PMID- 32102842
OWN - NLM
STAT- MEDLINE
DCOM- 20210128
LR  - 20210128
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 32
IP  - 5
DP  - 2020 May
TI  - Photosynthetic Phosphoribulokinase Structures: Enzymatic Mechanisms and the Redox 
      Regulation of the Calvin-Benson-Bassham Cycle.
PG  - 1556-1573
LID - 10.1105/tpc.19.00642 [doi]
AB  - The Calvin-Benson-Bassham (CBB) cycle is responsible for CO(2) assimilation and 
      carbohydrate production in oxyphototrophs. Phosphoribulokinase (PRK) is an 
      essential enzyme of the CBB cycle in photosynthesis, catalyzing ATP-dependent 
      conversion of ribulose-5-phosphate (Ru5P) to ribulose-1,5-bisphosphate. The 
      oxyphototrophic PRK is redox-regulated and can be further regulated by reversible 
      association with both glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 
      oxidized chloroplast protein CP12. The resulting GAPDH/CP12/PRK complex is 
      central in the regulation of the CBB cycle; however, the PRK-CP12 interface in 
      the recently reported cyanobacterial GAPDH/CP12/PRK structure was not well 
      resolved, and the detailed binding mode of PRK with ATP and Ru5P remains 
      undetermined, as only apo-form structures of PRK are currently available. Here, 
      we report the crystal structures of cyanobacterial (Synechococcus elongatus) PRK 
      in complex with ADP and glucose-6-phosphate and of the Arabidopsis (Arabidopsis 
      thaliana) GAPDH/CP12/PRK complex, providing detailed information regarding the 
      active site of PRK and the key elements essential for PRK-CP12 interaction. Our 
      structural and biochemical results together reveal that the ATP binding site is 
      disrupted in the oxidized PRK, whereas the Ru5P binding site is occupied by 
      oxidized CP12 in the GAPDH/CP12/PRK complex. This structure-function study 
      greatly advances the understanding of the reaction mechanism of PRK and the 
      subtle regulations of redox signaling for the CBB cycle.
CI  - (c) 2020 American Society of Plant Biologists. All rights reserved.
FAU - Yu, Ailing
AU  - Yu A
AUID- ORCID: 0000-0001-8474-4012
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
AD  - University of the Chinese Academy of Sciences, Beijing 100049, People's Republic 
      of China.
FAU - Xie, Yuan
AU  - Xie Y
AUID- ORCID: 0000-0001-6982-8525
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
FAU - Pan, Xiaowei
AU  - Pan X
AUID- ORCID: 0000-0002-9672-4649
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
FAU - Zhang, Hongmei
AU  - Zhang H
AUID- ORCID: 0000-0002-4617-9224
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
FAU - Cao, Peng
AU  - Cao P
AUID- ORCID: 0000-0002-4616-5914
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
FAU - Su, Xiaodong
AU  - Su X
AUID- ORCID: 0000-0001-6650-2327
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
FAU - Chang, Wenrui
AU  - Chang W
AUID- ORCID: 0000-0002-5080-6446
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China.
AD  - University of the Chinese Academy of Sciences, Beijing 100049, People's Republic 
      of China.
FAU - Li, Mei
AU  - Li M
AUID- ORCID: 0000-0002-1742-2360
AD  - National Laboratory of Biomacromolecules, CAS Center for Excellence in 
      Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 
      100101, People's Republic of China meili@ibp.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200225
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Ligands)
RN  - 0 (Ribulosephosphates)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.19 (phosphoribulokinase)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Arabidopsis/*enzymology
MH  - Arabidopsis Proteins/metabolism
MH  - Biocatalysis
MH  - Catalytic Domain
MH  - Ligands
MH  - Models, Molecular
MH  - Oxidation-Reduction
MH  - Phosphotransferases (Alcohol Group Acceptor)/*chemistry/*metabolism
MH  - *Photosynthesis
MH  - Protein Binding
MH  - Protein Structure, Secondary
MH  - Ribulosephosphates/metabolism
MH  - Structural Homology, Protein
MH  - Synechococcus/*enzymology
PMC - PMC7203937
EDAT- 2020/02/28 06:00
MHDA- 2021/01/29 06:00
PMCR- 2020/02/25
CRDT- 2020/02/28 06:00
PHST- 2019/08/19 00:00 [received]
PHST- 2020/01/13 00:00 [revised]
PHST- 2020/02/24 00:00 [accepted]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2021/01/29 06:00 [medline]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/25 00:00 [pmc-release]
AID - tpc.19.00642 [pii]
AID - 201900642R2 [pii]
AID - 10.1105/tpc.19.00642 [doi]
PST - ppublish
SO  - Plant Cell. 2020 May;32(5):1556-1573. doi: 10.1105/tpc.19.00642. Epub 2020 Feb 
      25.