PMID- 32104274
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 3
DP  - 2020 Mar
TI  - miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and 
      caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway.
PG  - 2119-2126
LID - 10.3892/etm.2020.8415 [doi]
AB  - Effect of micro ribonucleic acid (miR)-130a on neuronal apoptosis in rats with 
      cerebral infarction (CI) was studied to explore whether phosphatase and tensin 
      homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3-hydroxy kinase 
      (PI3K)/protein kinase B (Akt) is involved in the regulation of neuronal 
      apoptosis. Thirty-six Sprague-Dawley (SD) rats were randomly divided into blank 
      control group, model group and miR-130a low-expression group. miR-130a was 
      determined by quantitative polymerase chain reaction (qPCR), the content of tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and IL-10 was detected using the 
      enzyme-linked immunosorbent assay (ELISA) kits, and the neuronal apoptosis level 
      in each group was determined through terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. The neurobehavioral 
      score was significantly lower in model group than that in blank control group 
      (P<0.01), while it was significantly higher in miR-130a low-expression group than 
      that in model group (P<0.01). Compared with blank control group, the model group 
      had obviously increased content of TNF-alpha and IL-6 (P<0.01), decreased content of 
      IL-10 (P<0.01), more apoptotic neurons (P<0.01), higher expression of caspase-3 
      (P<0.01), and obviously lower Bcl-2/Bax (P<0.01). Moreover, expression of 
      phosphorylated (p)-PTEN, PI3K and p-Akt in brain tissues was remarkably lower in 
      the model group than those in the blank control group (P<0.01). The expression 
      level of miR-130a in brain tissues of CI rats is significantly increased. 
      miR-130a promotes the release of inflammatory factors and facilitates neuronal 
      apoptosis through suppressing the PTEN/PI3K/Akt signaling pathway.
CI  - Copyright: (c) Wang et al.
FAU - Wang, Yinming
AU  - Wang Y
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Gu, Junquan
AU  - Gu J
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Hu, Linlin
AU  - Hu L
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Kong, Liang
AU  - Kong L
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Wang, Tinggang
AU  - Wang T
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Di, Meiqi
AU  - Di M
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Li, Chaosheng
AU  - Li C
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
FAU - Gui, Shuhua
AU  - Gui S
AD  - Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 
      214041, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200103
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7027342
OTO - NOTNLM
OT  - PTEN/PI3K/Akt signaling pathway
OT  - apoptosis
OT  - cerebral infarction
OT  - miR-130a
EDAT- 2020/02/28 06:00
MHDA- 2020/02/28 06:01
PMCR- 2020/01/03
CRDT- 2020/02/28 06:00
PHST- 2019/10/08 00:00 [received]
PHST- 2019/12/04 00:00 [accepted]
PHST- 2020/02/28 06:00 [entrez]
PHST- 2020/02/28 06:00 [pubmed]
PHST- 2020/02/28 06:01 [medline]
PHST- 2020/01/03 00:00 [pmc-release]
AID - ETM-0-0-8415 [pii]
AID - 10.3892/etm.2020.8415 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Mar;19(3):2119-2126. doi: 10.3892/etm.2020.8415. Epub 2020 Jan 
      3.