PMID- 32104269
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 3
DP  - 2020 Mar
TI  - Hyperhomocysteinemia inhibits tibial fracture healing in rats through PI3K/AKT 
      signaling pathway.
PG  - 2083-2088
LID - 10.3892/etm.2020.8412 [doi]
AB  - To explore the influence of hyperhomocysteinemia (hHcys) on the tibial fracture 
      healing in rats and its effect on the phosphatidylinositol 3-hydroxy kinase 
      (PI3K)/protein kinase B (AKT) signaling pathway. A total of 36 Sprague-Dawley 
      rats were randomly divided into sham group (n=12), tibial fracture group (n=12) 
      and hHcys + fracture group (n=12). The rats in tibial fracture group underwent 
      the tibial fracture surgery, while the model of tibial fracture and hHcys was 
      established in hHcys + fracture group. The level of plasma homocysteine (Hcy) in 
      each group was analyzed using the full-automatic biochemical analyzer, the 
      fracture stress biomechanical measurement was performed, and the ultimate bending 
      strength and torque were calculated. Moreover, the protein expressions of PI3K 
      and phosphorylated (p)-AKT in tibial tissues were detected using western 
      blotting, the messenger ribonucleic acid (mRNA) levels of Bcl-2 associated X 
      protein (Bax) and caspase-3 were detected using quantitative polymerase chain 
      reaction (qPCR), the apoptosis was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining, and the expressions 
      of inflammatory factors were detected via immunohistochemistry. Compared with 
      sham group, tibial fracture group and hHcys + fracture group had a significantly 
      increased level of plasma Hcy, significantly decreased ultimate bending strength 
      and torque, obviously decreased relative protein expressions of PI3K and p-AKT, 
      increased mRNA levels of Bax and caspase-3 and an increased expression of 
      pro-inflammatory factor tumor necrosis factor-alpha (TNF-alpha). Compared with tibial 
      fracture group, hHcys + fracture group had a higher level of plasma Hcy, lower 
      ultimate bending strength and torque, lower relative protein expressions of PI3K 
      and p-AKT, higher mRNA levels of Bax and caspase-3, a higher apoptosis rate and a 
      higher expression of TNF-alpha. hHcys blocks the downstream apoptotic signal 
      transduction, promotes apoptosis and inflammatory response, and affects fracture 
      healing through affecting the PI3K/AKT signaling pathway.
CI  - Copyright: (c) Liu et al.
FAU - Liu, Su
AU  - Liu S
AD  - Department of Orthopedics, The Affiliated Zhangjiagang Hospital of Soochow 
      University, Zhangjiagang, Jiangsu 215600, P.R. China.
FAU - Huang, Yunzong
AU  - Huang Y
AD  - Department of Orthopedics, The Affiliated Zhangjiagang Hospital of Soochow 
      University, Zhangjiagang, Jiangsu 215600, P.R. China.
FAU - Tian, Shoujing
AU  - Tian S
AD  - Department of Orthopedics, The Affiliated Zhangjiagang Hospital of Soochow 
      University, Zhangjiagang, Jiangsu 215600, P.R. China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Orthopedics, The Affiliated Zhangjiagang Hospital of Soochow 
      University, Zhangjiagang, Jiangsu 215600, P.R. China.
FAU - Xu, Youjia
AU  - Xu Y
AD  - Department of Orthopedics, The Second Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu 215004, P.R. China.
FAU - Ge, Jianfei
AU  - Ge J
AD  - Department of Orthopedics, The Affiliated Zhangjiagang Hospital of Soochow 
      University, Zhangjiagang, Jiangsu 215600, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200103
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7027308
OTO - NOTNLM
OT  - PI3K/AKT signaling pathway
OT  - hyperhomocysteinemia
OT  - inflammatory response
OT  - tibial fracture
EDAT- 2020/02/28 06:00
MHDA- 2020/02/28 06:01
PMCR- 2020/01/03
CRDT- 2020/02/28 06:00
PHST- 2019/09/16 00:00 [received]
PHST- 2019/11/11 00:00 [accepted]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2020/02/28 06:01 [medline]
PHST- 2020/01/03 00:00 [pmc-release]
AID - ETM-0-0-8412 [pii]
AID - 10.3892/etm.2020.8412 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Mar;19(3):2083-2088. doi: 10.3892/etm.2020.8412. Epub 2020 Jan 
      3.