PMID- 32103369
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20210114
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Linking)
VI  - 48
IP  - 6
DP  - 2020 Jun
TI  - Cryo-imaging of Stem Cell Biodistribution in Mouse Model of 
      Graft-Versus-Host-Disease.
PG  - 1702-1711
LID - 10.1007/s10439-020-02487-z [doi]
AB  - We demonstrated the use of multispectral cryo-imaging and software to analyze 
      human mesenchymal stromal cells (hMSCs) biodistribution in mouse models of 
      graft-versus-host-disease (GVHD) following allogeneic bone marrow transplantation 
      (BMT). We injected quantum dot labeled MSCs via tail vein to mice receiving BMT 
      and analyzed hMSC biodistribution in major organs (e.g. lung, liver, spleen, 
      kidneys and bone marrow). We compared the biodistribution of hMSCs in mice 
      following allogeneic BMT recipients (with GVHD) to the biodistribution following 
      syngeneic BMT (without GVHD). Cryo-imaging system revealed cellular 
      biodistribution and redistribution patterns in the animal model. We initially 
      found clusters of cells in the lung that eventually dissociated to single cells 
      and redistributed to other organs within 72 h. The in vivo half-life of the 
      exogenous MSCs was about 21 h. We found that the biodistribution of stromal cells 
      was not related to blood flow, rather cells preferentially homed to specific 
      organs. In conclusion, cryo-imaging was suitable for analyzing the cellular 
      biodistribution. It could provide capabilities of visualizing cells anywhere in 
      the mouse model with single cell sensitivity. By characterizing the 
      biodistribution and anatomical specificity of a therapeutic cellular product, we 
      believe that cryo-imaging can play an important role in the advancement of stem 
      and stromal cell therapies and regenerative medicine.
FAU - Wuttisarnwattana, Patiwet
AU  - Wuttisarnwattana P
AUID- ORCID: 0000-0002-2687-3606
AD  - Department of Computer Engineering, Faculty of Engineering, Chiang Mai 
      University, Chiang Mai, 50200, Thailand. patiwet@eng.cmu.ac.th.
AD  - Biomedical Engineering Institute, Chiang Mai University, Chiang Mai, Thailand. 
      patiwet@eng.cmu.ac.th.
FAU - Eid, Saada
AU  - Eid S
AD  - Department of Pediatric Hematology and Oncology, Case Western Reserve University, 
      Cleveland, OH, USA.
FAU - Gargesha, Madhusudhana
AU  - Gargesha M
AD  - BioInVision, Inc., Mayfield Village, OH, USA.
FAU - Cooke, Kenneth R
AU  - Cooke KR
AD  - Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, School of 
      Medicine, Johns Hopkins University, Baltimore, MD, USA.
FAU - Wilson, David L
AU  - Wilson DL
AD  - Department of Biomedical Engineering, Case Western Reserve University, Cleveland, 
      OH, USA.
LA  - eng
GR  - MRG6080218/Thailand Research Fund/
GR  - R42-CA124270/NH/NIH HHS/United States
GR  - R41HD063241-01/NH/NIH HHS/United States
GR  - R42-CA124270/NH/NIH HHS/United States
GR  - R41HD063241-01/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20200226
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
SB  - IM
MH  - Animals
MH  - Bone Marrow/diagnostic imaging
MH  - Diagnostic Imaging/*methods
MH  - Disease Models, Animal
MH  - Female
MH  - Graft vs Host Disease/diagnostic imaging/therapy
MH  - Humans
MH  - Kidney/diagnostic imaging
MH  - Liver/diagnostic imaging
MH  - Lung/diagnostic imaging
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells
MH  - Mice, Inbred C57BL
MH  - Quantum Dots/administration & dosage
MH  - Spleen/diagnostic imaging
OTO - NOTNLM
OT  - Biodistribution
OT  - Cell detection
OT  - Cryo-imaging
OT  - Fluorescent imaging
OT  - Image processing
OT  - Stem cell
OT  - Stem cell homing
OT  - Stromal cell
OT  - Visualization
EDAT- 2020/02/28 06:00
MHDA- 2021/01/15 06:00
CRDT- 2020/02/28 06:00
PHST- 2019/08/20 00:00 [received]
PHST- 2020/02/21 00:00 [accepted]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
PHST- 2020/02/28 06:00 [entrez]
AID - 10.1007/s10439-020-02487-z [pii]
AID - 10.1007/s10439-020-02487-z [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2020 Jun;48(6):1702-1711. doi: 10.1007/s10439-020-02487-z. Epub 
      2020 Feb 26.