PMID- 32111303
OWN - NLM
STAT- MEDLINE
DCOM- 20200727
LR  - 20201028
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 99
IP  - 3
DP  - 2020 Mar
TI  - Impact of in ovo administered pioneer colonizers on intestinal proteome on day of 
      hatch.
PG  - 1254-1266
LID - S0032-5791(19)44174-6 [pii]
LID - 10.1016/j.psj.2019.10.017 [doi]
AB  - Pioneer colonization of the gastrointestinal tract (GIT) by bacteria is thought 
      to have major influence on neonatal tissue development. Previous studies have 
      shown in ovo inoculation of embryos with saline (S), species of Citrobacter (C, 
      C2), or lactic acid bacteria (L) resulted in an altered microbiome on day of the 
      hatch (DOH). The present study investigated GIT proteomic changes at DOH in 
      relation to different inoculations. Embryos were inoculated in ovo with S or 
       approximately 10(2) cfu of C, C2, or L at 18 embryonic days. On DOH, the GIT was collected, 
      and tissue proteins were extracted for analysis via tandem mass spectrometry. A 
      total of 493 proteins were identified for differential comparison with S at 
      P </= 0.10. Different levels were noted in 107, 39, and 78 proteins in C, C2, and L 
      groups, respectively, which were uploaded to Ingenuity Pathway Analysis to 
      determine canonical pathways and biological functions related to these changes. 
      Three members of the cytokine family (interleukin [IL]-1beta, IL6, and Oncostatin M) 
      were predicted to be activated in C2, indicated with Z-score >/= 1.50, which 
      suggested an overall proinflammatory GIT condition. This was consistent with the 
      activation of the acute-phase response signaling pathway seen exclusively in C2 
      (Z-score = 2.00, P < 0.01). However, activation (Z-score = 2.00) of IL-13, 
      upregulation of peroxiredoxin-1 and superoxide dismutase 1, in addition to 
      activation of nitric oxide signaling in the cardiovascular system of the L 
      treatment may predict a state of increased antioxidant capacity and decreased 
      inflammatory status. The nuclear factor erythroid 2-related factor 2 
      (NRF2)-mediated oxidative stress response (Z-score = 2.00, P < 0.01) was 
      predicted to be upregulated in C which suggested that chicks were in an 
      inflammatory state and associated oxidative stress, but the impact of these 
      pathways differed from that of C2. These changes in the proteome suggest that 
      pioneer colonizing microbiota may have a strong impact on pathways associated 
      with GIT immune and cellular development.
CI  - Copyright (c) 2019. Published by Elsevier Inc.
FAU - Wilson, K M
AU  - Wilson KM
AD  - Department of Animal Science, The Ohio State University, Columbus, OH.
FAU - Rodrigues, D R
AU  - Rodrigues DR
AD  - Department of Animal Science, The Ohio State University, Columbus, OH.
FAU - Briggs, W N
AU  - Briggs WN
AD  - Department of Animal Science, The Ohio State University, Columbus, OH.
FAU - Duff, A F
AU  - Duff AF
AD  - Department of Animal Science, The Ohio State University, Columbus, OH.
FAU - Chasser, K M
AU  - Chasser KM
AD  - Department of Animal Science, The Ohio State University, Columbus, OH.
FAU - Bottje, W G
AU  - Bottje WG
AD  - Department of Poultry Science, University of Arkansas, Fayetteville, AR.
FAU - Bielke, L R
AU  - Bielke LR
AD  - Department of Animal Science, The Ohio State University, Columbus, OH. Electronic 
      address: bielke.1@osu.edu.
LA  - eng
PT  - Journal Article
DEP - 20200130
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - *Bacterial Load
MH  - Chickens/metabolism/*microbiology
MH  - Gastrointestinal Microbiome/*physiology
MH  - Gastrointestinal Tract/microbiology/*physiology
MH  - Proteome/*metabolism
PMC - PMC7587751
OTO - NOTNLM
OT  - gastrointestinal tract
OT  - inflammation
OT  - pioneer colonizers
OT  - proteome
EDAT- 2020/03/01 06:00
MHDA- 2020/07/28 06:00
PMCR- 2020/01/30
CRDT- 2020/03/01 06:00
PHST- 2019/08/05 00:00 [received]
PHST- 2019/10/02 00:00 [revised]
PHST- 2019/10/02 00:00 [accepted]
PHST- 2020/03/01 06:00 [entrez]
PHST- 2020/03/01 06:00 [pubmed]
PHST- 2020/07/28 06:00 [medline]
PHST- 2020/01/30 00:00 [pmc-release]
AID - S0032-5791(19)44174-6 [pii]
AID - 10.1016/j.psj.2019.10.017 [doi]
PST - ppublish
SO  - Poult Sci. 2020 Mar;99(3):1254-1266. doi: 10.1016/j.psj.2019.10.017. Epub 2020 
      Jan 30.