PMID- 32102514
OWN - NLM
STAT- MEDLINE
DCOM- 20201124
LR  - 20201124
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Print)
IS  - 1513-7368 (Linking)
VI  - 21
IP  - 2
DP  - 2020 Feb 1
TI  - Cyclin D1 Gene Numerical Imbalances in Laryngeal Squamous Cell Carcinoma: A 
      Tissue Microarray Grid Based Analysis.
PG  - 379-384
LID - 88935 [pii]
LID - 10.31557/APJCP.2020.21.2.379 [doi]
AB  - BACKGROUND: Deregulation of critical proteins involved in cell cycle stability, 
      such as cyclins, is a frequent genetic event in the development and progression 
      of solid malignancies. Concerning laryngeal squamous cell carcinoma (LSCC), 
      cyclin D1 oncogenic transformation leads to an aberrant protein expression and 
      seems to affect the biological behaviour of the neoplasm. The aim of this study 
      was to determine the correlation of cyclin D1 numerical imbalances with the 
      corresponding protein expression levels in LSCCs. MATERIAL AND METHOD: Using 
      tissue microarray (TMA) technology, fifty (n=50) histologically confirmed primary 
      LSSCs were cored at a diameter of 1.5 mm. Immunohistochemistry (IHC) and 
      chromogenic in situ hybridization (CISH) analyses were applied. Concerning the 
      screening process in CISH slides, a novel real-time reference and calibration 
      grid platform was implemented. RESULTS: Protein overexpression was observed in 
      22/50 (44%) cases; whereas, gene amplification was seen in 13/50 (26%) cases 
      (p=0.02). Combined protein/ gene deregulation was associated with the stage of 
      malignancy (p= 0.0014, p=0.001), whereas overall protein expression was strongly 
      correlated with the grade of tumour (p= 0.001). CONCLUSION: Cyclin D1 gene 
      amplification led to aberrant protein expression in LSCCs and it was also 
      correlated with an aggressive biological behaviour. To best of our knowledge, 
      this study was the first described grid based CISH analysis under conventional 
      bright field microscopy for detecting gene numerical imbalances while providing a 
      novel and accurate description for screening-mapping process in the entire slide 
      area.<br />.
FAU - Kyrodimos, Efthymios
AU  - Kyrodimos E
AD  - 1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, 
      Athens, Greece.
FAU - Papanikolaou, Vasileios
AU  - Papanikolaou V
AD  - 1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, 
      Athens, Greece.
FAU - Tsiambas, Evangelos
AU  - Tsiambas E
AD  - Department of Pathology-Cytology, 401 GAH, Athens, Greece.
AD  - Department of Pathology, 417 VA Hospital (NIMTS), Athens, Greece.
FAU - Kikidis, Dimitrios
AU  - Kikidis D
AD  - 1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, 
      Athens, Greece.
FAU - Peschos, Dimitrios
AU  - Peschos D
AD  - Department of Physiology, Medical School, University of Ioannina, Greece.
FAU - Ragos, Vasileios
AU  - Ragos V
AD  - Department of Maxillofacial, Medical School, University of Ioannina, Greece.
FAU - Mastronikolis, Nicholas
AU  - Mastronikolis N
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Medical School, 
      University of Patras, Greece.
FAU - Riziotis, Christos
AU  - Riziotis C
AD  - Theoretical and Physical Chemistry Institute, Photonics for Nanoapplications 
      Laboratory, National Hellenic Research Foundation, Athens, Greece.
FAU - Chrysovergis, Aristeidis
AU  - Chrysovergis A
AD  - 1st ENT Dept, Hippocration Hospital, Medical School, University of Athens, 
      Athens, Greece.
LA  - eng
PT  - Journal Article
DEP - 20200201
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*genetics/metabolism
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology/surgery
MH  - Cyclin D1/*genetics/metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - *Gene Amplification
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Tissue Array Analysis
PMC - PMC7332136
OTO - NOTNLM
OT  - Carcinoma
OT  - Gene
OT  - Larynx
OT  - cyclin
OT  - grid
EDAT- 2020/02/28 06:00
MHDA- 2020/11/25 06:00
PMCR- 2020/03/01
CRDT- 2020/02/28 06:00
PHST- 2019/06/09 00:00 [received]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2020/11/25 06:00 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - 88935 [pii]
AID - 10.31557/APJCP.2020.21.2.379 [doi]
PST - epublish
SO  - Asian Pac J Cancer Prev. 2020 Feb 1;21(2):379-384. doi: 
      10.31557/APJCP.2020.21.2.379.