PMID- 32104271
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 3
DP  - 2020 Mar
TI  - Fluvastatin protects myocardial cells in mice with acute myocardial infarction 
      through inhibiting RhoA/ROCK pathway.
PG  - 2095-2102
LID - 10.3892/etm.2020.8413 [doi]
AB  - Protective effect of fluvastatin (Flu) on myocardial cells in mice with acute 
      myocardial infarction (AMI) and the mechanism were explored. Forty C57B/L6 mice 
      in similar physiological status were selected and randomly divided into sham 
      operation (Sham) group (n=10), AMI group (n=10), Flu group (n=10) and Flu + 
      Angiotensin II (Ang II) (Ang II) group (n=10). The pathological changes in heart 
      tissues were detected via hematoxylin and eosin (H&E) staining, and apoptosis of 
      myocardial cells was detected via terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) assay. Moreover, the expression levels of 
      malondialdehyde (MDA) and superoxide dismutase (SOD) were determined using 
      relevant kits, and the expression levels of Ras homolog gene family 
      (Rho)-associated coiled-coil protein kinase 1 (ROCK1), ROCK2, B-cell lymphoma-2 
      (Bcl-2), Bcl-2 associated X protein (Bax) and nuclear factor-kappaB (NF-kappaB) in the 
      infarction region were determined using Western blotting. The infarction area in 
      mice in Flu group was significantly smaller than that in AMI group. In AMI group, 
      the level of MDA in the serum and infarction tissues was remarkably higher than 
      that in Sham group (P<0.05), while that of SOD significantly declined (P<0.05). 
      The level of MDA in Flu group was obviously lower than that in AMI group 
      (P<0.05). The expression levels of Bax, NF-kappaB, ROCK1 and ROCK2 were obviously 
      higher in AMI group than those in Sham group, while they were obviously lower in 
      Flu group than those in AMI group (P<0.05). After the Rho member A (RhoA)/ROCK 
      pathway agonist Ang II was added, the mitigation effect of Flu on myocardial 
      apoptosis in the infarction region in AMI mice was evidently weakened. Flu 
      mitigates AMI-induced myocardial apoptosis in mice, and the possible mechanism is 
      that the inflammatory and oxidative stress responses activated and mediated by 
      RhoA/ROCK are effectively inhibited.
CI  - Copyright: (c) Yi et al.
FAU - Yi, Zhenci
AU  - Yi Z
AD  - Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Ke, Jiaying
AU  - Ke J
AD  - Department of Marine Biology, Quanzhou Normal University, Quanzhou, Fujian 
      362000, P.R. China.
FAU - Wang, Yaoguo
AU  - Wang Y
AD  - Department of Emergency, The Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Cai, Kaijin
AU  - Cai K
AD  - Department of Emergency, The Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200103
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7027326
OTO - NOTNLM
OT  - RhoA/ROCK
OT  - aute myocardial infarction
OT  - fluvastatin
OT  - mouse
OT  - myocardial cell
EDAT- 2020/02/28 06:00
MHDA- 2020/02/28 06:01
PMCR- 2020/01/03
CRDT- 2020/02/28 06:00
PHST- 2019/09/27 00:00 [received]
PHST- 2019/11/25 00:00 [accepted]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2020/02/28 06:01 [medline]
PHST- 2020/01/03 00:00 [pmc-release]
AID - ETM-0-0-8413 [pii]
AID - 10.3892/etm.2020.8413 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Mar;19(3):2095-2102. doi: 10.3892/etm.2020.8413. Epub 2020 Jan 
      3.