PMID- 32108377 OWN - NLM STAT- MEDLINE DCOM- 20210303 LR - 20220414 IS - 1873-474X (Electronic) IS - 0736-5748 (Linking) VI - 80 IP - 3 DP - 2020 May TI - Elevation of stromal cell-derived factor 1 and C-X-C chemokine receptor type 4 in white matter damage treatment with recombinant human erythropoietin and human umbilical cord mesenchymal stem cells in a rat model of preterm birth. PG - 247-256 LID - 10.1002/jdn.10021 [doi] AB - OBJECTIVES: To investigate the role of stromal cell-derived factor 1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) in the premature brain with white matter damage (WMD) undergoing treatment with human umbilical cord mesenchymal stem cells (hUC-MSCs) and recombinant human erythropoietin (rhEPO). EXPERIMENTAL DESIGN: Three-day-old Sprague-Dawley (SD) rats were randomly divided into sham operation group, hypoxia-ischemia (HI) group, rhEPO treated HI group, hUC-MSCs treated HI group, and rhEPO + hUC-MSCs treated HI group. WMD was established in all groups except the Sham group. SDF-1 and CXCR-4 levels in each group were detected at postnatal day (P) 5, P7, and P14. Pathological changes were assessed via HE staining at P14 and neuroethological tests were performed at P28. OBSERVATIONS AND CONCLUSIONS: The rhEPO and hUC-MSCs intervention reduced injury area, increased body weight at P7, and improved neurobehavioral scores at P28. Furthermore, their combined use proved even more beneficial. SDF-1 levels in the rhEPO group were higher than those in the other groups and highest in the hUC-MSCs + rhEPO group (all p < .01). SDF-1 levels in the hUC-MSCs + rhEPO and rhEPO groups were increased at P5 and reached a peak at P7. CXCR-4 levels in the hUC-MSCs group were higher than those in the other groups and highest in the hUC-MSCs + rhEPO group (all p < .01). CXCR-4 levels were also increased at P5 and highest at P14. SIGNIFICANCE: hUC-MSCs + rhEPO might reduce nerve cell damage and improve neurobehavioral development, in connection with increased SDF-1 and CXCR-4 expression, in premature rats with WMD due to hypoxic-ischemic injury. CI - (c) 2020 International Society for Developmental Neuroscience. FAU - Yin, Liping AU - Yin L AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Wang, Shiyu AU - Wang S AD - Department of Pediatrics, Xuanwu Hospital Medical University, Beijing, China. FAU - Zhang, Ning AU - Zhang N AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Bai, Xiang AU - Bai X AD - Neonatal Intensive Care Unit, Gansu Provincial Maternity and Child-care Hospital, Lanzhou, China. FAU - Xie, Jiali AU - Xie J AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Wen, Quan AU - Wen Q AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Huang, Li AU - Huang L AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Qian, Lijuan AU - Qian L AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. FAU - Jiang, Li AU - Jiang L AUID- ORCID: 0000-0003-0377-2471 AD - Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing, China. AD - Teaching and Research Section of Pediatrics, Medical College, Southeast University, Nanjing, China. LA - eng GR - BK20151420/Natural Science Foundation of Jiangsu Province/ GR - 81771628/National Natural Science Foundation of China/ PT - Journal Article DEP - 20200325 PL - United States TA - Int J Dev Neurosci JT - International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience JID - 8401784 RN - 0 (Chemokine CXCL12) RN - 0 (Cxcr4 protein, rat) RN - 0 (Receptors, CXCR4) RN - 0 (Recombinant Proteins) RN - 11096-26-7 (Erythropoietin) SB - IM MH - Animals MH - Behavior, Animal/physiology MH - Body Weight/physiology MH - Chemokine CXCL12/*metabolism MH - Disease Models, Animal MH - Erythropoietin/administration & dosage/*therapeutic use MH - Female MH - *Mesenchymal Stem Cell Transplantation MH - Mesenchymal Stem Cells/physiology MH - Pregnancy MH - Premature Birth/drug therapy/*metabolism/pathology MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, CXCR4/*metabolism MH - Recombinant Proteins/*therapeutic use MH - White Matter/*metabolism/pathology OTO - NOTNLM OT - CXC family chemokine 4 OT - human umbilical cord mesenchymal stem cells OT - hypoxia-ischemia OT - premature rats OT - recombinant human erythropoietin OT - stromal cell-derived factor 1 OT - white matter damage EDAT- 2020/02/29 06:00 MHDA- 2021/03/04 06:00 CRDT- 2020/02/29 06:00 PHST- 2020/01/14 00:00 [received] PHST- 2020/02/19 00:00 [revised] PHST- 2020/02/24 00:00 [accepted] PHST- 2020/02/29 06:00 [pubmed] PHST- 2021/03/04 06:00 [medline] PHST- 2020/02/29 06:00 [entrez] AID - 10.1002/jdn.10021 [doi] PST - ppublish SO - Int J Dev Neurosci. 2020 May;80(3):247-256. doi: 10.1002/jdn.10021. Epub 2020 Mar 25.