of population imaging of GECIs by using galvanometric mirrors and fast smart line 
      scan (SLS) trajectories. We validated our approach in anesthetized and awake mice 
      on deep and dense GCaMP6 staining in the mouse barrel cortex during spontaneous 
      and sensory-evoked activity. Compared to raster population imaging, SLS led to 
      increased SNR, higher probability of detecting calcium events, and more precise 
      identification of functional neuronal ensembles. SLS provides a cheap and easily 
      implementable tool for high-accuracy population imaging of neural GCaMP6 signals 
      by using galvanometric-based two-photon microscopes.
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Brondi, Marco
AU  - Brondi M
AD  - Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 
      Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova 
      and Rovereto, Italy.
FAU - Moroni, Monica
AU  - Moroni M
AD  - Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, 
      Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive 
      Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, Italy; Center for Mind 
      and Brain Sciences (CIMeC), University of Trento, Trento, Italy.
FAU - Vecchia, Dania
AU  - Vecchia D
AD  - Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 
      Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova 
      and Rovereto, Italy.
FAU - Molano-Mazon, Manuel
AU  - Molano-Mazon M
AD  - Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, 
      Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive 
      Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, Italy.
FAU - Panzeri, Stefano
AU  - Panzeri S
AD  - Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova and Rovereto, 
      Italy; Neural Computation Laboratory, Center for Neuroscience and Cognitive 
      Systems @UniTn, Istituto Italiano di Tecnologia, Rovereto, Italy.
FAU - Fellin, Tommaso
AU  - Fellin T
AD  - Optical Approaches to Brain Function Laboratory, Istituto Italiano di Tecnologia, 
      Genova, Italy; Neural Coding Laboratory, Istituto Italiano di Tecnologia, Genova 
      and Rovereto, Italy. Electronic address: tommaso.fellin@iit.it.
LA  - eng
GR  - U01 NS090576/NS/NINDS NIH HHS/United States
GR  - U19 NS107464/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Artifacts
MH  - Calcium/metabolism
MH  - *Image Processing, Computer-Assisted
MH  - Mice
MH  - *Microscopy, Fluorescence, Multiphoton
MH  - Motion
MH  - Neurons/*physiology
MH  - Neuropil Threads/physiology
MH  - Wakefulness
PMC - PMC7043026
OTO - NOTNLM
OT  - GCaMP6
OT  - barrel cortex
OT  - neuronal ensembles
OT  - spatiotemporal neural responses
OT  - two-photon imaging
COIS- Declaration of Interests The authors declare no competing interests.
EDAT- 2020/02/27 06:00
MHDA- 2021/03/18 06:00
PMCR- 2020/02/25
CRDT- 2020/02/27 06:00
PHST- 2019/06/28 00:00 [received]
PHST- 2020/01/10 00:00 [revised]
PHST- 2020/01/29 00:00 [accepted]
PHST- 2020/02/27 06:00 [entrez]
PHST- 2020/02/27 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
PHST- 2020/02/25 00:00 [pmc-release]
AID - S2211-1247(20)30149-2 [pii]
AID - 10.1016/j.celrep.2020.01.105 [doi]
PST - ppublish
SO  - Cell Rep. 2020 Feb 25;30(8):2567-2580.e6. doi: 10.1016/j.celrep.2020.01.105.