PMID- 32110275
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 12
IP  - 1
DP  - 2020 Jan 26
TI  - Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone 
      marrow-derived stem cells on rats' critical-sized mandibular defects.
PG  - 55-69
LID - 10.4252/wjsc.v12.i1.55 [doi]
AB  - BACKGROUND: Critically sized bone defects represent a significant challenge to 
      orthopaedic surgeons worldwide. These defects generally result from severe trauma 
      or resection of a whole large tumour. Autologous bone grafts are the current gold 
      standard for the reconstruction of such defects. However, due to increased 
      patient morbidity and the need for a second operative site, other lines of 
      treatment should be introduced. To find alternative unconventional therapies to 
      manage such defects, bone tissue engineering using a combination of suitable 
      bioactive factors, cells, and biocompatible scaffolds offers a promising new 
      approach for bone regeneration. AIM: To evaluate the healing capacity of 
      platelet-rich fibrin (PRF) membranes seeded with allogeneic mesenchymal bone 
      marrow-derived stem cells (BMSCs) on critically sized mandibular defects in a rat 
      model. METHODS: Sixty-three Sprague Dawley rats were subjected to bilateral bone 
      defects of critical size in the mandibles created by a 5-mm diameter trephine 
      bur. Rats were allocated to three equal groups of 21 rats each. Group I bone 
      defects were irrigated with normal saline and designed as negative controls. 
      Defects of group II were grafted with PRF membranes and served as positive 
      controls, while defects of group III were grafted with PRF membranes seeded with 
      allogeneic BMSCs. Seven rats from each group were killed at 1, 2 and 4 wk. The 
      mandibles were dissected and prepared for routine haematoxylin and eosin (HE) 
      staining, Masson's trichrome staining and CD68 immunohistochemical staining. 
      RESULTS: Four weeks postoperatively, the percentage area of newly formed bone was 
      significantly higher in group III (0.88 +/- 0.02) than in groups I (0.02 +/- 0.00) 
      and II (0.60 +/- 0.02). The amount of granulation tissue formation was lower in 
      group III (0.12 +/- 0.02) than in groups I (0.20 +/- 0.02) and II (0.40 +/- 0.02). The 
      number of inflammatory cells was lower in group III (0.29 +/- 0.03) than in groups 
      I (4.82 +/- 0.08) and II (3.09 +/- 0.07). CONCLUSION: Bone regenerative quality of 
      critically sized mandibular bone defects in rats was better promoted by PRF 
      membranes seeded with BMSCs than with PRF membranes alone.
CI  - (c)The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights 
      reserved.
FAU - Awadeen, Muhammad A
AU  - Awadeen MA
AD  - Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University 
      for Science and Technology, Mansoura 11152, Egypt.
FAU - Al-Belasy, Fouad A
AU  - Al-Belasy FA
AD  - Department of Oral Surgery and Anesthesia, Faculty of Oral and Dental Medicine, 
      Delta University for Science and Technology, Mansoura 11152, Egypt.
FAU - Ameen, Laila E
AU  - Ameen LE
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura 
      35516, Egypt.
FAU - Helal, Mohamad E
AU  - Helal ME
AD  - Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura 
      35516, Egypt.
FAU - Grawish, Mohammed E
AU  - Grawish ME
AD  - Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University 
      for Science and Technology, Mansoura 11152, Egypt.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC7031757
OTO - NOTNLM
OT  - Bone marrow-derived stem cells
OT  - Critical-sized mandibular defects
OT  - Histological and immunohistochemical staining
OT  - Platelet-rich fibrin membrane
OT  - Rats
COIS- Conflict-of-interest statement: Authors declare no conflict of interests for this 
      article.
EDAT- 2020/02/29 06:00
MHDA- 2020/02/29 06:01
PMCR- 2020/01/26
CRDT- 2020/02/29 06:00
PHST- 2019/03/07 00:00 [received]
PHST- 2019/05/13 00:00 [revised]
PHST- 2019/11/29 00:00 [accepted]
PHST- 2020/02/29 06:00 [entrez]
PHST- 2020/02/29 06:00 [pubmed]
PHST- 2020/02/29 06:01 [medline]
PHST- 2020/01/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v12.i1.55 [doi]
PST - ppublish
SO  - World J Stem Cells. 2020 Jan 26;12(1):55-69. doi: 10.4252/wjsc.v12.i1.55.