PMID- 32104226
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 3
DP  - 2020 Mar
TI  - Adipose tissue-derived stem cells modulate immune function in vivo and promote 
      long-term hematopoiesis in vitro using the aGVHD model.
PG  - 1725-1732
LID - 10.3892/etm.2020.8430 [doi]
AB  - The present study was designed to investigate the effect of adipose-derived stem 
      cells (ADSCs) on acute graft vs. host disease (aGVHD) and hematopoietic recovery 
      after allogeneic hematopoietic stem cell transplantation. ADSCs, bone 
      marrow-derived stem cells (BMSCs) and fibroblasts were cultured. ADSCs were 
      cocultured with hematopoietic stem/progenitor cells. Then, ADSCs were infused 
      into the aGVHD rat model. The survival of the rats was recorded. Livers and small 
      intestines were obtained from sacrificed rats for pathological examinations. 
      Expression of the Sry gene in recipient rats that survived longer than 21 days 
      was examined by real-time PCR to detect the presence of donor Y chromosome. 
      Expression of serum interferon (INF)-gamma and interleukin (IL)-4 was detected by 
      ELISA at 0, 7, 14, 21 and 50 days after transplantation. Transplantation of ADSCs 
      improved the survival of aGVHD rats. Survived ADSCs participated in hematopoietic 
      reconstitution in aGVHD rats. ADSCs decreased aGVHD severity by immunomodulation. 
      ADSCs support the proliferation of hematopoietic stem/progenitor cells in vitro. 
      The present study demonstrated that ADSCs may reduce aGVHD by influencing the 
      balance of IL-4 and INF-gamma and can promote long-term hematopoiesis.
CI  - Copyright: (c) Jiang et al.
FAU - Jiang, Ming
AU  - Jiang M
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
AD  - State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence 
      Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated 
      Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 
      830054, P.R. China.
FAU - Bi, Xiaojuan
AU  - Bi X
AD  - State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence 
      Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated 
      Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 
      830054, P.R. China.
FAU - Duan, Xianlin
AU  - Duan X
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
FAU - Pang, Nannan
AU  - Pang N
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
FAU - Wang, Hongbo
AU  - Wang H
AD  - State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence 
      Diseases in Central Asia, Clinical Medicine Institute, The First Affiliated 
      Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 
      830054, P.R. China.
FAU - Yuan, Hailong
AU  - Yuan H
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
FAU - Zhang, Rongyao
AU  - Zhang R
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
FAU - Cui, Lei
AU  - Cui L
AD  - Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
AD  - School of Medicine, Tongji University, Shanghai 200092, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200107
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7027076
OTO - NOTNLM
OT  - acute graft vs. host disease
OT  - adipose-derived stem cells
OT  - bone marrow stem cells
OT  - hematopoiesis support
OT  - immunomodulation
EDAT- 2020/02/28 06:00
MHDA- 2020/02/28 06:01
PMCR- 2020/01/07
CRDT- 2020/02/28 06:00
PHST- 2018/02/09 00:00 [received]
PHST- 2019/11/06 00:00 [accepted]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2020/02/28 06:01 [medline]
PHST- 2020/01/07 00:00 [pmc-release]
AID - ETM-0-0-8430 [pii]
AID - 10.3892/etm.2020.8430 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Mar;19(3):1725-1732. doi: 10.3892/etm.2020.8430. Epub 2020 Jan 
      7.